A5014727961- RNA Interference and Gene Delivery
- interferon and immune responses
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Viral Infections and Immunology Research
- HIV/AIDS drug development and treatment
Sechenov University
2022-2023
Objectives. Evaluation of changes in the viral activity influenza A/WSN/33 after complex knockdown combinations cellular genes FLT4, Nup98 and Nup205 human lung cell culture A549. Methods. The work was carried out using equipment Center for Collective Use I. Mechnikov Research Institute Vaccines Sera, Russia. authors performed transfection small interfering ribonucleic acid (siRNA) complexes that cause simultaneous disruption expression Nup98, Nup205. Within three days from moment infection,...
Introduction. Influenza is one of the most pressing global health problems. Despite wide range available anti-influenza drugs, viral drug resistance an increasing concern and requires search for new approaches to overcome it. A promising solution development drugs with action that based on inhibition activity cellular genes through RNA interference.
 Aim. Evaluation in vivo preventive potential miRNAs directed FLT4, Nup98 Nup205 against influenza infection.
 Materials methods. The...
Influenza is one of the most urgent global health problems today. The influenza virus has immunosuppressive properties, which can lead to development secondary immunodeficiencies, interfering with functioning interferon system activation, thus leading impaired production pro-inflammatory cytokines. IL-1 important player in antiviral immunity. This cytokine plays an role boosting expression MCP-1 and MCP-3 genes maturation macrophages dendritic cells. Induction occurs due interaction ligand...
Objectives . To evaluate the dynamics of expression level IL-1β and IL-28β (IFN-λ3) genes as a result complex knockdown some cellular genes, whose products play an important role in reproduction influenza virus. Methods Following collection virus-containing liquid cell lysate within three days from moment transfection infection, intensity viral was assessed using cytopathic effect titration method. The concentration ribonucleic acid (vRNA) change were determined by real-time reverse...