A5003201899- Hepatitis Viruses Studies and Epidemiology
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Liver Diseases and Immunity
- Veterinary medicine and infectious diseases
Ministry of Health
2019-2020
Chinese Preventive Medicine Association
2017
Despite the fact that Kyrgyz Republic (KR) belongs to highly endemic regions of world for hepatitis E, true extent spread this infection in country remains poorly understood. It was estimated prevalence serological markers E virus (HEV) among patients with acute viral (AVH) from a high level seroprevalence previously established by us. Blood sera samples who were admitted hospitals Kyrgyzstan period 2018-2019 examined enzyme immunoassay method using kits «DS-ELISA-Anti-HEV-IgG» and...
The diagnostic efficacy of methods for hepatitis E serodiagnostic varies over a wide range; therefore, the combined use tests various formats is recommended. aim research was to develop test system detection IgG antibodies virus (HEV) in human serum by linear immunoassay (LIA).Serum samples from patients with and healthy individuals were tested using commercial enzyme-linked immunosorbent assay systems presence viral agents causing other infections associated liver pathology. Recombinant...
Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical Results. Using HEV DNA copy subgenomic RNA we made E.coli strains producing recombinabt protein, containing C-terminal fragment ORF2 protein fused to beta-galactosidase. Recombinant had been isolated from inclusion bodies...
Purpose – To study the prevalence of antibodies to hepatitis E virus (AB-HEV) among population Kyrgyzstan. Materials and methods: Blood sera samples healthy people patients from hospitals Kyrgyzstan were examined by enzyme immunoassay method using tests «DS-ELISA-Anti-HEV-IgG» «DS-ELISA-ANTI-HEVIgM» (RPC Diagnostic Systems, Russia). Results: AB-HEV detected in 482 2279 studied (21.1%). The greatest number seropositive specimens found Osh Batken regions Bishkek city. Most often, persons age 1...
Aim. The development of the hepatitis E virus (HEV) genotype 1 full-size ORF3 recombinant polypeptide. Materials and methods. Escherichia coli strains, plasmid vectors, serological clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical Results. HEV RNA had been isolated from samples collected in Kyrgyzstan. DNA copy subgenomic cloned used for further E.coli strains producing protein fused to beta-galactosidase. Codons optimization method was...
Цель. Выявление особенностей течения COVID-19 у детей с коморбидной патологией на основе анализа клинико-эпидемиологических, лабораторных и инструментальных болезни. Материалы методы. В исследование вошли 249 лабораторно подтвержденным методом ПЦР COVID-19. Пациенты были госпитализированы в Республиканскую специализированную больницу «Зангиата-1». Результаты. Чаще встречались пациенты заболеваниями сердечно-сосудистой системы – 49,7%, органов дыхания 1,7%, эндокринными 7,6%, другими 26,5%....