A5003371095- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- SARS-CoV-2 detection and testing
- RNA regulation and disease
- Viral Infections and Outbreaks Research
- Vaccine Coverage and Hesitancy
- Endoplasmic Reticulum Stress and Disease
- Animal Virus Infections Studies
- COVID-19 diagnosis using AI
- CRISPR and Genetic Engineering
- Influenza Virus Research Studies
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- COVID-19 epidemiological studies
- Vector-Borne Animal Diseases
- interferon and immune responses
- Immune responses and vaccinations
- Respiratory viral infections research
- Mosquito-borne diseases and control
Chumakov Institute of Poliomyelitis and Viral Encephalitides
2020-2025
Russian Academy of Sciences
2012-2025
Centre for Research and Development
2022
Lomonosov Moscow State University
2012
The unprecedented in recent history global COVID-19 pandemic urged the implementation of all existing vaccine platforms to ensure availability vaccines against every country world. Despite multitude high-quality papers describing clinical trials different products, basic detailed data on general toxicity, reproductive immunogenicity, protective efficacy and durability immune response animal models are scarce. Here, we developed a β-propiolactone-inactivated whole virion CoviVac assessed its...
ObjectivesThe outbreak of coronavirus disease 2019 (COVID-19) started in December China and then spread worldwide over the following months, involving 188 countries. The objective this study was to determine molecular epidemiology COVID-19 Russia.MethodsIn study, two severe acute respiratory syndrome 2 (SARS-CoV-2) strains were isolated genetically characterized. A phylogenetic analysis all available Russian sequences performed these compared epidemiological data on incidence evaluate...
Effective response to emerging pandemic threats is complicated by the need develop specific vaccines and other medical products. The availability of broadly countermeasures that could be deployed early in significantly alter its course save countless lives. Live attenuated (LAVs) were shown induce non-specific protection against a broad spectrum off-target pathogens stimulating innate immune responses. purpose this study was evaluate effect immunization with bivalent Oral Poliovirus Vaccine...
Vaccination is the most effective strategy for fighting viral diseases, with both live and inactivated vaccines remaining crucial despite advancements in subunit vaccine technologies. A key player production Vero cell line, derived from kidney cells of African green monkey, which essential manufacturing against diseases like polio, rabies, yellow fever, COVID-19. The efficiency cultivation directly impacts production, often utilizing bioreactors ranging small (1–10 L) to large (up several...
A key contributor to the pathogenicity of viruses is their interaction with cellular defense mechanisms, including UPR (unfolded protein response) that counteracts accumulation misfolded proteins in endoplasmic reticulum (known as ER stress). One branches mediated by IRE1 (inositol-requiring enzyme 1) protein, which possesses kinase and RNase activities facilitate unconventional cytoplasmic splicing XBP1 mRNA, leading upregulation transcription factor. In this study, we demonstrate...
Relevance . During 2021 and early 2022, the prevalence of COVID-19 in child population was 9.5%, frequency hospitalizations children adolescents increased, fatal outcomes began to be recorded. And 2022–2023, proportion infected with increased 18%. At same time, vaccination remains most effective way protect against infection. Studying safety vaccines for prevention coronavirus infection is an urgent task. Objective study To a vaccine new immature animals. Materials methods The conducted on...
We present the results of a randomized, double-blind, placebo-controlled, multi-center clinical trial phase I/II tolerability, safety, and immunogenicity inactivated whole virion concentrated purified coronavirus vaccine CoviVac in volunteers aged 18-60 open comparative IIb 60 years older. The safety was assessed 400 age cohort who received two doses (n = 300) or placebo 100) 200 60+ all whom three vaccine. studied has shown good tolerability safety. No deaths, serious adverse events (AEs),...
Abstract The severe COVID‐19 pandemic drives the research toward SARS‐CoV‐2 virion structure and possible therapies against it. Here, we characterized β‐propiolactone inactivated virions using transmission electron microscopy (TEM) atomic force (AFM). We compared samples purified by two consecutive chromatographic procedures (size exclusion chromatography [SEC], followed ion‐exchange [IEC]) with ultracentrifugation. prepared SEC IEC retained more spikes on surface than ones...
Many viruses are known to trigger endoplasmic reticulum (ER) stress in host cells, which turn can develop a protective unfolded protein response (UPR). Depending on the conditions, UPR may lead either cell survival or programmed death. One of three branches involves upregulation Xbp1 transcription factor caused by unconventional cytoplasmic splicing its mRNA. This process is accomplished phosphorylated form endoribonuclease/protein kinase Ire1/ERN1. Here, we show that phosphorylation Ire1...
The EMCV L and 2A proteins are virulence factors that counteract host cell defense mechanisms. Both exhibit antiapoptotic properties, but the available data were obtained in different lines under incomparable conditions. This study is aimed at checking role of these choice death type three using mutants lacking functional L, 2A, both together. We have found non-essential for viral replication HeLa, BHK, RD lines, as evidenced by viability virus absence proteins. L-deficient infection led to...
Global polio eradication requires both safe and effective vaccines, production processes. Sabin oral poliomyelitis vaccine (OPV) strains can evolve to virulent viruses result in outbreaks, conventional inactivated (Salk-IPV) includes accumulation of large stocks neurovirulent wild polioviruses. Therefore, IPV based on attenuated OPV seems a viable option. To increase the global supply affordable still not-polio free world we developed an made from strains–PoliovacSin. Clinical trials...
ABSTRACT We present the results of a randomized, double-blind, placebo-controlled, multi-center clinical trial tolerability, safety, and immunogenicity inactivated whole virion concentrated purified coronavirus vaccine CoviVac in adult volunteers aged 18-60. Safety was assessed 398 who received two doses (n=298) or placebo (n=100). The studied has shown good tolerability safety. No deaths, serious adverse events (AE), other significant AE related to vaccination have been detected. most...
ABSTRACT Viruses often elicit cell injury (cytopathic effect [CPE]), a major cause of viral diseases. CPE is usually considered to be prerequisite for and/or consequence efficient growth. Recently, we proposed that may largely due host defensive and antidefensive activities. This study aimed check the validity this proposal by using as model HeLa cells infected with mengovirus (MV). As showed previously, infection these wild-type MV resulted in necrosis, whereas mutant incapacitated...
Abstract Vaccines are proven to be an effective tool in prophylaxis of severe COVID-19, but emerging mutated SARS-CoV-2 variants constantly challenge vaccines’ protectivity. We have evaluated the ability sera from individuals vaccinated with two inactivated vaccine CoviVac and COVID-19 convalescents (May-December 2020) neutralize Delta Omicron. Four groups serum samples (CoviVac vaccinees; convalescents; mice immunized preparations based on prototype B.1.1 strain variant) were virus...
Introduction. Infection of cells with encephalomyocarditis virus type 1 (EMCV-1, Cardiovirus A: Picornaviridae) is accompanied by suppression cellular protein synthesis. The main role in the inhibition translation assigned to L and 2A «security» proteins. mechanism possible influence on unknown. There are hypotheses about efficiency cap-dependent translation, which based interaction factors ribosome subunits. However, available experimental data contradictory, obtained using different...
Introduction. Since the development of inactivated polio vaccines, different stages production process have been changed and improved. Current vaccines based on both wild attenuated strains includes several technological stages, one which is concentration virus-containing liquid, ensures poliovirus concentration, purification liquid from a significant part ballast components.Research objective to compare characteristics ultrafiltration membranes select that provide optimal value type 1...
In the production of anti-virus vaccines, replacement wild-type with attenuated strains is becoming relevant. Sabin are most suitable candidates for this in inactivated poliovirus vaccines. The main problem development methods purification viral particles preservation their stability and antigenic properties. We have compared sorbents used gel-filtration concentrates selected a chromatographic resin, Sephacryl S-300HR, which provides high yield target product its purity both during...
Relevance . The production and quality control of any drugs are strictly regulated. In the case antiviral vaccines, requirements for their safety protective activity getting tougher every year. Vaccine manufacturers face three tasks: ensuring high immunogenicity, availability drug. During development immunobiological drugs, must demonstrate sufficient purification from technological impurities to ensure purity Technological directly depend on process expression systems used. Vero cell line...
Various types of COVID-19 vaccines, including adenovirus, mRNA, and inactivated ones, have been developed approved for clinical use worldwide. Inactivated vaccines are produced using a proven technology that is widely used the production prevention control infectious diseases, influenza poliomyelitis. The development whole-virion commonly includes several stages: cellular viral biomass in cell culture; inactivation virus; filtration ultrafiltration; chromatographic purification antigen;...
BACKGROUND: Despite the large-scale development of vaccines against coronavirus infection, there is still no complete information about antigenic structure SARS-CoV-2 virus particles. This article describes a method obtaining pure concentrated whole-virion sample that can be used in various studies. AIM: The goal to develop an optimal purification inactivated order obtain standard with parameters purity and antigen content sufficient for structural immunological MATERIALS AND METHODS: To we...