Cutaneous squamous cell carcinoma (CSCC) is a common type of skin malignancy. MicroRNA-221 (miRNA-221) critical non-coding RNA in tumor initiation and progression. However, the molecular mechanisms miRNA-221 development CSCC remain unknown. This study investigated expression its potential biological functions. MTT assay, colony PCR, Western blot were adopted. In this study, was significantly higher tissues lines than normal cells (P < 0.05). Further functional experiments indicated that...

Purpose Because of differences in therapy for first-time perianal abscess, a wide range recurrences and/or development fistula-in-ano (RF) rates have been reported. The indication determining when surgical intervention is needed remains obscure and controversial. This retrospective study sought to compare outcomes conservative treatment with those after incision drainage (ID) determine the optimal time intervention. Methods A total 697 patients abscess were included this study. median...

Background: Fibrinogen albumin ratio (FAR) is significantly correlated with the severity and prognosis of cardiovascular disease (CVD). Arterial stiffness an early lesion CVD, but no studies have examined correlation between arterial FAR. This study aimed to examine relationship FAR in patients type 2 diabetes (T2D), as measured by brachial-ankle pulse wave velocity (baPWV). Methods: In this cross-sectional investigation, T2D were enrolled January 2021 April 2022. each patient, levels...

Keloid disease (KD) is a benign fibroproliferative scarring condition of unknown etiopathogenesis. Plasminogen activator inhibitor-1 (PAI-1) and vitamin D receptor (VDR) have been shown to play important roles in the progression tissue fibrosis; therefore, both these genes are potential susceptibility for KD. We aimed determine whether gene expression levels PAI-1 VDR altered Chinese KD patients. measured PAI human peripheral blood lymphocytes 236 patients with keloid 219 age- sex-matched...

The incidence of cutaneous squamous cell carcinoma (CSCC), a malignant tumor that threatens human life, is increasing every year, and yet its pathogenesis still unclear. This study found long noncoding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) was abnormally expressed in CSCC. However, the biochemical mechanisms lncRNA NEAT1 carcinogenesis development cancer remain Fluorescence quantitative polymerase chain reaction (qPCR) conducted to determine expression CSCC...

Biliary atresia (BA) is a childhood liver disease characterized by fibrous obstruction and of the extrahepatic biliary system one most common serious disorders in infants. Significant inflammation fibrosis tract are prominent features, regardless initial damage to BA. Abnormalities innate or adaptive immunity have been found human patients mouse models We previously reported that children with BA had abnormal lipid metabolism, including free serum carnitine.To study gene protein expression...

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and disulfide (GSSG) using tandem spectrometry (MS/MS) detecting G6PD deficiency.The concentration of GSH the GSH/GSSG ratio in newborn dry-blood-spot (DBS) blood plus sodium citrate test confirmation were examined by MS/MS labeled glycine as an internal standard.G6PD-deficient newborns had lower content (242.9 ± 15.9...

To detect the composition of gut microbiota in biliary atresia after Kasai surgery.Infants within six months operation who were diagnosed by cholangiography at Shanghai Children's Hospital enrolled study. Fecal samples collected from diapers, placed into sterile tubes inpatient department or outpatient and frozen -80°C half an hour. The was detected 16S rRNA sequences. Then, patients that followed up to one year suffered cholangitis least time grouped BAcho group, others BAnoncho group.Nine...

Minichromosome maintenance (MCM) is known for participating in cell cycle progression, as well DNA replication. While the diverse expression patterns and prognostic values of MCMs melanoma still remained unclear.In present study, transcriptional clinical profiles were explored patients with from multiple databases, including GEO, TCGA, ONCOMINE, GEPIA, UALCAN, cBioPortal, TIMER databases.We found that elevated expressions MCM2-6 MCM10 significantly expressed compared to normal skin. High...

ZCVI4-2 is a newly developed furoxan-based nitric oxide-releasing derivative of oleanolic acid. It exhibited strong cytotoxicity against human hepatocellular carcinoma (HCC) in vitro and significantly inhibited the growth HCC tumors vivo. However, its low aqueous solubility toxicity due to fast release oxide (NO) blood challenged formulation. In present investigation, interaction characteristics with bovine serum albumin (BSA) were studied by fluorescence spectrometry, synchronous spectra...

<b>Purpose</b> The aim of this article is to differentiate neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) from biliary atresia (BA) total hexose. <b>Methods</b> A 11 patients with NICCD, 29 BA, and 4,898 children as controls were involved in study. blood concentration amino acids, carnitine, acylcarnitines, hexose measured dry spots (DBS) using tandem mass spectrometry (MS/MS). <b>Results</b> In the (15.3 ± 9.0 mmol/L vs. 7.3 2.7 mmol/L; <i>p</i> < 0.001), citrulline...

Sphingosine kinase 1 (SphK1) is overexpressed in skin squamous cell carcinoma (SCC). It has emerged as a novel therapeutic oncotarget. The current study identified SphK1-targeting microRNA, microRNA-6784 (miR-6784). Here, we show that miR-6784 located at the cytoplasm of A431 SCC cells. directly binds to SphK1 mRNA. Ectopic overexpression inhibited 3'-untranslated region (UTR) luciferase activity and downregulated its expression. Moreover, caused ceramide accumulation Functional studies...

Objective. This study investigated whether lncRNA NEAT1 could inhibit the proliferation of cutaneous squamous cell carcinoma (CSCC) cells by targeting miR-342-3p/CUL4B, thereby affecting occurrence and development CSCC. Methods. Fluorescence quantitative PCR was used to detect expression miR-42-3p in skin adjacent tissues. Bioinformatics software luciferase reporter gene assay were analyze association miR-342-3p. The effect overexpression or knockdown miR-342-3p on CSCC examined MTT colony...

<italic>Background</italic>: because hyperbilirubinemia is harmful and associated with many kinds of diseases, especially in neonates, it necessary to have methods available detect bilirubin blood as early possible.

To investigate the efficiency of free carnitine, unconjugated bilirubin (UBIL), monoglucuronide (BMG), and diglucuronide (BDG) in dry blood spots (DBSs) measured using tandem mass spectrometry (MS/MS) for screening biliary atresia (BA). All patients with BA, residing Shanghai, were collected from four different children's hospitals Shanghai January 1, 2015, to June 30, 2017. UBILMS, BMG, BDG, carnitine DBS samples 48 10,008 pediatric patients, 52,862 newborns MS/MS. Conjugated was by MS/MS...

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Objective To provide methodological references for laboratories to carry out newborn screening disorders of amino acid metabolism,we compared the difference and distribution ten acids including alanine (Ala),arginine (Arg),citrulline (Cit),glycine (Gly),leucine (Leu),methionine (Met),ornithine (Orn),phenylalanine (Phe),tyrosine (Tyr),and valine (Val) from dried blood spots specimen using derivatization or non-derivatization as sample preparation methods. Method It is a comparative...