A5019102142- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Drug Transport and Resistance Mechanisms
- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Antifungal resistance and susceptibility
- Cystic Fibrosis Research Advances
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
- Essential Oils and Antimicrobial Activity
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Nosocomial Infections in ICU
- Sphingolipid Metabolism and Signaling
- Bacterial Infections and Vaccines
- Drug-Induced Adverse Reactions
- Vibrio bacteria research studies
- Tuberculosis Research and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Cellular transport and secretion
UCLouvain
2016-2025
Elisabeth-TweeSteden Ziekenhuis
2022
Qatar University
2022
European Society of Clinical Microbiology and Infectious Diseases
2018
UCB Pharma (Belgium)
2014
University College Lahore
2014
University of Bari Aldo Moro
2010-2013
Université Libre de Bruxelles
2010
UCL Australia
2010
Weatherford College
2008
Abstract Bacterial persister cells are phenotypic variants that exhibit a transient non-growing state and antibiotic tolerance. Here, we provide in vitro evidence of Staphylococcus aureus persisters within infected host cells. We show the bacteria surviving treatment persisters, displaying biphasic killing reaching uniformly non-responsive, non-dividing when followed at single-cell level. This phenotype is stable but reversible upon removal. Intracellular S. remain metabolically active...
The pharmacodynamic properties governing the activities of antibiotics against intracellular Staphylococcus aureus are still largely undetermined. Sixteen seven different pharmacological classes (azithromycin and telithromycin [macrolides]; gentamicin [an aminoglycoside]; linezolid oxazolidinone]; penicillin V, nafcillin, ampicillin, oxacillin [beta-lactams]; teicoplanin, vancomycin, oritavancin [glycopeptides]; rifampin ansamycin]; ciprofloxacin, levofloxacin, garenoxacin, moxifloxacin...
In contrast to currently marketed fluoroquinolones, which are zwitterionic, delafloxacin is an investigational fluoroquinolone with anionic character that highly active against Gram-positive bacteria. We have examined the effect of acidic pH on its accumulation in Staphylococcus aureus and human THP-1 cells, parallel activity extracellular intracellular S. aureus. Moxifloxacin was used as a comparator. Delafloxacin showed MICs 3 5 log(2) dilutions lower than those moxifloxacin for collection...
Biofilms are associated with persistence of Staphylococcus aureus infections and therapeutic failures. Our aim was to set up a pharmacodynamic model comparing antibiotic activities against biofilms examining in parallel their effects on viability biofilm mass. S. ATCC 25923 (methicillin-sensitive [MSSA]) or 33591 (methicillin-resistant [MRSA]) were obtained by culture 96-well plates for 6 h/24 h. Antibiotic assessed after 24/48 h exposure concentrations ranging from 0.5 512 times the MIC....
ABSTRACT Biofilm-related infections remain a scourge. In an in vitro model of biofilms using Staphylococcus aureus reference strains, delafloxacin and daptomycin were found to be the most active among antibiotics from 8 different pharmacological classes (J. Bauer, W. Siala, P. M. Tulkens, F. Van Bambeke, Antimicrob. Agents Chemother. 57:2726–2737, 2013, doi:10.1128/AAC.00181-13). this study, we compared vancomycin produced by 7 clinical strains ( S. epidemic clones CC5 CC8) order rationalize...
The pharmacokinetic/pharmacodynamic index determining β-lactam activity is the percentage of dosing interval (%T) during which their free serum concentration remains above a critical threshold over minimum inhibitory (MIC). Regrettably, neither value %T nor that are clearly defined for critically-ill patients. Areas covered: We review and assess targets proposed β-lactams in illness by screening literature since 1997. Depending on study intention (clinical cure vs. suppression resistance),...
By their capacity to survive antibiotic pressure and regrow give rise a susceptible population once this is relieved, intracellular persisters of S. aureus may contribute explain therapeutic failures recurrent infections. Here, we show that the level dormancy subsequent resuscitate from resting state are dependent on oxidative stress in host cells where bacteria survive.
Using J774 macrophages, the intracellular activities of gentamicin, azithromycin, telithromycin, ciprofloxacin, moxifloxacin, and oritavancin (LY333328) against Staphylococcus aureus (strain ATCC 25923) have been quantitatively assessed in a 24-h model. S. was positively localized phagolysosomes by confocal electron microscopy, extracellular growth prevented with 0.5 mg gentamicin/liter (1x MIC) controls. When tested at concentrations equivalent to their maximum human serum, all antibiotics...
ABSTRACT The activities of ampicillin, meropenem, azithromycin, gentamicin, ciprofloxacin, and moxifloxacin against intracellular hemolysin-positive Listeria monocytogenes were measured in human THP-1 macrophages compared with the extracellular observed broth. All concentrations adjusted to explore ranges that are clinically achievable serum upon conventional therapy. In broth, azithromycin only bacteriostatic, whereas strongly bactericidal a concentration-dependent manner. cells,...