A5053922796- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Gut microbiota and health
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Colorectal Cancer Treatments and Studies
- Genomics and Phylogenetic Studies
- Cognitive Functions and Memory
- Mycobacterium research and diagnosis
- Aldose Reductase and Taurine
- Stress Responses and Cortisol
- Hypothalamic control of reproductive hormones
- Genital Health and Disease
- RNA Research and Splicing
- Anesthesia and Neurotoxicity Research
- Nail Diseases and Treatments
- Biochemical and Molecular Research
- Nicotinic Acetylcholine Receptors Study
- Immune Response and Inflammation
Ottawa Hospital
2010-2023
University of Ottawa
2008-2023
uOttawa Brain and Mind Research Institute
2018-2019
City University of New York
2015-2018
Ottawa Hospital Research Institute
2016-2018
Abstract The G/C single-nucleotide polymorphism in the serotonin 1a receptor promoter, rs6295, has previously been linked with depression, suicide and antidepressant responsiveness. In vitro studies suggest that rs6295 may have functional effects on expression of gene ( HTR1A ) through altered binding a number transcription factors. To further explore relationship between mental illness expression, we performed dual epidemiological biological studies. First, genotyped cohort 1412...
The serotonin-1A (5-HT1A) receptor is a key regulator of serotonergic activity and implicated in mood emotion. However, its post-transcriptional regulation has never been studied humans. In the present study, we show that "intronless" human 5-HT1A gene (HTR1A) alternatively spliced 3'-UTR, yielding two novel splice variants. These variants lack ∼1.6 kb intron, which contains an microRNA-135 (miR135) target site. Unlike HTR1A, mouse HTR1A lacks donor/accepter sites. Thus, splicing was not...
Abstract The 5-HT1A autoreceptor mediates feedback inhibition of serotonin (5-HT) neurons, and is implicated in major depression. human gene (HTR1A) rs6295 risk allele prevents Deaf1 binding to HTR1A, resulting increased transcription. Since chronic stress alters HTR1A methylation expression, we addressed whether recruitment methyl-binding protein MeCP2 may alter regulation at the locus. We show that enhances its site co-immunoprecipitates with cells brain tissue. Chromatin...
Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation-associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1)-dependent phosphorylation activation Interferon Regulatory Factor (IRF3) E3 ubiquitin ligase Pellino1, which are required interferon β (IFNβ) transcription. Here, we report that Pellino1 interacts with transcription factor Deformed Epidermal Autoregulatory (DEAF1). The interaction is independent activity but...
The C (-1019) G rs6295 promoter polymorphism of the serotonin-1A (5-HT1A) receptor gene is associated with major depression in several but not all studies, suggesting that compensatory mechanisms mediate resilience. risk allele prevents binding repressor Deaf1 increasing 5-HT1A transcription, and Deaf1-/- mouse model shows an increase autoreceptor expression. In this study, mice bred on a mixed C57BL6-BALB/c background were compared to wild-type littermates for function behavior males...
Serotonin (5-hydroxytryptamine, 5-HT) neurotransmission is negatively regulated by 5-HT1A autoreceptors on raphe neurons, and implicated in mood disorders. Pet-1/FEV an ETS transcription factor expressed exclusively serotonergic neurons essential for differentiation, although its regulation of 5-HT receptors has not yet been studied. Here, we show electrophoretic mobility shift assay that recombinant human binds directly to multiple Pet-1 elements the receptor promoter enhance...
Proofreading polymerases have 3' to 5' exonuclease activity that allows the excision and correction of mis-incorporated bases during DNA replication. In a previous study, we demonstrated in addition correcting substitution errors lowering error rate amplification, proofreading can also edit PCR primers match template sequences. Primer editing is feature be advantageous certain experimental contexts, such as amplicon-based microbiome profiling. Here develop set synthetic standards report on...
The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition functional state between individuals over time. Gene expression measurements can provide insights into microbiome function. However, efficient unbiased removal of microbial ribosomal RNA (rRNA) presents a barrier to acquiring metatranscriptomic data. Here we describe probe set achieves enzymatic rRNA complex human-associated communities. We demonstrate custom be further refined through an...
Serotonin 1A (5-HT1A) autoreceptors located on serotonin neurons inhibit their activity, and upregulation has been implicated in depression, suicide resistance to antidepressant treatment. Conversely, post-synaptic 5-HT1A heteroreceptors are important for response. The transcription factor deformed epidermal autoregulatory 1 (Deaf1) acts as a presynaptic repressor postsynaptic enhancer of transcription, but the mechanism is unclear. Because Deaf1 interacts with phosphorylated by glycogen...
Abstract Background The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition functional state between individuals over time. In studying function of microbiome mechanisms microbiota-mediated phenotypes, gene expression measurements provide additional insights to DNA-based composition. However, efficient unbiased removal microbial ribosomal RNA (rRNA) presents a barrier acquiring metatranscriptomic data, as rRNA typically accounts >90% total...
ABSTRACT Proofreading polymerases have 3’ to 5’ exonuclease activity that allows the excision and correction of mis-incorporated bases during DNA replication. In a previous study, we demonstrated in addition correcting substitution errors lowering error rate amplification, proofreading can also edit PCR primers match template sequences. Primer editing is feature be advantageous certain experimental contexts, such as amplicon-based microbiome profiling. Here develop set synthetic standards...