In this study, we analyzed the clinical significance of ferroptosis-related genes (FRGs) in 32 cancer types GSCA database.We detected a 2-82% mutation rate among 36 FRGs.In clear cell renal carcinoma (ccRCC; n=539) tissues from The Cancer Genome Atlas database, 30 FRGs were differentially expressed (up-or down-regulated) compared to normal kidney (n=72).Consensus clustering analysis identified two clusters based on similar co-expression ccRCC tissues.We then used LASSO regression build new...

Metabolic rearrangement is a marker of cancer that has been widely studied in recent years. One the major metabolic characteristics tumor cells high levels glycolysis, even under aerobic conditions, phenomenon called “Warburg effect.” We investigated expression and copy number variation (CNV) frequency all glycolysis‐related genes multiple types found many differentially expressed genes, particularly clear cell renal carcinoma (ccRCC). Single nucleotide variants (SNVs) showed overall average...

Abstract Growing evidence indicates that clear cell renal carcinoma (ccRCC) is a metabolism-related disease. Changes in fatty acid (FA) and cholesterol metabolism play important roles ccRCC development. As nuclear transcription factor receptor, Liver X receptor (LXR) regulates variety of key molecules associated with FA synthesis transport. Therefore, targeting LXR may provide new therapeutic targets for ccRCC. However, the potential regulatory effect molecular mechanisms remain unknown. In...

Chemoresistance is a major challenge in treating triple‑negative breast cancer (TNBC); chemotherapy remains the primary approach. The present study aimed to elucidate role of guanylate‑binding protein 2 (GBP2) activating autophagy TNBC and its impact on sensitivity cells paclitaxel (PTX). Transfection with lentivirus was performed establish cell lines stable, high GBP2 expression. mRNA levels expression were evaluated utilizing reverse transcription‑quantitative PCR western blotting,...

Bladder cancer (BLCA) is a common malignant cancer, and it the most genitourinary in world. The recurrence rate highest of all cancers, treatment BLCA has only slightly improved over past 30 years. Genetic environmental factors play an important role development progression BLCA. However, mechanism remains to be proven. Therefore, identification potential oncogenes urgent for developing new therapeutic directions designing novel biomarkers diagnosis prognosis Based on this need, we screened...

Berbamine (BBM), one of the bioactive ingredients extracted from Berberis plants, has attracted intensive attention because its significant antitumor activity against various malignancies. However, exact role and potential molecular mechanism berbamine in bladder cancer (BCa) remain unclear. In present study, our results showed that inhibited cell viability, colony formation, proliferation. Additionally, induced cycle arrest at S phase by a synergistic involving stimulation P21 P27 protein...

Targeted therapy for kidney cancer has achieved significant clinical results. However, because most patients who use targeted will develop drug resistance, we still need to constantly explore new therapeutic targets. Although porcupine (PORCN) as a palmitoyltransferase plays crucial role in the activation and secretion of Wnt proteins affects activity signaling pathway, little is known about PORCN clear cell renal carcinoma (ccRCC). We found that highly expressed lines with high expression...

Based on TCGA, GTEx, and TIMER databases various bioinformatics analysis methods, the potential biological roles of cuprotosis-related genes in pancreatic cancer were deeply explored, a predictive model for patients was constructed. We downloaded RNA-Seq data clinicopathological 179 tissues 332 adjacent normal from TCGA GTEx databases. The differential expression tissue analyzed, LASSO regression algorithm used to construct prediction verify validity prediction. algorithm, composed three...

Objective . This study is aimed at using genes related to the peroxisome proliferator-activated receptor (PPAR) pathway establish a prognostic risk model in kidney renal clear cell carcinoma (KIRC). Methods For this study, we first found PPAR pathway-related on gene set enrichment analysis (GSEA) website and KIRC mRNA expression data clinical through TCGA database. Subsequently, used R language multiple expansion packages analyze expression, hazard ratio analysis, coexpression of KIRC....

There is a need to improve diagnostic and therapeutic approaches enhance the prognosis of breast cancer, most common malignancy worldwide. Membrane lipid biosynthesis hot biological pathway in current cancer research. It unclear whether membrane involved BRCA. With LASSO regression, 14-gene prediction model was constructed using data from TCGA-BRCA cohort. The includes GPAA1, PIGF, ST3GAL1, ST6GALNAC4, PLPP2, ELOVL1, HACD1, SGPP1, PRKD2, VAPB, CERS2, SGMS2, ALDH3B2, HACD3. BRCA patients...

Abstract Mitochondrial function, as the core of cell's energy metabolism, is firmly connected to cancer metabolism and growth. However, involvement long noncoding RNAs (lncRNAs) related mitochondrial function in breast (BRCA) has not been thoroughly investigated. As a result, objective this research was dissect prognostic implication function-related lncRNAs their link immunological microenvironment BRCA. The Cancer Genome Atlas (TCGA) database used acquire clinicopathological transcriptome...

Ribophorin II (RPN2) is a highly conserved glycoprotein involved in the N-linked glycosylation of multiple proteins. RPN2 was reported to be associated with malignant phenotype several tumors. However, function bladder cancer (BCa) remains unclear.Expression BCa and adjacent tissues compared by bioinformatics analysis, immunohistochemistry, Western blotting. qRT-PCR performed explore correlation between expression various clinical features 38 patients. We assessed effects on biological...

This study is aimed at exploring the potential mechanism of PPAR signaling pathway in breast cancer (BRCA) and constructing a novel prognostic-related risk model. We used various bioinformatics methods databases to complete our exploration this research. Based on TCGA database, we use multiple extension packages based R language for data conversion, processing, statistics. LASSO regression analysis establish model BRCA. And combined online websites, including GEPIA, ImmuCellAI, TIMER, GDSC,...

This study aimed to explore underlying mechanisms by which sphingolipid-related genes play a role in kidney renal clear cell carcinoma (KIRC) and construct new prognosis-related risk model. We used variety of bioinformatics methods databases complete our exploration. Based on the TCGA database, we multiple R-based extension packages for data transformation, processing, statistical analyses. First, analyzing CNV, SNV, mRNA expression 29 various types cancers, found that vast majority were...

Abstract Background Long non‐coding RNAs (lncRNAs) regulate a variety of genes and biological processes. Lnc‐IL7R plays considerable role in the regulation inflammation, but its prognostic potential acute respiratory distress syndrome (ARDS) has not been fully explained. In this study, lnc‐IL7R as biomarker ARDS was examined. Objective Role ARDS. Methods LncRNA‐IL7R isolated from plasma patients with healthy controls clinical indexes were obtained within 24 h after admission. The relative...

Objective. Testicular germ cell tumors (TGCT) are a serious malignant tumor with low early diagnosis rates and high mortality. Methods. To investigate novel biomarkers to predict the prognosis of this cancer, bioinformatics analysis was used as an accurate, efficient, economical method. Results. Our study detected 39 upregulated 589 downregulated differentially expressed genes (DEGs) using GEO TCGA databases. identify function DEGs, GO functional analysis, three pathway (KEGG, REACTOME,...

This study aimed to explore the potential role of autophagy-related genes in kidney renal clear cell carcinoma (KIRC) and develop a new prognostic-related risk model. In our research, we used multiple bioinformatics methods perform pan-cancer analysis CNV, SNV, mRNA expression, overall survival genes, displayed results form heat maps. We then performed cluster LASSO regression on these KIRC. analysis, successfully divided patients with KIRC into five clusters found that there was correlation...

To characterize the clinicopathological features and evaluate neoadjuvant chemotherapy (NACT) efficacy of patients with human epidermal growth factor receptor 2 (HER2)-low breast cancer.A total 905 cancer who received 4 cycles thrice-weekly standard NACT in First Affiliated Hospital Chongqing Medical University were retrospectively enrolled, including 685 cases HER2-low expression 220 HER2-negative expression. Clinicopathological compared between Univariate multivariate logistic regression...

This research aims to identify the key fatty acid beta-oxidation (FAO) genes that are altered in kidney renal clear cell carcinoma (KIRC) and analyze role of these KIRC. The Gene Expression Omnibus (GEO) FAO datasets were used genes. Wilcoxon rank sum test was assess levels acyl-CoA dehydrogenase medium chain (ACADM) between KIRC non-cancer samples. logistic regression explore association ACADM clinical features. diagnostic performance for assessed using a receiver operating characteristic...

CDC6 is critical in DNA replication initiation, but its expression patterns and clinical implications cancer are underexplored. This study uses multi-omics data from The Cancer Genome Atlas (TCGA) to comprehensively analyze across various cancers, aiming evaluate potential as a prognostic biomarker explore role immunotherapy.

Purpose . To evaluate the expression of tripartite motif‐containing 33 (TRIM33) in ccRCC tissues and explore biological effect TRIM33 on progress ccRCC. Method The Cancer Genome Atlas (TCGA) database was used to examine mRNA levels its clinical relevance. Immunohistochemistry (IHC) performed obtained from our hospital. correlation between clinicopathological features patients also investigated. effects proliferation cells were examined using CCK‐8 colony formation assays. migration invasion...