- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Plant Disease Resistance and Genetics
- Yeasts and Rust Fungi Studies
- Bioenergy crop production and management
- Plant Pathogens and Resistance
- Plant and fungal interactions
- Plant and Fungal Interactions Research
- Plant pathogens and resistance mechanisms
- Isotope Analysis in Ecology
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Crop Yield and Soil Fertility
- Genetic and phenotypic traits in livestock
- Food Chemistry and Fat Analysis
- Species Distribution and Climate Change
- Metabolomics and Mass Spectrometry Studies
- Plant Disease Management Techniques
United States Department of Agriculture
2014-2024
Agricultural Research Service
2016-2024
University of Idaho
2020
Kenya Agricultural and Livestock Research Organization
2016
Cereal Disease Laboratory
2016
Edward T. Schafer Agricultural Research Center
2016
North Dakota State University
2016
Oregon State University
2012-2016
International Maize and Wheat Improvement Center
2016
University of California, Davis
2016
The recently identified Puccinia graminis f. sp. tritici (Pgt) race TTKSK (Ug99) poses a severe threat to global wheat production because of its broad virulence on several widely deployed resistance genes. Additional virulences have been detected in the Ug99 group races, and spread this has documented across growing regions Africa, Middle East (Yemen), West Asia (Iran). Other broadly virulent Pgt such as TRTTF TKTTF, present further difficulties maintaining abundant genetic for their...
ABSTRACT Accessions of common wheat ( Triticum aestivum L. subsp. ) from the USDA–ARS National Small Grains Collection (NSGC) are a resource for scientists worldwide. The genetic diversity core subset, representing approximately 10% collection's 42,138 T. accessions, was examined using 390 arrays technology (DArT) markers, 4941 single nucleotide polymorphisms (SNPs), and descriptor data. marker profiles revealed duplicates, which were excluded to form an informative (iCore) 3230 accessions....
Developing oat cultivars with partial resistance to crown rust would be beneficial and cost-effective for disease management. Two recombinant inbred-line populations were generated by crossing the susceptible cultivar Provena two partially resistant sources, CDC Boyer breeding line 94197A1-9-2-2-2-5. A third mapping population was sources validate quantitative trait locus (QTL) results. The three evaluated severity in field at Louisiana State University (LSU) 2009 2010 Cereal Disease...
Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a 'Lenetah' × 'Grannelose Zweizeilige' (GZ) cross. GZ is known major seedling QTL on chromosome 4H but linked markers suitable marker-assisted selection have not been developed. This study identified the (log of likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional chromosomes 6H (LOD 5.39 72.7 cM 4.24 34.46 cM, respectively). A 7H 2.04 81.07 cM) was suggested....
Wheat landrace PI 177906 has seedling resistance to stem rust caused by Puccinia graminis f. sp. tritici races TTKSK, TTKST, and BCCBC field the Ug99 race group. Parents, 140 recombinant inbred lines, 138 double haploid (DH) lines were evaluated for TTKSK BCCBC. Parents DH population in Kenya. The 90K wheat single nucleotide polymorphism (SNP) genotyping platform was used genotype parents populations. Goodness-of-fit tests indicated that two dominant genes conditioned TTKSK. Two major loci...
Sphaerellopsis filum is a mycoparasite of Puccinia graminis subsp. graminicola, rust fungus that causes widespread crop damage on perennial ryegrass grown for seed. In observations taken over the winter months, S. was found naturally colonizing 2% P. graminicola uredinia first-year plantings and 25% second-year ryegrass. controlled experiments conducted in glasshouses growth chambers, applied to rust-inoculated plants reduced lifetime spore production pustules by half, from 39,000 18,000...
Abstract Hemp ( Cannabis sativa L.) has historically played a vital role in agriculture across the globe. Feral and wild populations have served as genetic resources for breeding, conservation, adaptation to changing environmental conditions. However, feral of Cannabis, specifically Midwestern United States, remain poorly understood. This study aims characterize abiotic tolerances these populations, estimate suitable areas, identify regions at risk suitability change, highlight utility...
Dwarf bunt-resistant bread wheat accessions and SNP markers associated with DB resistance identified in this study are valuable resources for characterization deployment of wheat. bunt (DB), caused by Tilletia controversa J.G. Kühn, can significantly reduce grain yield quality on autumn-sown regions prolonged snow cover. be managed the use resistant cultivars. The objectives present were to characterize a large set from National Small Grains Collection genome-wide association approach...
Accessions of cultivated oat (Avena sativa L.) from the United States Department Agriculture-Agricultural Research Service Small Grains Collection in Aberdeen, ID were characterized for adult plant resistance (APR) and seedling to crown rust, caused by Puccinia coronata f. sp. avenae. Initially, 607 accessions with diverse geographic origins evaluated field tests Baton Rouge, LA. Of those, 97 not fully susceptible tested St. Paul, MN against a P. avenae population. Thirty-six that had some...
Abstract Key message Association mapping and phenotypic analysis of a diversity panel 238 bread wheat accessions highlights differences in resistance against common vs. dwarf bunt identifies genotypes valuable for bi-parental crosses. Common caused by Tilletia caries T. laevis was successfully controlled seed dressings with systemic fungicides decades, but has become renewed threat to yield quality organic agriculture where such treatments are forbidden. As the most efficient way address...
Wheat landrace CItr 15026 previously showed adult plant resistance (APR) to the Ug99 stem rust race group in Kenya and seedling Puccinia graminis f. sp. tritici races QFCSC, TTTTF, TRTTF. was crossed susceptible accessions LMPG-6 Red Bobs, 180 double haploid (DH) lines 140 recombinant inbred (RIL), respectively, were developed. The 90K wheat iSelect single-nucleotide polymorphism platform used genotype parents populations. Parents DH evaluated field for three seasons. A major quantitative...
Wheat breeders worldwide are seeking new sources of resistance to Puccinia graminis f. sp. tritici race TTKSK. To prioritize field-resistant landraces for follow-up genetic studies test the presence genes, seedling response P. TTKSK, molecular markers linked specific Sr segregation ratios among progeny from crosses, and bulked segregant analyses (BSA) were used. In total, 33 spring wheat with TTKSK crossed a susceptible genotype, LMPG-6. The stem rust reactions in F2 seedlings fit single...
Evaluation of oat crown rust resistance is usually based on visual assessment disease severity or infection types. Visual subjective, prone to rater bias and requires expert knowledge. PCR‐based quantitative assays can overcome challenges associated with assessment. New TaqMan primers probes were designed from Puccinia coronata f. sp. avenae (Pca) sequences. The primer–probe sets specific Pca, amplified using as little 0.5 pg fungal DNA (fDNA) allowed for scaling variation in sample total...
Abstract Barley ( Hordeum vulgare L.) ranks fourth in global cereal grain production and is an important crop for animal feed, malting, human consumption. Identification of two‐row barley germplasm with drought tolerance can increase genetic diversity facilitate future breeding efforts. The present study evaluated 480 spring accessions from the USDA National Small Grains Collection across two years irrigated terminal trials yield, test weight, protein content, thousand‐kernel kernel size....
Comparative sequence analysis was used to design a SNP marker that aided in the identification of new sources oat stem rust resistance. New races Puccinia graminis f. sp. avenae (Pga) threaten global production. An A. strigosa accession known carry broadly effective resistance gene, Pg6, crossed with two susceptible accessions generate 198 F2:3 families and 190 F5:6 RILs. The RIL population determine Pg6 single dominant gene located between 475 491 Mbp on diploid chromosome AA2 atlantica...
Oat crown rust, caused by Puccinia coronata Corda f. sp. avenae Eriks. ( Pca ), is a major biotic impediment to global oat production. Crown rust resistance has been described in diploid species A . strigosa accession PI 258731 and from this successfully introgressed into hexaploid sativa germplasm. The current study focuses on 1) mapping the location of QTL containing evaluating number quantitative trait loci (QTL) conditioning 258731; 2) understanding relationship between original genomic...
Oat crown rust caused by Puccinia coronata f. sp. avenae P . Syd & ( Pca ) is a major constraint to oat Avena sativa L.) production in many parts of the globe. The objectives this study were locate Pc96 on consensus map and develop SNP markers linked for use marker-assisted selection. loci resistance gene identified linkage analysis PACE assays developed selection breeding programs. race-specific originating from cultivated that has been deployed North American was mapped recombinant...
Wheat stem rust caused by Puccinia graminis f. sp. tritici is a widespread and recurring threat to wheat production. Emerging P. variants are rapidly overcoming major gene resistance deployed in cultivars new sources of race-nonspecific urgently needed. The National Small Grains Collection (NSGC) contains thousands landrace accessions that may harbor unique broadly effective emerging variants. All NSGC available facultative winter-habit bread landraces were tested field nursery St. Paul,...
In 2022, virus-like symptoms were observed in a field of diverse hemp (Cannabis sativa L.) germplasm Ontario County, New York. Less than 1% plants exhibited stunting and curled leaves (Figure S1), consistent with tobacco streak virus (TSV) on other (Liu et al. 2022). Most typically, the considerably reduced overall size, upwards, adaxial curling along leaf margin newer appearing to be most affected. Fifteen symptomatic representing nine accessions tested for 12 viruses viroids through Agdia...