A5063177038- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Colorectal and Anal Carcinomas
- Osteomyelitis and Bone Disorders Research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Anorectal Disease Treatments and Outcomes
- Cancer therapeutics and mechanisms
- Orthopedic Infections and Treatments
- Geochemistry and Elemental Analysis
- RNA Research and Splicing
- Urban Design and Spatial Analysis
- Diverticular Disease and Complications
- Biliary and Gastrointestinal Fistulas
- Pneumonia and Respiratory Infections
- Amyloidosis: Diagnosis, Treatment, Outcomes
- RNA regulation and disease
- Architecture, Modernity, and Design
- Paleontology and Stratigraphy of Fossils
- Cytomegalovirus and herpesvirus research
- Radiopharmaceutical Chemistry and Applications
AbbVie (United States)
2021
University of Wisconsin Carbone Cancer Center
2019
University of Wisconsin–Madison
2019
Rutgers, The State University of New Jersey
1981-2017
Rutgers New Jersey Medical School
2016-2017
Karolinska University Hospital
2016
Karolinska Institutet
2016
Gorgias Press (United States)
1994
Johnson University
1994
George Washington University
1990-1994
Fluoroquinolones and second-line injectable drugs are the backbone of treatment regimens for multidrug-resistant tuberculosis, resistance to these defines extensively drug-resistant tuberculosis. We assessed accuracy an automated, cartridge-based molecular assay detection, directly from sputum specimens, Mycobacterium tuberculosis with fluoroquinolones, aminoglycosides, isoniazid.
ABSTRACT Extensively drug-resistant (XDR) tuberculosis (TB) cannot be easily or quickly diagnosed. We developed a rapid, automated assay for the detection of XDR-TB plus resistance to drug isoniazid (INH) point-of-care use. Using simple filter-based cartridge with an integrated sample processing function, identified wide selection wild-type and mutant sequences associated directly from sputum. Four new large-Stokes-shift fluorophores were developed. When these four Stokes-shift combined six...
As indicated by its name, V-domain Ig suppressor of T cell activation (VISTA) is thought to serve primarily as an inhibitory protein that limits immune responses. VISTA antibodies can dampen the effects several concomitantly elicited signals, including TCR and TLR activation, but it currently unclear if agonism could singly affect biology. In this study, we discovered two novel characterized their on human peripheral blood mononuclear cells scRNA/CITE-seq. Both appeared agonize in...
Previous attempts to prepare monoclonal antibodies (MAbs) against S-antigen, a photoreceptor cell protein involved in the visual process and potent autoantigen for induction of experimental autoimmune uveitis (EAU), have yielded MAbs which define only carboxyl terminal epitopes. In this study we devised alternate strategies five directed other regions molecule. MAbC10C10 MAbH11-A2 were prepared synthetic peptides known be uveitopathogenic they selected more detailed studies. was generated...
Resistance to amikacin (AMK) and kanamycin (KAN) in clinical Mycobacterium tuberculosis strains is largely determined by specific mutations the rrs gene eis promoter. We developed a rapid, multiplexed sloppy molecular beacon (SMB) assay identify these then evaluated performance on 603 M. DNA samples collected South Korea. Assay was compared gold-standard phenotypic drug susceptibility tests, including Lowenstein-Jensen (LJ) absolute concentration, mycobacterial growth indicator tubes (MGIT),...
Troponin C has a 14-residue a-helix at the extreme amino terminus (the N-helix) which is absent in calmodulin.To learn significance of this region troponin C, residues 1-14 were deleted using site-directed mutagenesis.Analysis mutant (Al4-TnC) showed that deletion N-helix did not alter secondary structure C. Like wild type it exhibited Ca2+-dependent conformational changes based on electrophoretic mobility and increases content.The thermal stability A14- TnC, however, was 20 "C lower than...
Several molecular assays to detect resistance Rifampin, the Fluoroquinolones, and Aminoglycosides in Mycobacterium tuberculosis (M. tuberculosis) have been recently described. A systematic approach for comparing these laboratory is needed order determine relative advantage of each assay decide which ones should be advanced evaluation. We performed an analytic comparison a Sloppy Molecular Beacon (SMB) melting temperature (Tm) Dual labeled probe (DLP) Tm assay. Both targeted M. rpoB, gyrA,...
Abstract Previous studies have suggested that NK cells may limit T cell responses by their ability to eradicate dendritic cells, as demonstrated cell-mediated killing of generated from mouse bone marrow or human monocytes with GM-CSF. In the present study, we conventional in vitro Flt3 ligand spleens, were resistant lysis. However, upon stimulation GM-CSF, could mediate lysis these cells. GM-CSF-stimulated splenic increased surface expression costimulatory molecules and known ligands....
A study was performed to correlate activity quantitation derived from external imaging with surgical tumor specimens in patients who received radiolabeled monoclonal antibody. Patients were given I-131 labeled 16.88 human antibody and scanned 3-5 times by planar and/or single photon emission computed tomography methods acquire time-dependent data normal tissues. method also developed assess the heterogeneous distributions samples. Postsurgical tissue samples subdivided into volume elements...
ABSTRACT HIV-1 RNA genomes interact with diverse binding proteins (RBPs) in the cytoplasm including antiviral factor APOBEC3G (A3G) that, absence of viral Vif proteins, is packaged into virions. Where and when HIV-1-A3G interactions are initiated for packaging inside cell unknown, relative contributions genome vs. Gag capsid to this process remains controversial. Here we visualized A3G responses infection over an entire replication cycle using long-term (up 72 h) live single imaging. We show...