A5064048312- HIV Research and Treatment
- Cell Image Analysis Techniques
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- Microtubule and mitosis dynamics
- Genetics, Bioinformatics, and Biomedical Research
- Cellular transport and secretion
- Scientific Computing and Data Management
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Distributed and Parallel Computing Systems
- Cardiac and Coronary Surgery Techniques
- Wildlife Ecology and Conservation
- Image Processing Techniques and Applications
- Advanced Image and Video Retrieval Techniques
- Cancer-related gene regulation
- Cardiac, Anesthesia and Surgical Outcomes
- RNA regulation and disease
- Hepatitis B Virus Studies
- Bird parasitology and diseases
- Advanced Vision and Imaging
- Species Distribution and Climate Change
- Avian ecology and behavior
- Medical Image Segmentation Techniques
University of Wisconsin–Madison
2018-2025
Morgridge Institute for Research
2021-2024
University of Wisconsin Carbone Cancer Center
2018-2023
Optica
2021
Center for Cancer Research
2021
San Francisco State University
2014
Nature Research Centre
2013
Midwestern University
2005
Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to quantitative one. However, biologists are adopting these experiments become more complex, researchers need additional expertise carry out this work rigorous reproducible manner. This Essay provides navigational guide for experimental aid understanding of sample preparation through image acquisition, analysis, data interpretation. We discuss the interconnectedness...
Virion Infectivity Factor (Vif) of the Human Immunodeficiency Virus type 1 (HIV-1) targets and degrades cellular APOBEC3 proteins, key regulators intrinsic innate antiretroviral immune responses, thereby facilitating HIV-1 infection. While Vif’s role in degrading APOBEC3G is well-studied, Vif also known to cause cell cycle arrest, but detailed nature effects on has yet be delineated. In this study, we employed high-temporal single-cell live imaging super-resolution microscopy monitor...
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) genome diversification is a key determinant of viral evolution and the pathogenesis HIV/AIDS. Antiretroviral therapy non-curative, in context monitoring latent reservoir, precision tools are needed to detect enumerate HIV-1 genomes as well assess their heterogeneity, replication potential, predict responses therapy. Current sequencing-based methodologies often unable confirm intact most cell-based reporters provide limited information...
Virion Infectivity Factor (Vif) of the Human Immunodeficiency Virus type 1 (HIV-1) targets and degrades cellular APOBEC3 proteins, key regulators intrinsic innate antiretroviral immune responses, thereby facilitating HIV-1 infection. While Vif’s role in degrading APOBEC3G is well-studied, Vif also known to cause cell cycle arrest, but detailed nature effects on has yet be delineated. In this study, we employed high-temporal resolution single-cell live imaging super-resolution microscopy...
Open-source software tools are often used for analysis of scientific image data due to their flexibility and transparency in dealing with rapidly evolving imaging technologies. The complex nature problems frequently requires many be conjunction, including processing analysis, processing, machine learning deep learning, statistical the results, visualization, correlation heterogeneous but related data, more. However, development, therefore application, these computational is impeded by a lack...
HIV-1 generates unspliced (US), partially spliced (PS), and completely (CS) classes of RNAs, each playing distinct roles in viral replication. Elucidating their host protein 'interactomes' is crucial to understanding virus-host interplay. Here, we present HyPR-MSSV for isolation US, PS, CS transcripts from a single population infected CD4+ T-cells mass spectrometric identification vivo interactomes. Analysis revealed 212 proteins differentially associated with the unique RNA classes,...
Here we describe Haemoproteus (Haemoproteus) multivolutinus n. sp. from a tambourine dove (Turtur timpanistria) of Uganda and paramultipigmentatus (Haemosporida, Haemoproteidae) the Socorro common ground (Columbina passerina socorroensis) Island, Mexico. These parasites are described based on morphology their blood stages segments mitochondrial cytochrome b gene that can be used for molecular identification diagnosis these species. Gametocytes H. possess rod-like pigment granules evenly...
Cells derived from mice and other rodents exhibit profound blocks to HIV-1 virion production, reflecting species-specific incompatibilities between viral Tat Rev proteins essential host factors cyclin T1 (CCNT1) exportin-1 (XPO1, also known as CRM1), respectively. To determine if mouse cell than CCNT1 XPO1 affect HIV's postintegration stages, we studied HIV-1NL4-3 gene expression in NIH 3T3 cells modified constitutively express HIV-1-compatible versions of (3T3.CX cells). 3T3.CX supported...
Previously, we reported that cellular transcription factor ZASC1 facilitates DNA-dependent/RNA-independent recruitment of HIV-1 TAT and the elongation P-TEFb to promoter is a critical in regulating transcriptional (PLoS Path e1003712). Here report ZBTB2 novel repressor gene expression. strongly co-immunoprecipitated with was dramatically relocalized by from cytoplasm nucleus. Mutations abolishing ZASC1/ZBTB2 interaction prevented nuclear relocalization. We show ZBTB2-induced repression...
Hepatitis B virus (HBV) capsid assembly is traditionally thought to occur predominantly in the cytoplasm, where gains access virion egress pathway. To better define sites of HBV assembly, we carried out single cell imaging Core protein (Cp) subcellular trafficking over time under conditions supporting genome packaging and reverse transcription Huh7 hepatocellular carcinoma cells. Time-course analyses including live fluorescently tagged Cp derivatives showed accumulate nucleus at early points...
Abstract Virion Infectivity Factor (Vif) of the Human Immunodeficiency Virus type 1 (HIV-1) targets and degrades cellular APOBEC3 proteins, key regulators intrinsic innate antiretroviral immune responses, thereby facilitating HIV-1 infection. While Vif’s role in degrading APOBEC3G is well-studied, Vif also known to cause cell cycle arrest, but detailed nature effects on has yet be delineated. In this study, we employed high-temporal single-cell live imaging super-resolution microscopy...
Virion Infectivity Factor (Vif) of the Human Immunodeficiency Virus type 1 (HIV-1) targets and degrades cellular APOBEC3 proteins, key regulators intrinsic innate antiretroviral immune responses, thereby facilitating HIV-1 infection. While Vif’s role in degrading APOBEC3G is well-studied, Vif also known to cause cell cycle arrest, but detailed nature effects on has yet be delineated. In this study, we employed high-temporal resolution single-cell live imaging super-resolution microscopy...
Unlike humans, mice are unable to support HIV-1 infection. This is due, in part, a constellation of defined minor, species-specific differences conserved host proteins needed for viral gene expression. Here, we used precision CRISPR/Cas9 editing engineer "mousified" version one such protein, cyclin T1 (CCNT1), human T cells. CCNT1 essential efficient transcription, making it an intriguing target gene-based inactivation virus replication. We show that isogenic cell lines engineered encode...
In recent years, a steady decline in the number of perfusion education programs United States has been noted. At same time, there parallel students graduated from educational States. Also, as noted by several authors, an increase demand for graduates. The and graduates also anesthesia surgical residency programs. shift is caused combination economic clinical factors. First, decreased reimbursement led to reallocation hospital resources. Second, original enthusiasm beating heart coronary...
Abstract ImageJ provides a framework for image processing across scientific domains while being fully open source. Over the years has been substantially extended to support novel applications in imaging as they emerge, particularly area of biological microscopy, with functionality made more accessible via Fiji distribution ImageJ. Within this software ecosystem, work done extend accessibility utilize scripting, macros, and plugins variety programming scenarios, e.g., from Groovy Python...
Many scientific software platforms provide plugin mechanisms that simplify the integration, deployment, and execution of externally developed functionality. One most widely used in imaging space is Fiji, a popular open-source application for image analysis. Fiji incorporates builds on ImageJ ImageJ2 platforms, which powerful architecture by thousands plugins to solve wide variety problems. This capability major part Fiji's success, it has become biological analysis tool target new However,...
Deep learning has greatly accelerated research in biological image analysis yet it often requires programming skills and specialized tool installation. Here we present Piximi, a modern, no-programming leveraging deep learning. Implemented as web application at Piximi.app, Piximi no installation can be accessed by any modern browser. Its client-only architecture preserves the security of researcher data running all computation locally. offers four core modules: classifier, an annotator,...
Decades of iteration on scientific imaging hardware and software has yielded an explosion in not only the size, complexity, heterogeneity image datasets but also tooling used to analyze this data. This wealth analysis tools, spanning different programming languages, frameworks, data structures, is itself a problem for analysts who must adapt new technologies integrate established routines solve increasingly complex problems. While many “bridge” layers exist unify pairs popular there exists...
The human immunodeficiency virus type 1 (HIV-1) Virion Infectivity Factor (Vif) targets and degrades cellular APOBEC3 proteins, key regulators of intrinsic innate antiretroviral immune responses, thereby facilitating HIV-1 infection. While Vif’s role in degrading APOBEC3G is well-studied, Vif also known to cause cell cycle arrest but the detailed nature effects on has yet be delineated. In this study, we employed high-temporal single-cell live imaging super-resolution microscopy monitor...