A5061807748- Methane Hydrates and Related Phenomena
- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Ionosphere and magnetosphere dynamics
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Plant Virus Research Studies
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- RNA and protein synthesis mechanisms
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis Viruses Studies and Epidemiology
- Superconducting Materials and Applications
University of Wisconsin–Madison
2014-2023
Morgridge Institute for Research
2020-2023
University of Wisconsin Carbone Cancer Center
2023
Assembly Biosciences (United States)
2022
ABSTRACT Hepatitis B virus (HBV) capsid proteins (Cps) assemble around the pregenomic RNA (pgRNA) and viral reverse transcriptase (P). pgRNA is then transcribed to double-stranded DNA (dsDNA) within capsid. The Cp assembly domain, which forms shell of capsid, regulates kinetics stability. Cp, via its nucleic acid-binding C-terminal also affects acid organization. We hypothesize that structure may have a direct effect on processing. Using structure-guided design, we made series mutations at...
Significance Positive-strand RNA [(+)RNA] viruses constitute the largest genetic class of and include many high-impact pathogens, such as SARS–CoV-2 (COVID-19 pandemic coronavirus), MERS CoV, Zika, chikungunya, dengue, hepatitis C viruses. (+)RNA virus genome replication invariably occurs on virus-induced, membrane-bound organelles called complexes, an attractive potential target for broadly active antivirals. To better understand, control beneficially employ viruses, urgent needs exist to...
Positive-strand RNA viruses replicate their genomes in virus-induced membrane vesicles, and the resulting replication complexes are a major target for virus control. Nodavirus studies first revealed viral proteins forming 12-fold symmetric “crown” at vesicle opening to cytosol, an arrangement recently confirmed extend distantly related alphaviruses. Using cryoelectron microscopy (cryo-EM), we show that mature nodavirus crowns comprise two stacked 12-mer rings of multidomain protein A. Each...
Hepatitis B virus (HBV) capsid assembly is traditionally thought to occur predominantly in the cytoplasm, where gains access virion egress pathway. To better define sites of HBV assembly, we carried out single cell imaging Core protein (Cp) subcellular trafficking over time under conditions supporting genome packaging and reverse transcription Huh7 hepatocellular carcinoma cells. Time-course analyses including live fluorescently tagged Cp derivatives showed accumulate nucleus at early points...
ABSTRACT To understand subcellular sites of hepatitis B virus (HBV) replication, we visualized core (Cp), polymerase (Pol), and pregenomic RNA (pgRNA) in infected cells. Interestingly, found that the majority Pol localized to mitochondria cells undergoing viral replication. The mitochondrial localization was independent both cell type other components, indicating contains an intrinsic targeting signal (MTS). Neither Cp nor pgRNA during active suggesting a role than DNA synthesis for at...
Positive-strand RNA virus genome replication occurs in membrane-associated complexes (RCs). Nodavirus RCs are outer mitochondrial membrane invaginations whose necked openings to the cytosol "crowned" by a 12-fold symmetrical proteinaceous ring that functions as main engine of replication. Similar protein crowns recently visualized at alphavirus and coronavirus highlight their broad conservation functional importance. Using cryo-EM tomography, we earlier showed major nodavirus crown...
Abstract Positive-strand RNA viruses replicate their genomes in virus-induced membrane vesicles, and the resulting replication complexes are a major target for virus control. Nodavirus studies first revealed viral proteins forming 12-fold symmetric “crown” at vesicle opening to cytosol, an arrangement recently confirmed extend distantly related alphaviruses. Using cryo-electron microscopy (cryo-EM), we show that mature nodavirus crowns comprise two stacked 12-mer rings of multi-domain...
Journal Article Single Particle Cryo-EM and Cryo-Tomography Resolve Nodavirus RNA Replication Crown Assembly Get access Hong Zhan, Zhan Rowe Center for Virology, Morgridge Institute Research, Madison, WI, United StatesUniversity of Wisconsin-Madison, States Search other works by this author on: Oxford Academic Google Scholar Nuruddin Unchwaniwala, Unchwaniwala Andrea Rebolledo-Viveros, Rebolledo-Viveros Janice Pennington, Pennington University Mark Horswill, Horswill Roma Broadberry,...