A5038890159- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Plant pathogens and resistance mechanisms
- Aquaculture disease management and microbiota
- Agronomic Practices and Intercropping Systems
- CAR-T cell therapy research
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- Plant Pathogens and Resistance
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- Ruminant Nutrition and Digestive Physiology
- Renal and related cancers
- Abdominal vascular conditions and treatments
Virginia Commonwealth University
2024
Sunnybrook Health Science Centre
2014-2023
University of Toronto
2014-2023
Sunnybrook Research Institute
2010-2022
Sunnybrook Hospital
2016-2022
University of Minnesota
1986-2018
Georgetown University
2017
Michigan Medicine
2016
Centre for Social Innovation
2016
Health Sciences Centre
2004-2010
Ets family transcription factors control the expression of a large number genes in hematopoietic cells. Here we show strikingly precise differential subset these marking critical, early stages mouse lymphocyte cell-type specification. Initially, member factor Erg was identified during an arrayed cDNA library screen for encoding expressed specifically T cell lineage commitment. Multiparameter fluorescence-activated sorting over dozen surface markers used to isolate 18 distinct primary-cell...
Abstract T cells are pivotal effectors of the immune system and can be harnessed as therapeutics for regenerative medicine cancer immunotherapy. An unmet challenge in field is development a clinically relevant that readily scalable to generate large numbers T-lineage from hematopoietic stem/progenitor (HSPCs). Here, we report stromal cell-free, microbead-based approach supports efficient vitro both human progenitor (proT) CD34 + sourced cord blood, GCSF-mobilized peripheral pluripotent stem...
The B1 gene of vaccinia virus encodes a 34-kDa protein which is essential for viral replication.Temperature-sensitive mutants bearing lesions in this arrest at the stage DNA replication during nonpermissive infections.In report, sequence open reading frame presented, and mutations two ts alleles are identified.Analysis deduced reveals strong homology with catalytic domains numerous kinases.The lesion one alters an invariant glycine residue within such domain.
Vaccinia virus mutants ts2 and ts25, members of the same complementation group, exhibit a temperature-dependent arrest at stage viral DNA replication. The lesions responsible for mutant phenotypes have been localized to far left region HindIII B genomic fragment by marker rescue studies. Hybrid selection analyses established that fragments positive represented first open reading frame encoded 30-kilodalton protein. gene is expressed early after infection as rightwardly transcribed...
IL-17-producing γδ T (γδT17) cells are critical components of the innate immune system. However, gene networks that control their development unclear. Here we show HEB (HeLa E-box binding protein, encoded by Tcf12) is required for generation a newly defined subset fetal-derived CD73- γδT17 cells. in immature CD24+CD73- expression Sox4, Sox13, and Rorc, these genes repressed acute antagonist Id3. HEB-deficiency also affects mature CD73+ cells, which defective RORγt IL-17 production....
Cartilaginous fish express canonical B and T cell recognition genes, but their lymphoid organs lymphocyte development have been poorly defined. Here, the expression of Ig, TCR, recombination-activating gene (Rag)-1 terminal deoxynucleosidase (TdT) genes has used to identify roles various tissues throughout in cartilaginous fish, Raja eglanteria (clearnose skate). In embryogenesis, Ig TCR are sharply up-regulated at 8 weeks development. At this stage TdT is limited thymus; later, appears...
The basic helix-loop-helix (bHLH) transcription factors HEB and E2A are critical mediators of gene regulation during lymphocyte development. We have cloned a new factor, called HEBAlt, from pro-T cell cDNA library. HEBAlt is generated by alternative transcriptional initiation splicing the locus, which also encodes previously characterized E box protein HEBCan. contains unique N-terminal coding exon (the Alt domain) that replaces first transactivation domain Downstream domain, identical to...
Abstract The runt family transcription factors Runx1 and Runx3 are expressed in developing murine thymocytes. We show that enforced expression of full-length CD4−CD8− thymocytes results a profound suppression immature CD4/CD8 double-positive mature CD4 single-positive compared with controls. This effect arises from Runx1- or Runx3-mediated repression expression, is independent positively selecting signals. able to repress thymocytes, but not splenic T cells. Runx-mediated association the...
In all vertebrate species examined to date, rearrangement and somatic modification of gene segmental elements that encode portions the antigen-combining sites immunoglobulins are integral components generation antibody diversity. phylogenetically primitive cartilaginous fishes, segments encoding immunoglobulin heavy light chain loci arranged in multiple clusters, which separated by only 300-400 bp. some cases, joined germline nonlymphoid cells (joined genes). Both genomic library screening...
To determine the effect of nitrous oxide on chromosome doubling in diploid red dover ( Trifolium pratense L.), excised heads were treated at 6 bars atmospheric pressure. Treatment commenced2 4 hours after crossing and continued for following 24 hours. Of plants which flowered, 71% classified as tetraploid based pollen size. Subsequent root‐tip PMC counts 136 these revealed that 119 tetraploid, 3 diploid, 12 aneuploid. Two died before made. The technique produced 49 79% tetraploids T....
Biofilms complicate treatment of Staphylococcus aureus (SA) wound infections. Previously, we determined alpha-toxin (AT)-promoted SA biofilm formation on mucosal tissue. Therefore, evaluated isolates for AT production and epithelium assessed the role in formation. Thirty-eight were molecularly typed by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (ST), spa typing. We measured these vitro ex vivo quantified production. also investigated effect an anti-AT monoclonal...
Induced pluripotent stem cells (iPSC) derived from healthy individuals are important controls for disease-modeling studies. Here we apply precision health to create a high-quality resource of control iPSCs. Footprint-free lines were reprogrammed four volunteers the Personal Genome Project Canada (PGPC). Multilineage-directed differentiation efficiently produced functional cortical neurons, cardiomyocytes and hepatocytes. Pilot users demonstrated versatility by generating kidney organoids, T...