A5083313087- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Molecular Biology Techniques and Applications
- Ovarian function and disorders
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive Health and Technologies
- Cancer-related molecular mechanisms research
- Genomic variations and chromosomal abnormalities
- Biomarkers in Disease Mechanisms
- Viral gastroenteritis research and epidemiology
- Effects of Environmental Stressors on Livestock
- Cell Adhesion Molecules Research
- Seed Germination and Physiology
- Genetics, Aging, and Longevity in Model Organisms
- Evolutionary Game Theory and Cooperation
- Ultrasound Imaging and Elastography
- RNA Interference and Gene Delivery
John Radcliffe Hospital
2021-2024
University of Oxford
2021-2024
University of Antwerp
2023-2024
University of Liège
2021
National Agriculture and Food Research Organization
2016-2018
Institute of Agrobiological Sciences
2016-2018
Ghent University
2013-2015
Clinical mastitis (CM) is an inflammatory disease occurring in the mammary glands of lactating cows. CM under genetic control, and a prominent resistance QTL located on chromosome 6 was reported various dairy cattle breeds. Nevertheless, biological mechanism underpinning this has been lacking. Herein, we mapped, fine-mapped, discovered putative causal variant underlying Dutch population. We identified ~12 kb multi-allelic copy number (CNV), that perfect linkage disequilibrium with lead SNP,...
Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, rate of embryo development remains low. In present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP glutathione (GSH) levels were compared between cumulus-oocyte complexes (COCs) control COCs (no cryoprotectant treatment no cooling), as well a toxicity cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol propylene glycol....
We tested the effects of resveratrol both as a pre-treatment and recovery treatment after warming during in vitro maturation (IVM) on viability developmental competence porcine oocytes vitrified at germinal vesicle stage. Pre-treatment before vitrification for 3 hr with 2 μM did not affect survival, oocyte embryo to blastocyst stage parthenogenetic activation. However, supplementation medium subsequent IVM significantly improved ability surviving develop stage, this effect was observed only...
Can ovarian tissue morphology be better preserved whilst enabling histological molecular analyses following fixation with a novel fixative, neutral buffered formalin (NBF) 5% acetic acid (referred to hereafter as Form-Acetic)?Fixation Form-Acetic improved histology compared NBF in multiple species while still analyses.NBF results shrinkage various types including the ovary. Components of tissue, notably follicles, are particularly susceptible NBF-induced morphological alterations and can...
Abstract Co‐culture of cumulus‐oocyte complexes (COCs) with denuded oocytes (DOs) during in vitro maturation (IVM) was reported to improve the developmental competence via oocyte‐secreted factors cattle. The aim present study investigate if addition DOs IVM can fertilization (IVF) and culture (IVC) results for a defined production system pigs. medium porcine oocyte supplemented gonadotropins, dbcAMP β‐mercaptoethanol. Cumulus‐oocyte were matured without or different ratios (9 COC, 9 COC+16...
We evaluated the effects of polyethylene glycol (PEG) and Supercool X-1000 (SC) as supplements during vitrification immature cumulus-enclosed porcine oocytes in a solution based on 17.5% ethylene + propylene glycol. After warming, were subjected to vitro maturation, fertilization embryo culture. In Experiment 1, equilibration solutions supplemented with or without 2% (w/v) PEG (PEG+ PEG-, respectively). The survival rate, cleavage blastocyst development similar between PEG+ PEG- groups;...
Female rhinoceroses have an ovarian reserve that consists of immature oocytes in primordial follicles. Establishing methods to grow and culture these from those follicles the laboratory might fuel efforts towards vitro embryo production this species without need for repeated vivo oocyte collection. In depth understanding biology behind improvement assisted reproductive techniques (ARTs) is only option preventing disappearance functionally extinct such as northern white rhinoceros. follicle...
The northern white rhinoceros is functionally extinct with only two females left. Establishing methods to culture ovarian tissues, follicles, and oocytes generate eggs will support conservation efforts using in vitro embryo production. To the best of our knowledge, this first description structure molecular signature any rhinoceros, more specifically, we describe neonatal adult southern (Ceratotherium simum simum) ovary; closest relation rhinoceros. Interestingly, all ovaries contain...
In the present study, we propose an alternative technique called cytoplast fusion to improve maturation rate and developmental competence of growing oocytes collected from early antral follicles in pigs. We examined whether a oocyte with fully-grown (CFR group) could better promote compared germinal vesicle (GV) transfer (GVTR group). After 44 h vitro (IVM), most (GR were still arrested at GV stage (64.0 ± 5.1%); this number was significantly higher (P < 0.01) than that other groups. No...
Abstract In vitro maturation (IVM) in serum causes hampered expansion of porcine cumulus‐oocyte complexes (COCs) due to excessive alpha 2 ‐macroglobulin (A2M). This study investigated two hypotheses that could explain the effect A2M: (i) binding epidermal growth factor (EGF) A2M, followed by its decreased availability; and (ii) inhibition zinc‐dependent metalloproteases. Cumulus was evaluated based on diameter COCs, proportion COCs participating a floating cloud with loss cumulus cells. The...
Mitochondrial quality is implicated as a contributor to declining fertility with aging. We investigated mitochondrial transcripts in oocytes and their associated cumulus cells from mice of different ages using RNA-seq. Mice aged 3 weeks, 9 1 year were superovulated, 48 h later, oocyte complexes collected by follicle puncture. did not detect any major differences that could be attributed However, RNA which deviated the consensus sequence found at higher frequency than corresponding oocyte....