A5091727794- ATP Synthase and ATPases Research
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Molecular Research
- MicroRNA in disease regulation
- Inflammatory mediators and NSAID effects
- Cellular transport and secretion
- Computational Drug Discovery Methods
- Extracellular vesicles in disease
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Machine Learning in Bioinformatics
- Advanced Fluorescence Microscopy Techniques
- Estrogen and related hormone effects
- Heat shock proteins research
- RNA modifications and cancer
- Protein purification and stability
- Lipid Membrane Structure and Behavior
Max Planck Institute of Molecular Plant Physiology
2024-2025
Universidad Nacional Autónoma de México
2018-2022
Exosomes can transport regulatory biomolecules and are mediators of cellular signaling among metabolic tissues through endocrine mechanisms. Understanding the pathways processes underlying exosome-mediated inter-tissue communication is critical for elucidating molecular pathophysiology diseases such as obesity, type 2 diabetes mellitus (T2DM), cardiovascular disorders. Consequently, these mechanisms represent novel promising targets pharmacological regulation. We examined current knowledge...
With the uncontrolled growth of multidrug-resistant bacteria, there is an urgent need to search for new therapeutic targets, develop drugs with novel modes bactericidal action. FoF1-ATP synthase plays a crucial role in bacterial bioenergetic processes, and it has emerged as attractive antimicrobial target, validated by pharmaceutical approval inhibitor treat tuberculosis. In this work, we aimed design, through two types silico strategies, allosteric inhibitors ATP synthase, targeting...
In addition to playing a central role in the mitochondria as main producer of ATP, FOF1-ATP synthase performs diverse key regulatory functions cell membrane. Its malfunction has been linked growing number human diseases, including hypertension, atherosclerosis, cancer, and some neurodegenerative, autoimmune, aging diseases. Furthermore, inhibition this enzyme jeopardizes survival several bacterial pathogens public health concern. Therefore, emerged novel drug target both treat diseases...
Amphiphysin 2 and members of the BAR-domain family proteins participate in a wide array cellular processes including cell cycle endocytosis. Given that amphiphysin is related to diverse responses as result metabolic stress, we investigated macrophages whether oxidative stress originated by internalization oxidized low density lipoproteins (oxLDL) affect both, expression its binding partner c-Myc. Here report under complex formation between 2(Bin1) c-Myc allows develop novel survival...
The temperature upshift has been widely used as an induction system to produce recombinant proteins (RPs). However, thermoinduction could affect bacterial metabolism, RP production, and aggregation. Understanding the structure functionality of those aggregates, known inclusion bodies (IBs), is a research area interest in bioprocesses being scarcely studied under thermoinduction. Here, we describe effect (39°C or 42°C) on production human granulocyte–macrophage colony-stimulating factor...
Approximately 80% of breast cancer (BC) cases are estrogen receptor positive (ER+) and sensitive to hormone treatment; Tamoxifen is a prodrug, its main plasmatic active metabolites 4-hydroxytamoxifen (4-OH Tam) endoxifen. Despite the effectiveness tamoxifen therapy, resistance can be developed. An increment in eukaryotic initiation factor-4A complex (eIF4A) activity result tamoxifen-resistant tumor cells. For this work, we developed cell variant resistant 4-OH Tam endoxifen, denominated...
Enzyme subunit interfaces have remarkable potential in drug design as both target and scaffold for their own inhibitors. We show an evolution-driven strategy the de novo of peptide inhibitors targeting Escherichia coli FoF1-ATP synthase a case study. The evolutionary algorithm ROSE was applied to generate diversity-oriented libraries by engineering fragments from ATP interfaces. resulting peptides were scored with PPI-Detect, sequence-based predictor protein-protein interactions. Two...
Enzyme's subunits interfaces have remarkable potential in drug design as both target and scaffold for their own inhibitors. We show an evolution-driven strategy the de novo of peptide inhibitors targeting E. coli's FoF1-ATP synthase a case study. The evolutionary algorithm ROSE is applied to generate diversity-oriented libraries by engineering fragments from ATP interfaces. resulting peptides are scored with PPI-Detect, sequence-based predictor protein-protein interactions. Two selected were...