A5017467650- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Oral microbiology and periodontitis research
- Probiotics and Fermented Foods
- Bacterial Infections and Vaccines
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Peptides and Activities
- Galectins and Cancer Biology
- Parasitic Infections and Diagnostics
- Actinomycetales infections and treatment
- Extracellular vesicles in disease
- Digital Imaging in Medicine
- Adenosine and Purinergic Signaling
- Advanced Drug Delivery Systems
- Reproductive tract infections research
- Infectious Diseases and Mycology
- Polysaccharides and Plant Cell Walls
- E-Learning and Knowledge Management
- Fungal and yeast genetics research
- Educational Technology in Learning
Universidad Complutense de Madrid
2016-2021
Instituto Ramón y Cajal de Investigación Sanitaria
2016-2019
University of Minho
2011-2017
Candida albicans cell wall is important for growth and interaction with the environment. RLM1 one of putative transcription factors involved in integrity pathway, which plays an role maintenance integrity. In this work we investigated involvement biogenesis virulence. Newly constructed C. Δ/Δrlm1 mutants showed typical weakening phenotypes, such as hypersensitivity to Congo Red, Calcofluor White, caspofungin (phenotype reverted presence sorbitol), confirming Additionally, a significant...
The effectiveness of macrophages in the response to systemic candidiasis is crucial an effective clearance pathogen. secretion proteins, mRNAs, noncoding RNAs and lipids through extracellular vesicles (EVs) one mechanisms communication between immune cells. EVs change their cargo mediate different responses, may play a role against infections. Thus we have undertaken first quantitative proteomic analysis on protein composition THP-1 macrophage-derived during interaction with Candida...
Nosocomial fungal bloodstream infections (BSI) are increasing significantly in hospitalized patients and Candida parapsilosis has emerged as an important pathogen responsible for numerous outbreaks. The objective of this study was to evaluate C. sensu lato infection scenarios, regarding species distribution strain relatedness. One hundred isolates derived from blood cultures catheter tips were analysed by multiplex microsatellite typing sequencing D1/D2 regions the ribosomal DNA. Our results...
Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters a predisposing factor for IC. Hyphal forms Candida albicans (the major etiological agent IC) are related to invasion host tissues. The secreted proteins hyphae involved virulence, interaction, immune response, evasion. To identify IC diagnostic biomarker candidates, we characterized the C. hyphal secretome by gel-free proteomic...
Abstract The absence of high-affinity potassium uptake in Candida glabrata , the consequence deletion TRK1 gene encoding sole potassium-specific transporter, has a pleiotropic effect. Here, we show that addition to changes basic physiological parameters (e.g., membrane potential and intracellular pH) decreased tolerance various cell stresses, loss high affinity also alters cell-surface properties, such as an increased hydrophobicity adherence capacity. efficient system results diminished...
The secreted aspartic proteases (Saps) are among the most studied virulence determinants in Candida albicans. These proteins translated as pre-pro-enzymes consisting of a signal sequence followed by propeptide and mature enzyme. propeptides proteinases important for correct processing, folding/secretion In this study, DNA sequences C. albicans Saps were screened microsatellite was identified SAP8 region. genetic variability repetitive region Sap8 determined 108 independent strains isolated...
Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters a predisposing factor for IC. Hyphal forms Candida albicans (the major etiological agent IC) are related to invasion host tissues. The secreted proteins hyphae involved virulence, interaction, immune response, evasion. To identify IC diagnostic biomarker candidates, we characterized the C. hyphal secretome by gel-free proteomic...
Macrophages are involved in the primary human response to Candida albicans. After pathogen recognition, signaling pathways activated, leading production of cytokines, chemokines, and antimicrobial peptides. ATP binding proteins crucial for this regulation. Here, a quantitative proteomic phosphoproteomic approach was carried out study macrophage ATP-binding after interaction with C. From total 547 nonredundant quantified proteins, 137 were 59 detected as differentially abundant. abundant 6...
Previous studies have correlated the severity of recurrent vulvovaginal Candida infections (VVC) and balanitis in patients from China with presence some dominant genotypes at ORF RLM1. Here we tested VVC vs non-VVC isolates Portugal, Brazil Greece and, although same were identified isolates, they present only five out 150 strains. However, this analysis showed that presented a higher percentage similar high molecular weight alleles, comparison strains isolated other biological sources.
The transition between yeast and hyphal morphologies plays a crucial role in the pathogenicity of Candida albicans . Recent studies have pointed out great relevance extracellular vesicles (EVs) secreted by microorganisms wide variety biological processes including interaction with host. Therefore, main objective this work was to compare EVs forms shed light on C. -host interaction. were obtained ultracentrifugation culture medium supernatant analysed mass spectrometry. They characterized...