A5051367662- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- CAR-T cell therapy research
- Integrated Circuits and Semiconductor Failure Analysis
- SARS-CoV-2 and COVID-19 Research
- Advancements in Semiconductor Devices and Circuit Design
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Polymer Surface Interaction Studies
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- Biopolymer Synthesis and Applications
- Membrane Separation Technologies
- Reproductive System and Pregnancy
- Advanced Polymer Synthesis and Characterization
- Electrospun Nanofibers in Biomedical Applications
- Dendrimers and Hyperbranched Polymers
University of Pennsylvania
2021-2023
Polytechnique Montréal
2017-2022
California University of Pennsylvania
2021
Fibrosis affects millions of people with cardiac disease. We developed a therapeutic approach to generate transient antifibrotic chimeric antigen receptor (CAR) T cells in vivo by delivering modified messenger RNA (mRNA) cell–targeted lipid nanoparticles (LNPs). The efficacy these vivo–reprogrammed CAR was evaluated injecting CD5-targeted LNPs into mouse model heart failure. Efficient delivery mRNA encoding the lymphocytes observed, which produced transient, effective vivo. Antifibrotic...
Abstract Lipid Nanoparticles (LNPs) are used to deliver siRNA and COVID-19 mRNA vaccines. The main factor known determine their delivery efficiency is the pKa of LNP containing an ionizable lipid. Herein, we report a method that can predict from structure We theoretical, NMR, fluorescent-dye binding, electrophoretic mobility methods comprehensively measure protonation both lipid formulated LNP. was 2-3 units higher than primarily due proton solvation energy differences between aqueous...
<title>Abstract</title> Concerns with current mRNA Lipid Nanoparticle (LNP) systems include dose-limiting reactogenicity, adverse events that may be partly due to systemic off target expression of the immunogen, and a very limited understanding mechanisms responsible for frozen storage requirement. We applied new rational design process identify novel multiprotic ionizable lipid, called C24, as key component LNP delivery system. show resulting C24 has multistage protonation behavior in...