Maxsueli Aparecida Moura Machado

ORCID: 0000-0002-0189-9017
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Research Areas
  • Listeria monocytogenes in Food Safety
  • Food Safety and Hygiene
  • Salmonella and Campylobacter epidemiology
  • Escherichia coli research studies
  • Meat and Animal Product Quality
  • Microbial Inactivation Methods
  • Vibrio bacteria research studies
  • Advanced Chemical Sensor Technologies
  • Antibiotic Resistance in Bacteria
  • Essential Oils and Antimicrobial Activity
  • Aquaculture disease management and microbiota
  • Spectroscopy and Chemometric Analyses
  • Probiotics and Fermented Foods
  • Bee Products Chemical Analysis
  • Spectroscopy Techniques in Biomedical and Chemical Research
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Identification and Quantification in Food
  • Food Waste Reduction and Sustainability
  • Genomics and Phylogenetic Studies
  • Bacteriophages and microbial interactions
  • Viral gastroenteritis research and epidemiology
  • Insect Pest Control Strategies
  • Agricultural and Food Sciences
  • Heat shock proteins research

Universidade Federal do Rio de Janeiro
2022-2025

Universidade Federal de Mato Grosso
2018-2023

Escherichia coli (E. coli) carrying the transmissible locus of stress tolerance (tLST) are able to overcome numerous environmental challenges. In our in-silico study, we aimed characterize tLST in terms its variants 793 genomes E. from Brazil originating food, and clinical (animal human) sources, perform a temporal analysis order identify historical moment emergence. We also analyzed presence two Yersinia high pathogenicity island (HPI) genomes, describing other genes accessory for...

10.1038/s41598-025-89066-1 article EN cc-by-nc-nd Scientific Reports 2025-02-11

This study aimed to obtain and characterize an oil-in-water nanoemulsion (NE) loaded with in vitro optimized bactericidal essential oil blend of 50% oregano, 40% thyme, 10% lemongrass evaluate its potential at three different concentrations (0.5%, 1%, 2%) the inactivation Escherichia coli, Staphylococcus aureus, Salmonella enterica serotype Enteritidis inoculated rainbow trout fillets stored 4 °C for 9 days. Regarding NE, nanometric size (<100 nm) low polydispersion (0.17 ± 0.02) was...

10.3390/foods13101569 article EN cc-by Foods 2024-05-17

Fish is a nutritionally rich product; however, it easily contaminated by pathogenic microorganisms, such as Salmonella spp. Therefore, this study aimed to identify the best concentration of sodium hypochlorite (NaClO), exposure time, and water temperature that allow most effective antimicrobial effect on viable population Thus, Enteritidis ATCC 13076 Schwarzengrund were exposed different time frames, ranging from 5 min 38.5 min, temperatures between °C, NaClO concentrations 0.36 6.36 ppm,...

10.3390/antibiotics13010068 article EN cc-by Antibiotics 2024-01-10

Salmonella spp. is not part of the fish microbiota, being introduced through contaminated water or improper handling. In slaughterhouses, five parts per million (ppm) free chlorine recommended in washing step to eliminate this pathogen. However, still detected processing plants due persistent contamination as a result chlorine–organic matter interactions that reduce effectiveness. Therefore, study aimed test whether organic contained culture media and washes interferes with efficacy at 2 5...

10.3390/microbiolres15010023 article EN cc-by Microbiology Research 2024-02-29

Abstract We investigated bile salts’ ability to induce phenotypic changes in biofilm production and protein expression of pathogenic Escherichia coli strains. For this purpose, 82 E. strains isolated from humans (n = 70), animals 12), were examined for their form biofilms the presence or absence salts. also identified bacterial proteins expressed response salts using sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-electrophoresis) liquid chromatography-mass spectrometry...

10.1093/lambio/ovae032 article EN Letters in Applied Microbiology 2024-04-01

(1) Background: This study aimed to use the simplex-centroid mixture design methodology coupled with a microdilution assay predict optimal essential oil (EO) formulations against three potential foodborne pathogens simultaneously through desirability (D) function. (2) Methods: Oregano (ORE; Origanum vulgare), thyme (THY; Thymus vulgaris), and lemongrass (LG; Cymbopogon citratus) their blends were evaluated concerning minimum inhibitory concentration (MIC) bactericidal (MBC) for Salmonella...

10.3390/antibiotics11111572 article EN cc-by Antibiotics 2022-11-08

We aimed at characterising the nonthermal technologies resistance profile of two Escherichia coli strains isolated from pasteurised milk and described as moderate‐to‐high heat (MHR) (60°C/6 min) harbouring or not a transmissible locus stress tolerance (tLST). The characterisation was carried out applying optimised conditions ultraviolet‐C light‐emitting diodes (UVC‐LED) (4950 mJ/cm 2 ), ultrasound (US) (299 W) their combination (UVC‐LED + US). Both were more sensitive to UVC‐LED (4.5–1.5 log...

10.1111/1471-0307.12925 article EN International Journal of Dairy Technology 2022-12-20

Characterize Escherichia coli and E. -producing (STEC) isolates from Brazilian beef to determine heat resistance the presence of transmissible locus stress tolerance (tLST).Twenty-two STEC previously isolated characterized as by PCR were subjected different survival challenges (60°C 71°C). Furthermore, three tLST-positive one tLST-negative isolate selected for WGS analysis. Phenotypic results indicated that 3/22 (13.64%) resistant, 12/22 (54.54%) moderately 7/22 (31.82%) sensitive...

10.1093/jambio/lxac027 article EN Journal of Applied Microbiology 2022-12-14

Four Escherichia coli isolates with moderate or high heat resistance were sequenced. Through sequencing, truncated transmissible locus of stress tolerance (tLST) variants tLST1 and tLSTa identified in the three isolates. The most tLST genes (clpK hsp) are responsible for homeostasis module.

10.1128/mra.00371-22 article EN Microbiology Resource Announcements 2022-07-21

ABSTRACT: In this study, the kinetic parameters of mesophilic, psychrotrophic and lactic acid bacteria in vacuum-packed beef at 1 °C 4 were estimated from experimental growth curves produced by samples stored during 21 60 days, respectively. a separate experiment, survival multidrug resistant (MDR) Salmonella enterica O:4,5 1°C was also characterized. The shelf-life 16.1 days (95% CI: 14.8 - 17.3 days), whereas it longer than because mesophiles count towards end experiment 12.5 ln CFU.g-1...

10.1590/0103-8478cr20200090 article EN cc-by Ciência Rural 2020-01-01

ABSTRACT: Brazil is the largest exporter of beef world and Mato Grosso State highest producer in this country. To maintain product competitiveness market expansion, sanitary hygienic control entire process indispensable to ensure attainment harmless quality. The objective study was evaluate conditions vacuum-packed produced by establishments qualified for export state Grosso, Brazil. A total 60 samples were submitted coliforms counts at 35°C 45°C E. coli. mean contamination 3,1 x 102MPN/g...

10.1590/0103-8478cr20170526 article EN cc-by-nc Ciência Rural 2018-04-14

Demand for organic food has increased as a result of awareness the health risks posed by presence chemical residues. In this context, study aimed to verify safety lettuce (Lactuca sativa) and arugula (Eruca produced organically in metropolitan region Cuiabá, MT, Brazil, identify main microbiological contamination sources production system. Fifty-five samples, 10 lettuces arugula, were collected farm supermarket, addition five samples each agricultural adjuvant used system (irrigation water,...

10.31413/nativa.v11i1.13592 article EN Nativa 2023-03-30

Ultraviolet-C light-emitting diode (UVC-LED) and ultrasound (US) are two nonthermal technologies with the potential to destroy pathogens. However, little is known about their effectiveness in strains a history of heat resistance. Thus, this study aimed evaluate phenotype genotype heat-resistant extraintestinal pathogenic Escherichia coli (ExPEC) resistance genes after application US, UVC-LED, UVC-LED+US. For this, central composite rotatable designs were used optimize UVC-LED US conditions...

10.1089/fpd.2023.0111 article EN Foodborne Pathogens and Disease 2023-12-13
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