A5076094737- Microtubule and mitosis dynamics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Crystallography and molecular interactions
- Protein Structure and Dynamics
- 14-3-3 protein interactions
- Boron Compounds in Chemistry
- Antibiotic Resistance in Bacteria
- Synthesis and biological activity
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Radioactive element chemistry and processing
- Antimicrobial Resistance in Staphylococcus
- Photosynthetic Processes and Mechanisms
- Carbohydrate Chemistry and Synthesis
- Cancer therapeutics and mechanisms
- Diet, Metabolism, and Disease
- Microbial Metabolites in Food Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- Advanced Fluorescence Microscopy Techniques
- Celiac Disease Research and Management
- Biofuel production and bioconversion
- Erythrocyte Function and Pathophysiology
- Cancer Mechanisms and Therapy
- Nuclear Structure and Function
SASTRA University
2016-2025
Tamil University
2016
Cancer Research UK Scotland Institute
2009-2013
Switch
2013
University of Dundee
2007-2009
Philipps University of Marburg
2009
University of Edinburgh
2008
Heriot-Watt University
2001-2004
Bharathidasan University
2001
Human kinesin Eg5, which plays an essential role in mitosis by establishing the bipolar spindle, has proven to be interesting drug target for development of cancer chemotherapeutics. Here, we report crystal structures Eg5 motor domain complexed with enastron, dimethylenastron, and fluorastrol. By comparing these that monastrol mon-97, identified main reasons increased potency new inhibitors, namely better fit ligand allosteric binding site addition fluorine atoms. We also noticed...
Human kinesin Eg5 is a target for drug development in cancer chemotherapy with compounds phase II clinical trials. These agents bind to well-characterized allosteric pocket involving the loop L5 region, structural element kinesin-5 family members thought provide inhibitor specificity. Using X-ray crystallography, kinetic, and biophysical methods, we have identified characterized distinct able inhibitors nanomolar K(d). This formed by key elements be pivotal force generation kinesins may...
Human kinesin Eg5 plays an essential role in mitosis by separating duplicated centrosomes and establishing the bipolar spindle. is interesting drug target for development of cancer chemotherapy, with seven inhibitors already clinical trials. In present paper, we report crystal structure motor domain complexed a potent antimitotic inhibitor STLC (S-trityl-L-cysteine) to 2.0 A (1 A=0.1 nm) resolution. The Eg5-STLC complex crystallizes space group P3(2) three molecules per asymmetric unit. Two...
The mitotic spindle is a validated target for cancer chemotherapy. Drugs such as taxanes and vinca alkaloids specifically microtubules cause the to collapse. However, toxicity resistance are problems associated with these drugs. Thus, alternative approaches inhibiting being pursued. These include targeting Eg5, human kinesin involved in formation of bipolar spindle. We previously identified S-trityl-l-cysteine (STLC) potent allosteric inhibitor Eg5. Here, we report synthesis new series...
Dihydropyrimidine-based compounds belong to the first discovered inhibitors of human mitotic kinesin Eg5. Although they are used by many research groups as model for chemical genetics, considerably less emphasis has been placed on improvement this type inhibitor, with exception two recent studies. Dihydropyrimidines can be divided into class I (analogues that bind in S configuration) and II inhibitors, which R configuration. Herein we report synthesis optimization novel dihydropyrimidines...
Kinesins constitute a superfamily of microtubule-based motor proteins with important cellular functions ranging from intracellular transport to cell division. Some kinesin family members function during the mitotic phase eukaryotic cycle and are crucial for successful progression In early stages mitosis, prometaphase, certain kinesins required formation bipolar spindle, such as Eg5 Kif15, which seem possess partially overlapping functions. Because transform chemical energy ATP hydrolysis...
New thiazole-thiazolidinedione hybrids (5a-k) were efficiently synthesized and evaluated for their in-vitro antimicrobial activity against four fungal bacterial strains. The chemical structures of the compounds elucidated by FTIR, 1H NMR, 13C NMR spectral data. Most sensitive gram positive, negative Among molecules, 5h, 5i exhibited promising inhibitory all selected strains positive bacteria namely, Staphylococcus aureus, Enterococcus faecalis. molecular docking results predicted that...
Levan is a fructan polymer with many industrial applications such as the formulation of hydrogels, drug delivery, and wound healing, among others. To this end, metabolic systems engineering valuable method to improve yield specific metabolite in wide range bacterial eukaryotic organisms. In study, we report biology approach integrating genomics data for Bacillus subtilis model, wherein pathway levan biosynthesis unpacked. We analyzed revised genome-scale enzyme-constrained model (ecGEM)...
The reactions of the title ylide with HgX2 (X = Cl, Br or I) lead to regiospecific binding acetyl oxygen soft Hg(II), producing a chloro complex (2 + 2) coordination and isostructural dimeric bromo iodo complexes containing halogen bridges tetrahedral configurations around metal centres.
The nonmevalonate route to isoprenoid biosynthesis is essential in Gram-negative bacteria and apicomplexan parasites. enzymes of this pathway are absent from mammals, contributing their appeal as chemotherapeutic targets. One enzyme, 2C-methyl-d-erythritol-2,4-cyclodiphosphate synthase (IspF), has been validated a target by genetic approaches bacteria. Virtual screening against Escherichia coli IspF (EcIspF) was performed combining hierarchical filtering methodology with molecular docking....
Benzochromene (BC) derivatives identified as potent EPI against NorA efflux pump. BC displays 32-fold ciprofloxacin MIC reversal overexpressing mutant. an adjuvant with antibiotic can curtail MDR<italic>S. aureus</italic>.
Vitamin K2, or menaquinone, is an essential cofactor for many organisms and the enzymes involved in its biosynthesis are potential antimicrobial drug targets. One of these enzymes, 1,4-dihydroxy-2-naphthoyl-CoA synthase (MenB) from pathogen Staphylococcus aureus, has been obtained recombinant form quaternary structure analyzed solution. Cubic crystals enzyme allowed a low-resolution (2.9 Å) to be determined. The asymmetric unit consists two subunits crystallographic threefold axis symmetry...
To enhance the antimicrobial and antibiofilm activity of norfloxacin against planktonic biofilm mode growth in ESKAPE pathogens using chemically modified salts.Antimicrobial testing, synergy testing time-kill curve analysis were performed to evaluate antibacterial effect carboxylic acid salts pathogens. In vivo efficacy reduce bacterial bioburden was evaluated zebrafish infection model. Crystal violet assay live-dead staining discern effect. Membrane permeability, integrity molecular docking...
A series of ferulic acid derivatives was synthesized and evaluated for anti-proliferative activity against K562, U937 Hep G2 cells. The compounds were further tested BCR-Abl kinase inhibition.
The title compound, [Cu(C