A5042566637- Nuclear reactor physics and engineering
- Nuclear Materials and Properties
- Nuclear Engineering Thermal-Hydraulics
- Graphite, nuclear technology, radiation studies
- Fusion materials and technologies
- Nuclear and radioactivity studies
- Nuclear Physics and Applications
- Heat transfer and supercritical fluids
- Cancer-related Molecular Pathways
- Chemical Looping and Thermochemical Processes
- Diverse Educational Innovations Studies
- Ubiquitin and proteasome pathways
- Industrial Gas Emission Control
- Heat Transfer and Boiling Studies
- Combustion and Detonation Processes
- Spacecraft and Cryogenic Technologies
- Advanced ceramic materials synthesis
- Microtubule and mitosis dynamics
- Advanced Combustion Engine Technologies
- Catalytic Processes in Materials Science
- Radioactive element chemistry and processing
- Youth Development and Social Support
- Risk and Safety Analysis
- High Temperature Alloys and Creep
- Adsorption and Cooling Systems
Knoxville College
2019-2024
University of Tennessee at Knoxville
2019-2024
Calderdale and Huddersfield NHS Foundation Trust
2024
Oak Ridge National Laboratory
1995-2023
Virginia Tech
1990-2023
Los Alamos National Laboratory
2017-2023
Savannah River National Laboratory
2023
Naval Postgraduate School
2023
Circle Park
2020-2021
Pennsylvania State University
2005-2020
Abstract CDK1 is the only essential cell cycle CDK in human cells and required for successful completion of M-phase. It founding member family conserved across all eukaryotes. Here we report crystal structures complexes CDK1–Cks1 CDK1–cyclin B–Cks2. These confirm nature inactive monomeric fold its ability to be remodelled by cyclin binding. Relative CDK2–cyclin A, B less thermally stable, has a smaller interfacial surface, more susceptible activation segment dephosphorylation shows...
We have prepared phosphorylated cyclin-dependent protein kinase 2 (CDK2) for crystallization using the CDK-activating 1 (CAK1) fromSaccharomyces cerevisiae and grown crystals microseeding techniques. Phosphorylation of monomeric human CDK2 by CAK1 is more efficient than phosphorylation binary CDK2-cyclin A complex. Phosphorylated exhibits histone H1 activity corresponding to approximately 0.3% that observed with fully activated Fluorescence measurements shown Thr160 increases affinity both...
Heterochromatin represents a cytologically visible state of heritable gene repression. In the yeast, Schizosaccharomyces pombe, swi6 encodes heterochromatin protein 1 (HP1)-like chromodomain that localizes to domains, including centromeres, telomeres, and donor mating-type loci, is involved in silencing at these loci. We identify here functional domains swi6p demonstrate from mammalian HP1-like protein, M31, can functionally replace swi6p, showing function conserved yeasts humans....