A5079089142- Multiple Myeloma Research and Treatments
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- Cancer Treatment and Pharmacology
- Liver physiology and pathology
- Nanoparticle-Based Drug Delivery
- Spine and Intervertebral Disc Pathology
- Immune Response and Inflammation
- Bone fractures and treatments
- Orthopedic Infections and Treatments
- Peripheral Neuropathies and Disorders
- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- Bone Metabolism and Diseases
- Lymphatic System and Diseases
- Wound Healing and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- 3D Printing in Biomedical Research
National Institute for Public Health and the Environment
2022-2023
University Hospital Heidelberg
2019
University Medical Center Utrecht
2016-2019
Heidelberg University
2019
University of Twente
2016
Bone marrow niches support multiple myeloma, providing signals and cell-cell interactions essential for disease progression. A 3D bone niche model was developed, in which supportive multipotent mesenchymal stromal cells their osteogenic derivatives were co-cultured with endothelial progenitor cells. These formed networks within the culture, facilitating survival proliferation of primary CD138
Abstract Niches in the bone marrow regulate hematopoietic stem and progenitor cell (HSPC) fate behavior through cell–cell interactions soluble factor secretion. The niche‐HSPC crosstalk is a very complex process not completely elucidated yet. To aid further investigation of this crosstalk, functional vitro 3D model that closely represents main supportive compartments developed. Different combinations human stromal cells hydrogels are tested for their potential to maintain CD34 + HSPCs. Cell...
The bone marrow microenvironment is the preferred location of multiple myeloma, supporting tumor growth and development. It composed a collection interacting subniches, including endosteal perivascular niche. Current in vitro models mimic either these subniches. By developing model combining both niches, this study aims to further enhance ability culture primary myeloma cells vitro. Also, dependency on each niche was studied. A 3D containing two subniches created using bioprinting...
Immune cells and their soluble factors regulate skeletal during normal bone regeneration pathological formation.Bacterial infections can trigger immune responses that activate pro-osteogenic pathways, but these are usually overshadowed by osteolysis concerns of systemic inflammation.The aim this study was to determine whether the transient local inflammatory reaction non-viable bacterial agonists could lead favourable new formation.In a series rabbit studies, as proof-of-concept, it...
To investigate B-cell differentiation and maturation occurring in the germinal center (GC) using vitro culture systems, key factors interactions of GC reaction need to be accurately simulated. This study aims at improving simulation 3D techniques. Human B-cells were incorporated into PEG-4MAL hydrogels, create a synthetic extracellular matrix, supported by CD40L cells, human tonsil-derived lymphoid stromal cytokines. The antibody production CD19+B-cells was best 5.0%-PEG-4MAL, 2.0...
Liposomal drug delivery can improve the therapeutic index of treatments for multiple myeloma. However, an appropriate 3D model in vitro evaluation liposomal is lacking. In this study, we applied a previously developed bone marrow (BM) myeloma to examine therapy.Liposomes different sizes (~75-200 nm) were tested BM model, based on multipotent mesenchymal stromal cells, endothelial progenitor and cells cocultured hydrogel. The behavior efficacy therapy was investigated, evaluating feasibility...
The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long fractures of the lower limbs cannot be restored without intervention and often lead nonunion. Therefore, aim present study was assess pool biological functions mesenchymal stromal cells (hMSCs) isolated from different marrow locations identify novel strategies prime prior their use in fracture healing. Following, ilium, proximal femur, distal femur tibia aspirated hMSCs...