A5026180608- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Marine Biology and Environmental Chemistry
- Marine Sponges and Natural Products
- Periodontal Regeneration and Treatments
- Pluripotent Stem Cells Research
- Multiple Myeloma Research and Treatments
- Orthodontics and Dentofacial Orthopedics
- Cancer Treatment and Pharmacology
- Neuroscience and Neural Engineering
- Cellular Mechanics and Interactions
- Magnesium Alloys: Properties and Applications
- Aluminum Alloys Composites Properties
- Silk-based biomaterials and applications
- Renal and related cancers
- Dental Implant Techniques and Outcomes
- Hydrogels: synthesis, properties, applications
- Electrospun Nanofibers in Biomedical Applications
- Cell Image Analysis Techniques
- Osteoarthritis Treatment and Mechanisms
- Hydrogen Storage and Materials
- Wound Healing and Treatments
- Spine and Intervertebral Disc Pathology
Technische Universität Dresden
2015-2025
University Hospital Carl Gustav Carus
2015-2022
Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs. However, it is still challenging print structures with both, high shape fidelity and cell vitality. Herein, we used synthetic nanosilicate clay, called Laponite, build up scaffolds utilising extrusion-based method 3D plotting. By blending alginate methylcellulose, bioink was developed which allowed easy extrusion, achieving fidelity....
Abstract For the generation of multi-layered full thickness osteochondral tissue substitutes with an individual geometry based on clinical imaging data, combined extrusion-based 3D printing (3D plotting) a bioink laden primary chondrocytes and mineralized biomaterial phase was introduced. A pasty calcium phosphate cement (CPC) alginate-methylcellulose (algMC) – both are biocompatible allow plotting high shape fidelity were applied in monophasic combinatory design to recreate layers. The...
Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest the last couple years as it allows for fabricating constructs with novel and interesting properties. We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels shell material low concentrated, soft biopolymer core leads to mechanically stable robust 3D scaffolds. Alginate, chitosan, gellan gum, gelatin collagen were utilized successfully materials-hydrogels...
Extrusion-based bioprinting, also known as 3D bioplotting, is a powerful tool for the fabrication of tissue equivalents with spatially defined cell distribution. Even though considerable progress has been made in recent years, there still lack bioinks which enable tissue-like response and are plottable at same time good shape fidelity. Herein, we report on development bioink includes fresh frozen plasma from full human blood thus donor/patient-specific protein mixture. By blending 3 w/v%...
Developed concentrated gelatin/alginate with/without HAP composites were plotted into pre-designed scaffolds, which showed good cells attachments and penetration.
Abstract Research on 3D bioprinting of living cells has strong focus printable biocompatible materials and monitoring cell growth in printed constructs, while metabolism is mostly measured media surrounding the constructs or by destructive sample analyses. Bioprinting combined with online imaging O 2 functionalizing a hydrogel bioink via addition luminescent optical sensor nanoparticles. Rheological properties enable printing layers uniform response to concentration. Co‐immobilization...
In tissue engineering, additive manufacturing (AM) technologies have brought considerable progress as they allow the fabrication of three-dimensional (3D) structures with defined architecture. 3D plotting is a versatile, extrusion-based AM technology suitable for processing wide range biomaterials including hydrogels. this study, composites highly concentrated alginate and gellan gum were prepared in order to combine excellent printing properties favorable gelling characteristics gum....
One of the key challenges in biofabrication applications is to obtain bioinks that provide a balance between printability, shape fidelity, cell viability, and tissue maturation. Decellularization methods allow extraction natural extracellular matrix, preserving tissue-specific matrix proteins. However, critical challenge bone decellularization preserve both organic (collagen, proteoglycans) inorganic components (hydroxyapatite) maintain composition functionality bone. Besides, there need...
Abstract Bioprinting enables the integration of biological components into scaffolds during fabrication that has advantage high loading efficiency and better control release and/or spatial positioning. In this study, a biphasic scaffold fabricated by extrusion‐based 3D multichannel plotting calcium phosphate cement (CPC) paste an alginate/gellan gum (AlgGG) hydrogel laden with angiogenic factor VEGF (vascular endothelial growth factor) is investigated regard to response in vitro vivo. Rat...
Scaffolds for bone tissue engineering are essentially characterized by porous three-dimensional structures with interconnected pores to facilitate the exchange of nutrients and removal waste products from cells, thereby promoting cell proliferation in such engineered scaffolds. Although hydroxyapatite is widely being considered applications due its occurrence natural extracellular matrix this tissue, limited reports available on additive manufacturing hydroxyapatite-based materials. In...
The concept of biphasic or multi-layered compound scaffolds has been explored within numerous studies in the context cartilage and osteochondral regeneration. To date, no system identified that stands out terms superior chondrogenesis, osteogenesis formation a zone calcified (ZCC). Herein we present 3D plotted scaffold, comprising an alginate hydroxyapatite paste, cast photocrosslinkable hydrogel made gelatin methacrylamide (GelMA), GelMA with hyaluronic acid methacrylate (HAMA). We...
The bone marrow microenvironment is the preferred location of multiple myeloma, supporting tumor growth and development. It composed a collection interacting subniches, including endosteal perivascular niche. Current in vitro models mimic either these subniches. By developing model combining both niches, this study aims to further enhance ability culture primary myeloma cells vitro. Also, dependency on each niche was studied. A 3D containing two subniches created using bioprinting...
Long-term stability of gellan gum (GG) at physiological conditions is expected, as very low concentration divalent ions are required for crosslinking, compared to alginate—which extensively used tissue engineering (TE) applications. Hence, GG proposed an ideal candidate substitute alginate TE. Deacylated (low acyl; LA) forms brittle gels, thus only concentrations were cell encapsulation, whereas acylated (high HA) weak/soft gels. 3D bioprinting using pure LAGG or HAGG not possible owing...
Soft tissue infections in open fractures or burns are major cause for high morbidity trauma patients. Sustained, long-term and localized delivery of antimicrobial agents is needed early eradication these infections. Traditional (topical systemic) antibiotic methods associated with a variety problems, including their unavailability possible low local concentration. Novel approaches via wound coverage/healing scaffolds constantly being developed. Many burst release thus seldom maintain...
Tissue engineering, the application of stem and progenitor cells in combination with an engineered extracellular matrix, is a promising strategy for bone regeneration. However, its success limited by lack vascularization after implantation. The concept situ tissue engineering envisages recruitment necessary regeneration from host environment foregoing ex vivo cell seeding scaffold. In this study, we developed novel scaffold system enhanced attraction, which based on biomimetic mineralized...
Abstract Magnesium (Mg) alloys are becoming increasingly important in the biomedical field as temporary bone implants. However, biodegradation process of Mg is highly complex and recent findings suggest that oxygen (O 2 ) consumption non-negligible. In this study, we give experimental proof O during degradation under physiological conditions. Specifically, study pure Mg, Mg–6 wt%Ag Mg–5 wt%Gd Hanks’ balanced salt solution Dulbecco’s modified Eagle’s medium. We show hydrogen evolution...