A5018908324- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Bone Tissue Engineering Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Agriculture Sustainability and Environmental Impact
- Electrospun Nanofibers in Biomedical Applications
- Organic Food and Agriculture
- Immune cells in cancer
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Graphene and Nanomaterials Applications
- Pluripotent Stem Cells Research
- Chemokine receptors and signaling
- Receptor Mechanisms and Signaling
- Manufacturing Process and Optimization
- Cellular Mechanics and Interactions
- Silk-based biomaterials and applications
- Medical and Biological Ozone Research
- Immune Cell Function and Interaction
- Craniofacial Disorders and Treatments
- Scoliosis diagnosis and treatment
- Medical Imaging Techniques and Applications
University Hospital Carl Gustav Carus
2017-2025
Technische Universität Dresden
2017-2025
Stanford University
2023-2025
GlaxoSmithKline (United States)
2016-2024
FHNW University of Applied Sciences and Arts
2019
Max Bergmann Zentrum für Biomaterialien
2017
Friedrich-Alexander-Universität Erlangen-Nürnberg
2016
Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs. However, it is still challenging print structures with both, high shape fidelity and cell vitality. Herein, we used synthetic nanosilicate clay, called Laponite, build up scaffolds utilising extrusion-based method 3D plotting. By blending alginate methylcellulose, bioink was developed which allowed easy extrusion, achieving fidelity....
Mouse syngeneic tumor models are widely used tools to demonstrate activity of novel anti-cancer immunotherapies. Despite their widespread use, a comprehensive view tumor-immune compositions and relevance human tumors has only begun emerge. We propose each model possesses unique infiltrate profile that can be probed with immunotherapies inform on anti-tumor mechanisms treatment strategies in similar profiles. In support this endeavor, we characterized the microenvironment four commonly they...
Systematic analysis of the extrusion process in 3D bioprinting is mandatory for optimization concerning production speed, shape fidelity construct and cell viability. In this study, we applied numerical analytical modeling to describe fluid flow inside printing head based on a Herschel-Bulkley model. The presented calculation method nicely reproduces results Computational Fluid Dynamics simulation pressure drop over maximal shear parameters at outlet. An approach with dimensionless parameter...
Abstract For the generation of multi-layered full thickness osteochondral tissue substitutes with an individual geometry based on clinical imaging data, combined extrusion-based 3D printing (3D plotting) a bioink laden primary chondrocytes and mineralized biomaterial phase was introduced. A pasty calcium phosphate cement (CPC) alginate-methylcellulose (algMC) – both are biocompatible allow plotting high shape fidelity were applied in monophasic combinatory design to recreate layers. The...
Due to their characteristic resemblance of the mineral component bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on development pasty phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could demonstrated that sensitive substances like growth factors integrated in such printed CPC scaffolds without impairment...
With the aim of understanding and recapitulating cellular interactions hepatocytes in their physiological microenvironment to generate an artificial 3D vitro model, a co-culture system using extrusion bioprinting was developed. A bioink based on alginate methylcellulose (algMC) first shown be suitable for hepatocytes; addition Matrigel algMC enhanced proliferation morphology them monophasic scaffolds. Towards more complex that allows studying interactions, we applied core-shell establish...
Abstract Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to precise spatial control over depositing biomaterial. Despite their widespread utilization numerous advantages, development suitable novel biomaterials for extrusion-based 3D scaffolds that support cell attachment, proliferation, vascularization remains a challenge. Multi-material composite hydrogels present incredible this field. Thus,...
One of the most common hereditary craniofacial anomalies in humans are cleft lip and alveolar bone with or without palate. Current clinical practice, augmentation persisting defect by using autologous grafts, has considerable disadvantages motivating to an intensive search for alternatives. We developed a novel therapy concept based on 3D printing biodegradable calcium phosphate-based materials integration osteogenic cells allowing fabrication patient-specific, tissue-engineered grafts....
Mechanical stimulation of cells embedded in scaffolds is known to increase the cellular performance toward osteogenic or chondrogenic differentiation and tissue development. Three-dimensional bioplotting magnetically deformable enables spatially defined distribution inducible scaffold regions. In this study, a magnetic bioink based on alginate (alg, 3%) methylcellulose (MC, 9%) with incorporated magnetite microparticles (25% w/w) was developed characterized. The size shape particles were...
Abstract Three-dimensional microextrusion bioprinting has attracted great interest for fabrication of hierarchically structured, functional tissue substitutes with spatially defined cell distribution. Despite considerable progress, several significant limitations remain such as a lack suitable bioinks which combine favorable response high shape fidelity. Therefore, in this work novel bioink alginate-methylcellulose (AlgMC) blend functionalized egg white (EW) was developed the aim solving...
Microextrusion-based 3D bioprinting into support baths has emerged as a promising technique to pattern soft biomaterials complex, macroscopic structures. It is hypothesized that interactions between inks and baths, which are often composed of granular microgels, can be modulated control the microscopic structure within these macroscopic-printed constructs. Using printed collagen bioinks crosslinked either through physical self-assembly or bioorthogonal covalent chemistry, it demonstrated...
One of the key challenges in osteochondral tissue engineering is to define specified zones with varying material properties, cell types and biochemical factors supporting locally adjusted differentiation into osteogenic chondrogenic lineage, respectively. Herein, extrusion-based core-shell bioprinting introduced as a potent tool allowing spatially defined delivery TGF-β3 BMP-2 separated compartments hydrogel strands, and, therefore, local supply matching for chondrocytes osteoblasts. Ink...
In this report the in vitro and vivo pharmacological pharmacokinetic profile of (-)-(S)-N-(alpha-ethylbenzyl)-3-(carboxymethoxy)-2-phenylquinoline-4-carboxamide (SB 235375), a low central nervous system (CNS)-penetrant, human neurokinin-3 (NK-3) receptor (hNK-3R) antagonist, is described. SB 235375 inhibited (125)I-[MePhe(7)]-neurokinin B (NKB) binding to membranes Chinese hamster ovary (CHO) cells expressing hNK-3R (CHO-hNK-3R) with K(i) = 2.2 nM antagonized competitively NKB-induced Ca(2+)...
CXC chemokine receptor 2 (CXCR2) is a key in the chemotaxis of neutrophils to sites inflammation. The studies reported here describe pharmacological characterization danirixin, CXCR2 antagonist diaryl urea chemical class. Danirixin has high affinity for CXCR2, with negative log 50% inhibitory concentration (pIC<sub>50</sub>) 7.9 binding Chinese hamster ovary cell (CHO)-expressed human and 78-fold selectivity over CHO-expressed CXCR1. competitive against CXCL8 Ca<sup>2+</sup>-mobilization...
Abstract Magnetic resonance imaging (MRI) is a common clinical practice to visualize defects and distinguish different tissue types pathologies in the human body. So far, MRI data have not been used model generate patient-specific design of multilayered substitutes case interfacial defects. For orthopedic cases that require highly individual surgical treatment, implant fabrication by additive manufacturing holds great potential. Extrusion-based techniques like 3D plotting allow spatially...
While the human body has many different examples of perfusable structures with complex geometries, biofabrication methods to replicate this complexity are still lacking. Specifically, fabrication self-supporting, branched networks multiple channel diameters is particularly challenging. Here, we present Gelation Uniform Interfacial Diffusant in Embedded 3D Printing (GUIDE-3DP) approach for constructing interconnected channels precise control over branching geometries and vessel sizes. To...
The fabrication of patient-specific scaffolds for bone substitutes is possible through extrusion-based 3D printing calcium phosphate cements (CPC) which allows the generation structures with a high degree customization and interconnected porosity. Given brittleness this clinically approved material, stability open-porous cannot always be secured. Herein, multi-technological approach allowed simultaneous combination CPC melt electrowriting (MEW) polycaprolactone (PCL) microfibers in an...
Abstract Innovative biomaterial‐based concepts are required to improve wound healing of damaged vascularized tissues especially in elderly multimorbid patients. To develop functional hydrogels as 3D cellular microenvironments and carrier or scavenging systems, e.g., for mediator proteins proinflammatory factors, collagen fibrils embedded into a network photo‐crosslinked acrylated hyaluronan (HA), chondroitin sulfate (CS), sulfated HA (sHA). After lyophilization, the gels show porous...
Abstract Periodontitis is a chronic inflammatory and tissue‐destructive disease. Since the polymicrobiome in oral cavity makes it difficult to treat, novel therapeutic strategies are required. Hydrogels based on self‐assembling peptides (SAP) can be suitable candidates for periodontal therapy due their injectability, biocompatibility, cargo‐loading capacity, tunable physicochemical mechanical properties. In this study, two SAP hydrogels (P11‐4 P11‐28/29) examined intrinsic antimicrobial...
Abstract Advances in biofabrication have enabled the generation of freeform perfusable networks mimicking vasculature. However, key challenges remain effective endothelialization these complex, vascular-like networks, including cell uniformity, seeding efficiency, and ability to pattern multiple types. To overcome challenges, we present an integrated fabrication strategy directly generate branched, endothelial cell-lined using a diffusion-based, embedded 3D bioprinting process. In this...
3D extrusion bioprinting, a promising and widely adopted technology in the emerging field of biofabrication, has gained considerable attention for its ability to fabricate hierarchically structured, native-mimicking tissue substitutes with precisely defined cell distributions. Despite notable advancements, limited availability suitably bioactive bioinks remains major challenge, hindering construction volumetric effectively mimicking biological functionality. Therefore, this work proposes...