A5028747142- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Bone fractures and treatments
- Proteoglycans and glycosaminoglycans research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Hemostasis and retained surgical items
- Collagen: Extraction and Characterization
- Cancer Cells and Metastasis
- Endodontics and Root Canal Treatments
- Electrohydrodynamics and Fluid Dynamics
- Pluripotent Stem Cells Research
- Algal biology and biofuel production
- Fluid Dynamics and Heat Transfer
- Radioactive element chemistry and processing
- Planarian Biology and Electrostimulation
- Microfluidic and Bio-sensing Technologies
- Calcium Carbonate Crystallization and Inhibition
- Plant Surface Properties and Treatments
- Osteoarthritis Treatment and Mechanisms
- Diabetes Management and Research
- Mobile Health and mHealth Applications
University Hospital Carl Gustav Carus
2015-2022
Technische Universität Dresden
2014-2022
Institute for Bioprocessing and Analytical Measurement Techniques
2009-2015
Embedding of mammalian cells into hydrogel scaffolds predesigned architecture by rapid prototyping technologies has been intensively investigated with focus on tissue engineering and organ printing. The study demonstrates that such methods can be extended to originating from the plant kingdom. By using 3D plotting, microalgae species Chlamydomonas reinhardtii were embedded in alginate‐based scaffolds. algae survived plotting process able grow within matrix. Under illumination, cell number...
Abstract Bioprinting enables the integration of biological components into scaffolds during fabrication that has advantage high loading efficiency and better control release and/or spatial positioning. In this study, a biphasic scaffold fabricated by extrusion‐based 3D multichannel plotting calcium phosphate cement (CPC) paste an alginate/gellan gum (AlgGG) hydrogel laden with angiogenic factor VEGF (vascular endothelial growth factor) is investigated regard to response in vitro vivo. Rat...
Abstract The application of strontium is one option for the clinical treatment osteoporosis—a disease characterized by reduced bone density and quality—in order to reduce risk vertebral nonvertebral fractures. Unlike other drugs used in osteoporosis therapy, shows a dual effect on metabolism attenuating cellular resorption simultaneously enhancing new tissue formation. Current concerns regarding systemic highly dosed ranelate led development strontium‐modified scaffolds based mineralized...
In this study, the effect of heparin-modified collagen type I/hydroxyapatite (HA) nanocomposites on key processes bone regeneration - osteogenesis and angiogenesis was characterised in vitro. Two approaches were applied for heparin modification: it either integrated during material synthesis (in situ) or added to porous scaffolds after their fabrication (post). Cultivation human marrow-derived stromal cells (hBMSC), versus heparin-free scaffolds, revealed a positive modification...
Abstract The development of biomaterials with intrinsic potential to stimulate endogenous tissue regeneration at the site injury is a main demand on future implants in regenerative medicine. For critical‐sized bone defects, an situ engineering concept devised based biomimetic mineralized collagen scaffolds. These scaffolds are functionalized central depot loaded signaling factor cocktail, obtained from secretome hypoxia‐conditioned human mesenchymal stem cells (MSC). Therefore, medium...
To develop cost-effective and efficient bone substitutes for improved regeneration of defects, heparin-modified mineralized collagen scaffolds were functionalized with concentrated, naturally occurring bioactive factor mixtures derived from adipose tissue, platelet-rich plasma conditioned medium a hypoxia-treated human marrow-derived mesenchymal stem cell line. Besides the analysis release kinetics scaffolds, bioactivity released factors was tested regard to chemotaxis angiogenic tube...
Next-generation bone implants will be functionalized with drugs for stimulating growth. Modelling of drug release by such biomaterials and dispersion into can used as predicting tool testing in future. Therefore, the determination experimental parameters to describe simulate is essential. Here, we focus on Sr2+ transport quantification cortical rat bone. dose-dependently stimulates bone-building osteoblasts inhibits bone-resorbing osteoclasts. It should preferentially applied case fracture...
Bone marrow stromal cells (BMSC) are highly attractive for tissue engineering due to their ability differentiate into different cell types, expand extensively in vitro and release paracrine soluble factors with a high regenerative potential. They were observed migrate towards the sites of injury response chemotactic signals vivo. During last years hypoxia has become proven method control proliferation, differentiation multipotency BMSC. Conditioned medium from hypoxia-treated BMSC...
In this study, the bone-regenerative potential of bioactive factors derived from adipose tissue, platelet-rich plasma (PRP) and conditioned medium hypoxia-treated human telomerase immortalized bone-marrow-derived mesenchymal stem cells (hTERT-MSC) was investigated in vitro with aim to develop cost-effective efficient bone substitutes for optimized regeneration defects. Adipose tissue harvested donors undergoing reconstructive surgery, extract (ATE) prepared. Platelet lysates (PL) were...
Tissue engineering, the application of stem and progenitor cells in combination with an engineered extracellular matrix, is a promising strategy for bone regeneration. However, its success limited by lack vascularization after implantation. The concept situ tissue engineering envisages recruitment necessary regeneration from host environment foregoing ex vivo cell seeding scaffold. In this study, we developed novel scaffold system enhanced attraction, which based on biomimetic mineralized...
Abstract Background Due to their multilineage potential and high proliferation rate, mesenchymal stem cells (MSC) indicate a sufficient alternative in regenerative medicine. In comparison the commonly used 2-dimensional culturing method, as spheroids stimulates cell-cell communication mimics vivo milieu more accurately, resulting an enhanced potential. To investigate osteoregenerative of MSC suspensions, cell-loaded fibrin gels were implanted into murine critical-sized femoral bone defects....
Vascularization is essential for the regeneration of bone tissue within composite material. We measured effect regioselectively modified cellulose/hemicellulose as an additive porous scaffolds collagen/hydroxyapatite nanocomposite on tubule formation human vascular endothelial cells. Using a coculture cells and fibroblasts, formed network tubules incubation time 14 to 24 days. A cellulose sulfate with irregular sulfation pattern along polysaccharide backbone (13-TACS-01) led additional...
In article number 1901426 by Anja Lode and co-workers, sustained release of hypoxia-conditioned MSC secretome from a central depot induces directed hBMSC migration angiogenesis in biomimetic mineralized collagen scaffolds. This situ tissue engineering concept is suggested to be cell-free ready-to-use therapeutic solution for accelerated bone defect healing.