A5102962507- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Marine Biology and Environmental Chemistry
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Periodontal Regeneration and Treatments
- Reproductive Biology and Fertility
- Silk-based biomaterials and applications
- Marine Sponges and Natural Products
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Environmental and Sediment Control
- Adipokines, Inflammation, and Metabolic Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Spaceflight effects on biology
- Retinoids in leukemia and cellular processes
- Xenotransplantation and immune response
- Physiological and biochemical adaptations
- Liver Disease Diagnosis and Treatment
- Surgical Sutures and Adhesives
- Hemostasis and retained surgical items
- Exercise and Physiological Responses
- Renal and related cancers
Czech Academy of Sciences, Institute of Experimental Medicine
2024-2025
Czech Academy of Sciences
2024-2025
Czech Academy of Sciences, Institute of Physiology
2022-2025
Institute for Problems of Cryobiology and Cryomedicine
2014-2024
National Academy of Sciences of Ukraine
2013-2024
UNESCO
2022
V. N. Karazin Kharkiv National University
2021
Ukrainian Medical Stomatological Academy
2018
Abstract The widespread use of multipotent mesenchymal stromal cell-derived secretome (MSC-sec) requires optimal preservation methods. Lyophilization offers benefits like concentrating the secretome, reducing storage volume, and making conditions more flexible. This study evaluated influence duration temperature on lyophilized MSC-sec. conditioned medium from Wharton’s jelly MSCs was stored at – 80 °C or with without trehalose. Lyophilized formulations were kept °C, − 20 4 room (RT) for 3 30...
Cytokine licensing with pro-inflammatory molecules, such as tumour necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ), has emerged a promising strategy to enhance the therapeutic potential of multipotent mesenchymal stromal cells (MSCs). While demonstrated benefits for immunomodulation, its effects on other key MSC functions, including differentiation paracrine activity, remain incompletely explored. In this study, we evaluated transcriptomic, metabolomic, functional changes induced...
Abstract New gelatin-based cryostructurates have been elaborated and tested as scaffolds for three-dimensional (3D) cell culturing. Scaffold preparation included dissolution of Type A gelatin in dimethylsulfoxide, freezing such solution, cryoextraction crystalline phase with cold ethanol, cross-linking carbodiimide ethanol medium, treatment the matrix ethanolic solution Tris tanning formaldehyde dissolved ethanol. The use organic media during all stages ensured sterility scaffolds. matrices...
Cryopreservation is the only established method to provide long-term storage and fast availability of cellular product for therapeutic applications. The overwhelming majority cryopreservation media contain toxic concentrations dimethyl sulfoxide (DMSO) limiting possibility direct administration cryopreserved cells patients. Here, we propose a novel approach nontoxic xeno-free human multipotent mesenchymal stromal (MSCs) aimed at ensuring high viability, ready-to-use availability, localized...
Bone tissue engineering strategy involves the 3D scaffolds and appropriate cell types promoting replacement of damaged area. In this work, we aimed to develop a fast reliable clinically relevant protocol for viable bone grafts, using cryopreserved adipose tissue-derived mesenchymal stromal cells (MSCs) composite collagen-nano-hydroxyapatite (nanoHA) scaffolds. Xeno- DMSO-free MSCs were perfusion-seeded into biomimetic collagen/nanoHA manufactured by cryotropic gelation their...
This paper presents our findings on using skeletons of marine sponge Ianthella basta as the carriers for human mesenchymal stromal cells (MSC), evaluating their biocompatibility with cells, well assessment cryosensitivity growing within these to cryopreservation under protection 10% DMSO and 20% fetal bovine serum according method developed MSC suspension (slow cooling 1 deg/min rate, rapid thawing at 37°С). Network-like chitin consisting fibrils were derived from by acid-base hydrolysis....
Organoids represent indispensable opportunities for biomedicine, including drug discovery, cancer biology, regenerative and personalised medicine or tissue organ transplantation. However, the lack of optimized preservation strategies limits wide use organoids in research clinical fields. In this review, we present a short outline recent developments organoid current cryopreservation systems. While both vitrification slow controlled freezing have been utilized structures their precursor...
Prospects for the widespread use of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine determine relevance studying their abilities to affect reparative process experimental systems vivo.Materials and methods. The effect human adipose-derived MSCs on healing rate completeness damaged skin site reconstitution was examined using full-thickness excision wound model mice. activity revealed planimetric histological studies. Human blood plasma-derived fibrin gel used as a...
Aim . To study the therapeutic potential of cryopreserved fetal liver cells seeded into macroporous alginategelatin scaffolds after implantation to omentum rats with hepatic failure. Materials and methods. Hepatic failure was simulated by administration 2-acetyl aminofl uorene followed partial hepatectomy. Macroporous alginate-gelatin scaffolds, allogenic (FLCs) were implanted rat omentum. prevent from colonization host coated alginate gel shell. Serum transaminase activity, levels albumin...
Keywords: chitosan scaffolds, adipose derived mesenchymal stromal cells, tissue engineering, degree of deacetylation
The aim of the research was to compare shape, viability, metabolic and proliferative activity mesenchymal stromal cells (MSCs) during cultivation in hydrogels macroporous scaffolds. Materials methods. Human adipose tissue MSCs were isolated from lipoaspirates healthy adult donors after obtaining informed consent. Hydrogels obtained platelet-poor human blood plasma alginate polymer, cross-linked with calcium ions microspheres. Macroporous scaffolds prepared by cryotropic gelation method....