The aim was to investigate a possibility of using the cryopreserved human culture fibroblasts (CrHFC) with gold nanoparticles (AuNPs) treat experimental burns in rats.The third-degree were modeled white male rats. All animals divided into three groups: control group no wound treatment; 1 composed CrHFC application; and 2 consisted those AuNPs (6 μg/ml) application burn surface next day after injury. applied methylcellulose gel dose 5 × 10(4) viable cells per cm(2) burn. removed from...

Chickpea ( Cicer arietinum L.) is a key legume crop with significant economic and nutritional importance, often cultivated in drought- prone regions. The development of drought-tolerant varieties crucial for maintaining food security amidst the challenges posed by climate change. This study focuses on differential expression two responsive genes: abscisic acid receptor PYL1 glycine-rich RNA- binding acid-inducible protein-like chickpea genotypes, Desi PI598080 (non-drought-tolerant) Kabuli...

Follicular fluid extracellular vesicles (FF EVs) facilitate communication between oocytes and somatic cells within the ovarian follicle, playing a pivotal role in follicular development. This study highlights molecular functional distinctions small (SEV) large (LEV) FF EV subpopulations, revealing their specialized regulatory roles granulosa cell (GC) biology consequential impact on function. Single-EV profiling uncovered distinct tetraspanin distributions, with LEVs containing lower...

Cell culture models are excellent tools for potential toxicity of nanoparticles and fundamental investigations in cancer research. Thus, information about AuNP effects on human health is necessary the use nanomaterials clinical settings. The aim our research to examine AuNPs epithelial origin cell lines: continuous oncogenic. Embryonic porcine kidney inoculated (SPEV) line colorectal carcinoma (HT29) were used. In test cultures, proliferation, necrosis/apoptosis, multicellular spheroids...

The aim of the present study is to determine what effect different concentrations 15 nm gold nanoparticles (AuNPs) will have on immunophenotype, synthesis collagen type I, ability direct differentiation and spectroscopic characteristics bone marrow mesenchymal stem cells (MSCs). AuNPs in 1.5–9 μ g/ml did not lead changes level expression CD 45, 90, 73. It should be noted that 6 9 led a decrease 44 by 6% 9%, respectively. content 105 was reduced 5% when were applied at concentration g/ml....

Inflammatory disorders account for a significant percentage of gynecologic diseases, particularly in women reproductive age. It is known that stem cells have anti-inflammatory and regenerative properties. Based on this, we investigated the effect intravenous administration cryopreserved mesenchymal (cMSCs) bone marrow experimental chronic inflammation ovaries. The paper shows 21st day after cMSC therapy, leukocyte infiltration ovaries was slightly relative to control group without treatment,...

One of the current trends in biomedical research is use novel biological products and cryotechniques to maintain fertility, especially case planned cytotoxic therapy. Existing protocols testicular tissue cryopreservation require further optimization improvement. This study represents a comparative evaluation effect biopolymers (bovine serum albumin, collagen fibrin gel) combination with either 0.6M dimethylsulfoxide or 0.7 M glycerol on morphofunctional characteristics seminiferous tubules...

Background: Degenerative diseases are a medical, social, and economic problem worldwide. The most significant factors predisposing the development of degenerative changes in intervertebral discs low density poor biosynthetic potential cells. Therefore, stem cell therapy this case should show high clinical efficiency. Methods: research aim was to evaluate regenerative cryopreserved mesenchymal cells (MSCs) upon discs. Rats with simulated damage disc Co6–Co7 were administrated 0.5 × 106 either...

Розробка нових методологічних підходів, спрямованих на збереження потенційної фертильності під час планування цитотоксичної терапії у осіб допубертатного віку, є актуальним напрямком медико-біологічних досліджень. У роботі проведено порівняльну оцінку впливу експозиції середовищах різного композиційного складу морфологічні параметри та метаболічну активність клітин звитих канальців сім'яників статевонезрілих щурів. Кріоконсервування тканин яєчка вимагає застосування відповідних протоколів,...

Today the cause of reproductive disorders in women frequently is inflammation pelvic organs. Chronic ovaries (oophoritis) a common disease, accompanied by various gynecological dis-orders, and occurs independently as well. According to Ministry Healthcare Ukraine, frequency inflammatory diseases organs structure pathology practice reaches 60–65% [1]. As consequence, prolonged recurrent course disease 60% cases can lead infertility. It should be noted that oophoritis largely due presence...

Keywords: Immature testicular tissue, spermatogenic epitelium, cryoprotective medium, collagen gel, fibrin metabolic activity

Autologous transplantation of cryopreserved fragments an immature testis is actively developing approach to save fertility in patients facing a gonadotoxic therapy. The use bioavailable fullerene C60 as powerful antioxidant opens up new potential for the prevention and correction ischemic-reperfusion pathological processes tissues including those associated with freezing-thawing procedure. In this work, we aimed study status, apoptotic/necrotic processes, morphological characteristics...

Prospects for the widespread use of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine determine relevance studying their abilities to affect reparative process experimental systems vivo.Materials and methods. The effect human adipose-derived MSCs on healing rate completeness damaged skin site reconstitution was examined using full-thickness excision wound model mice. activity revealed planimetric histological studies. Human blood plasma-derived fibrin gel used as a...

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The relevance of the chosen topic article is determined by fact that in contemporary digital economy, society growing increasingly reliant on information technologies, efficient data management and necessity to transform knowledge management. aim study substantiate importance transforming make it responsive volatile highlight as a factor development systems elaborate technology capacity management, analyze models applicable economy. analyzes preconditions an effective model for quality...