A5073719192- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Cystic Fibrosis Research Advances
- Glycosylation and Glycoproteins Research
- Nosocomial Infections in ICU
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
University of Minnesota
2019-2024
University Gastroenterology
2022
University of Minnesota Medical Center
2021
Biotechnology Institute
2020
Minot State University
2018
Although both infections and vaccines induce memory B cell (MBC) populations that participate in secondary immune responses, the MBCs generated each case can differ. Here, we compare SARS-CoV-2 spike receptor binding domain (S1-RBD)-specific primary form response to infection or a single mRNA vaccination. Both MBC have similar frequencies blood respond second S1-RBD exposure by rapidly producing plasmablasts with an abundant immunoglobulin (Ig)A+ subset are mostly IgG+ cross-react B.1.351...
In the cystic fibrosis (CF) airways, Pseudomonas aeruginosa undergoes diverse physiological changes in response to inflammation, antibiotic pressure, oxidative stress and a dynamic bioavailable nutrient pool. These include loss-of-function mutations that result reduced virulence, altered metabolism other phenotypes are thought confer selective advantage for long-term persistence. Recently, clinical isolates of P. hyperproduce agmatine (decarboxylated arginine) were cultured from individuals...
Clostridium difficile causes nearly 500,000 infections and 30,000 deaths each year in the U.S., which is estimated to cost $4.8 billion. C. infection (CDI) arises from bacteria colonizing large intestine releasing two toxins, toxin A (TcdA) B (TcdB). Generating humoral immunity against difficile’s toxins provides protection primary recurrence. Thus, a vaccine may offer best opportunity for sustained, long-term protection. We developed novel single-cycle adenovirus (SC-Ad) expressing...
Stable isotope probing (SIP) has emerged as a powerful tool to address key questions about microbiota structure and function. To date, diverse isotopically labeled substrates have been used characterize in situ growth activity of specific bacterial taxa revealed the flux bioavailable through microbial communities associated with health disease. A major limitation field is dearth biologically relevant "heavy" substrates. Mucin glycoproteins, for example, comprise an abundant source carbon...
Immunosuppressed patients with inflammatory bowel disease (IBD) generate lower amounts of SARS-CoV-2 spike antibodies after mRNA vaccination than healthy controls. We assessed S1 receptor binding domain–specific (S1-RBD–specific) B lymphocytes to identify the underlying cellular defects. Patients IBD produced fewer anti–S1-RBD antibody–secreting cells controls first and total neutralizing second. S1-RBD–specific memory were generated same degree in control groups numerically stable for 5...
Clostridioides difficile, an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol be practiced any lab setting using portable glove bag. This also optimizes existing cell growth methods presents detailed trouble shooting guide. is modification of those previously reported by...
ABSTRACT In the cystic fibrosis (CF) airways, Pseudomonas aeruginosa undergoes diverse physiological changes in response to inflammation, antibiotic pressure, oxidative stress and a dynamic bioavailable nutrient pool. These include loss-of-function mutations that result reduced virulence, altered metabolism other phenotypes are thought confer selective advantage for long-term persistence. Recently, clinical isolates of P. hyperproduce agmatine (decarboxylated arginine) were cultured from...