A5081204161- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Carbohydrate Chemistry and Synthesis
- Cancer-related Molecular Pathways
- SARS-CoV-2 and COVID-19 Research
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Proteoglycans and glycosaminoglycans research
- Pancreatic function and diabetes
- Genetics, Bioinformatics, and Biomedical Research
- Influenza Virus Research Studies
- Radiomics and Machine Learning in Medical Imaging
- 3D Printing in Biomedical Research
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Occupational and environmental lung diseases
- Salmonella and Campylobacter epidemiology
University of Georgia
2017-2024
National Institute of Allergy and Infectious Diseases
2023-2024
National Institutes of Health
2023-2024
Analytical Services
2022
National Institute of Immunology
2013-2018
The current emergence of the novel coronavirus pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) demands development new therapeutic strategies to prevent rapid progress mortalities. spike (S) protein, which facilitates viral attachment, entry and membrane fusion is heavily glycosylated plays a critical role in elicitation host immune response. protein comprised two subunits (S1 S2), together possess 22 potential N-glycosylation sites. Herein, we report glycosylation...
Abstract The emergence of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has created need for development new therapeutic strategies. Understanding mode viral attachment, entry and replication become a key aspect such interventions. surface features trimeric spike (S) protein that is essential membrane fusion. S SARS-CoV-2 binds to human angiotensin converting enzyme (hACE2) entry. Herein, we describe glycomic glycoproteomic...
The structural analysis of glycoproteins is a challenging endeavor and under steadily increasing demand, but only very limited number labs have the expertise required to accomplish this task. This tutorial aimed at researchers from fields molecular biology biochemistry that discovered are important in their biological research looking for tools elucidate structure. It provides brief descriptions major most common analytical techniques used glycomics glycoproteomics analysis, including...
Significance Glycosylation is a ubiquitous posttranslational modification of proteins wherein carbohydrates are appended to protein side chains, with myriad functions in molecular and cell biology. The enzymes that break down polysaccharides other recalcitrant polymers nature often decorated two canonical forms glycosylation, N- O-linked glycans, the roles which only partially understood these key enzyme families importance both natural biomass turnover industrial biotechnology. Here, we...
Abstract The current emergence of the novel coronavirus pandemic caused by SARS-CoV-2 demands development new therapeutic strategies to prevent rapid progress mortalities. spike (S) protein, which facilitates viral attachment, entry and membrane fusion is heavily glycosylated plays a critical role in elicitation host immune response. protein comprised two subunits (S1 S2), together possess 22 potential N-glycosylation sites. Herein, we report glycosylation mapping on S1 S2 expressed human...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease 2019 (COVID-19), started in China and quickly spread into a global pandemic. Nucleocapsid protein (N protein) is highly conserved the most abundant coronaviruses thus potential target for both vaccine point-of-care diagnostics. N Protein has been suggested literature as having posttranslational modifications (PTMs), accurately defining these PTMs critical its use medicine. Reports of phosphorylation have...
Abstract Most circulating tumor cells (CTC) are detected as single cells, whereas a small proportion of CTCs in multicellular clusters with stemness properties possess 20- to 100-times higher metastatic propensity than the cells. Here we report that CTC dynamics both singles and response therapies predict overall survival for breast cancer. Chemotherapy-evasive relatively quiescent specific loss ST6GAL1-catalyzed α2,6-sialylation glycoproteins. Dynamic hyposialylation or deficiency ST6GAL1...
Efficient drug delivery at vaginal cavity is often a challenge owing to its peculiar physiological variations including vast differences in pH. Keeping view this attribute of the target site, current work was aimed developing formulation strategies which could overcome and successfully deliver molecules like itraconazole through SLNs. Optimized SLNs with given composition selected for further development into mucoadhesive thermosensitive gel. Stearic acid Compritol 888 (1:1, w/w ratio) as...
Abstract The emergence of the COVID-19 pandemic caused by SARS-CoV-2 has created need for development new therapeutic strategies. Understanding mode viral attachment, entry and replication become a key aspect such interventions. coronavirus surface features trimeric spike (S) protein that is essential membrane fusion. S binds to human angiotensin converting enzyme 2 (hACE2) entry. Herein, we describe glycomic glycoproteomic analysis hACE2 expressed in HEK293 cells. We observed high glycan...
Significance Mesothelin (MSLN) is a cell-surface protein that popular target for antibody-based therapies. We have identified shed MSLN as major obstacle to successful antibody therapies and prepared monoclonal inhibits shedding makes very active CAR T cells whose activity not blocked by merits further preclinical development.
To overcome the challenges in analysis of protein glycosylation, we have developed a comprehensive and universal tool through permethylation glycopeptides their tandem mass spectrometric analysis. This method has potential to simplify glycoprotein by integrating glycan sequencing glycopeptide single experiment. Moreover, glycans with unique glycosidic linkages, particularly from prokaryotes, which are resistant enzymatic or chemical release, could also be detected analyzed this methodology....
Type IV pili can be found on the cell surface of many archaea and bacteria where they play important roles in different processes. The UV-inducible system Sulfolobales (Ups) from crenarchaeal species are essential establishing species-specific mating partners, thereby assisting genome stability. With this work, we show that Sulfolobus have specific regions their Ups subunits, which allow them to interact only with cells same species. Additionally, unique surface-layer N- glycosylation...
Biosynthesis of macromolecules requires precursors such as sugars or amino acids, originating from exogenous/dietary sources, reutilization/salvage degraded molecules, de novo synthesis. Since these sources are assumed to contribute one homogenous pool, their individual contributions often overlooked. Protein glycosylation uses monosaccharides all the above produce nucleotide required assemble hundreds distinct glycans. Here, we demonstrate that cells identify origin/heritage monosaccharide,...
The tumor-associated antigen mesothelin is expressed at high levels on the cell surface of many human cancers, while its expression in normal tissues limited. binding to cancer 125 (CA-125) can lead heterotypic adhesion and tumor metastasis within pleural peritoneal cavities. Immunotherapeutic strategies targeting are being intensively investigated. Here, we report crystal structures that reveal a compact, right-handed solenoid consisting 24 short helices connecting loops. These form...
Permethylation is an essential technique for the detailed structural characterization of glycans by mass spectrometry. However, it requires specialized skills and equipment labor-intensive. While this limits glycan analysis to a handful experts, increasing awareness tremendous importance in physiological processes health disease has drastically raised demand glycans. We have developed simple microplate permethylation method that relies upon solid phase extraction using C18 tips purify...
Several reports have demonstrated that Campylobacter bacteriophage DNA is refractory to manipulation, suggesting these phages encode modified DNA. The characterized jejuni fall into two phylogenetic groups within the Myoviridae: genera Firehammervirus and Fletchervirus Analysis of genomic nucleosides from several by high-pressure liquid chromatography-mass spectrometry confirmed 100% 2'-deoxyguanosine (dG) residues are replaced bases. Fletcherviruses replace dG with 2'-deoxyinosine, while...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease (COVID-19) started at the end of 2019 in Wuhan, China has spread rapidly and became a pandemic. Since there is no therapy available that proven as fully protective against COVID-19, vaccine to protect deadly COVID-19 urgently needed. Nucleocapsid protein (N protein), one most abundant proteins coronaviruses potential target for both development point care diagnostics. The variable mass N (45 60 kDa),...
Sialic acids are abundant in the central nervous system (CNS) and essential for brain development, learning, memory. Dysregulation biosynthesis of sialo-glycoconjugates is known to be associated with neurological disorders, CNS injury, cancer. Metabolic glycan engineering (MGE) bioorthogonal ligation have enabled study biological roles glycans vivo; however, direct investigations sialoglycans been intractable. We report a simple strategy utilizing carbohydrate-neuroactive hybrid (CNH)...
Various biological processes at the cellular level are regulated by glycosylation which is a highly microheterogeneous post-translational modification (PTM) on proteins and lipids. The dynamic nature of can be studied through metabolic incorporation non-natural sugars into glycan epitopes their detection using bio-orthogonal probes. However, this approach possesses significant drawback due to nonspecific background reactions ambiguity sugar metabolism. Here, we report probe-free strategy for...
The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global public health crisis. causative agent, the SARS-CoV-2 virus, enters host cells via molecular interactions between viral spike protein and cell ACE2 surface protein. extensively decorated with up to 66 N-linked glycans. Glycosylation of proteins known function in immune evasion strategies but may also events entry into cells. Here, we show that N-glycosylation at Asn331 Asn343 required for it bind pseudovirus harboring...
Mucin-type O-glycans form one of the most abundant and complex post-translational modifications (PTM) on cell surface proteins that govern adhesion, migration, trafficking hematopoietic cells. Development targeted approaches to probe functions is at an early stage. Among several approaches, small molecules with unique chemical functional groups could modulate glycan biosynthesis a critical tool. Herein, we show metabolism peracetyl N-acyl-d-galactosamine derivatives carrying N-thioglycolyl...
Enhanced N-glycan branching is associated with cancer, but recent investigations supported the involvement of less processed N-glycans. Herein, we investigated how changes in N-glycosylation influence cellular properties neuroblastoma (NB) using rat mutant cell lines, NB_1(-Mgat1), NB_1(-Mgat2) and NB_1(-Mgat3), as well parental line NB_1. The two earlier cells have compromised N-acetylglucosaminyltransferase-I (GnT-I) GnT-II activities. Lectin blotting showed that NB_1(-Mgat3) had decreased...