Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor biosynthesis due to its prevention translational elongation. It broadly used cell biology terms determining the half-life a given and gained much popularity cancer research. Here we present full cycloheximide chase assay our laboratory using lung adenocarcinoma line, CL1-5, as model.

Lung cancer is the most common and deadliest worldwide, approximately 90% of all lung deaths are caused by tumor metastasis. Tumor-derived exosomes could potentially promote metastasis through delivery metastasis-related molecules. However, function underlying mechanism exosomal long noncoding RNA (lncRNA) in remain largely unclear.Cell were purified from conditioned media differential ultracentrifugation observed using transmission electron microscopy, size distributions determined...

Lung cancer has the highest mortality rate in world, and mounting evidence suggests that stem cells (CSCs) are associated with poor prognosis, recurrence, metastasis of lung cancer. It is urgent to identify new biomarkers therapeutic targets for targeting CSCs.We computed single-sample gene set enrichment analysis (ssGSEA) 1554 Reactome sets mRNA expression-based stemness index (mRNAsi)-associated pathways using genome-wide RNA sequencing data 509 patients from The Cancer Genome Atlas (TCGA)...

Abstract Oncolytic viruses (OVs) can selectively infect and lyse cancer cells while simultaneously activating the host immune system making them a prospective class of anticancer immunotherapeutic agents. In particular, mammalian reovirus (MRV) avian (ARV) have gained unique attention for their significant properties clinical potentials. This review explores mechanisms action, immunological interactions, applications in OV therapy. Studies shown that MRV exploits defective antiviral...

Ovarian clear cell carcinoma (OCCC) is an aggressive neoplasm with a high recurrence rate that frequently develops resistance to platinum-based chemotherapy. There are few prognostic biomarkers or targeted therapies exist for patients OCCC. Here, we identified FXYD2, the modulating subunit of Na+/K+-ATPases, was highly and specifically expressed in clinical OCCC tissues. The expression levels FXYD2 were significantly higher advanced-stage positively correlated patients' prognoses. Silencing...

Cancer remains one of the leading causes death worldwide. stem cells (CSCs) are underlying reason for tumor recurrence, progression, and therapeutic resistance. Aptamers synthetic single-stranded oligonucleotides that can specifically bind to various molecular targets. Here, we aim develop an effective aptamer-based biomarker tool targets CSCs cancer therapy. We perform whole-cell-based systematic evolution ligands by exponential enrichment (cell-SELEX) screen DNA aptamers bound lung CSCs,...

In this work we have determined that heat shock protein 90 (Hsp90) is essential for avian reovirus (ARV) replication by chaperoning the ARV p17 protein. modulates formation of Hsp90/Cdc37 complex phosphorylation Cdc37, and chaperone machinery protects from ubiquitin-proteasome degradation. Inhibition inhibitors (17-N-allylamino-17-demethoxygeldanamycin 17-AGG, celastrol) or short hairpin RNAs (shRNAs) significantly reduced expression levels viral proteins virus yield, suggesting functions in...

α-Catulin is an oncoprotein that helps sustain proliferation by preventing cellular senescence. Here, we report α-catulin also drives malignant invasion and metastasis. was upregulated in highly invasive non-small cell lung cancer (NSCLC) lines, where its ectopic expression or short-hairpin RNA-mediated attenuation enhanced limited metastasis, respectively. interacted with integrin-linked kinase (ILK), a serine/threonine protein implicated proliferation, antiapoptosis, invasion,...

Background: The cytoskeletal linker protein α-Catulin has been shown to be important for tumor progression in various cancers. However, its role the regulation of cancer stemness remains unclear. Methods: Phenotypic effects on stem cell (CSC)-like properties and metastasis were examined by vitro sphere formation assay, migration invasion vivo xenografted animal models. Yeast two-hybrid co-immunoprecipitation cycloheximide chase assay performed confirm effect WWP1-mediated degradation KLF5....

The borane-protected dihydridodiphosphinofullerene 1,2,4,15-(PPh2·BH3)2(H)2C60 (1) has been prepared by nucleophilic addition of two [H3B·PPh2]− anions to C60 at low temperature, followed protonation with aqueous HCl. BH3 protection groups 1 can be removed N(C2H4)3N afford 1,2,4,15-(PPh2)2(H)2C60 (2), which reacts Os3(CO)10(NCMe)2 generate the double cluster (Os3(CO)10)2(μ,μ,η3,η3-(PPh2)2(H)2C60) (3). structure 3 determined an X-ray diffraction study, where is linked Os3 clusters through...

The specifics of cell receptor-modulated avian reovirus (ARV) entry remain unknown. By using a viral overlay protein-binding assay (VOPBA) and an in-gel digestion coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), we determined that cell-surface annexin A2 (AnxA2) adhesion G protein-coupled receptor Latrophilin-2 (ADGRL2) modulate ARV entry. Direct interaction between the σC protein AnxA2 ADGRL2 in Vero DF-1 cells was demonstrated situ by proximity ligation assays. short...

Wound healing is one of the major challenges in chronic diseases; current treatment options are less effective with undesirable side effects and expensive. Extensive research carried out to develop cost-effective, natural, biodegradable wound dressings that can reduce oxidative stress inflammation prevent bacterial infections. Curcumin has a plethora therapeutic applications; however, its low solubility limits clinical use.In this study, curcumin nanoparticles (Cur NP) curcumin-chitosan...

In our efforts to understand the nature of metal thiolates, we have explored chemistry cobalt ion supported by (thiolato)phosphine ligand derivatives. Herein, synthesized and characterized a square-planar CoII complex binding with bidentate ligand, Co(PS1″)2 (1) ([PS1″]− = [P(Ph)2(C6H3-3-SiMe3-2-S)]−). The activates O2 form ligand-based oxygenation product, Co(OPS1″)2 (2) ([OPS1″]− [PO(Ph)2(C6H3-3-SiMe3-2-S)]−). addition, an octahedral CoIII tridentate bis(thiolato)phosphine...

Background and objective: Although nodal distant metastasis is rare in T1 lung adenocarcinoma, it related to poor clinical prognosis. Association between galectin-3 (Gal-3) expression level, outcome of adenocarcinoma has not been clarified. Methods: From January 2009 December 2014, 74 patients with surgically resected were enrolled this retrospective cohort study. Patient outcomes followed up until 2019. Gal-3 level primary tumors was assessed immunohistochemically evaluated based on the...

Neuropsychiatric systemic lupus erythematosus (NPSLE) is associated with adverse outcomes; however, imaging abnormalities are only detectable by conventional brain magnetic resonance (MRI) in up to 50% of patients. This study investigated the variability cortical thickness and diffusion tensor (DTI) parameters among patients NPSLE whose morphology appeared normal on MRI.This retrospective enrolled 27 female (median age: 41.0 years, range: 22-63 years) 34 healthy controls 37.0 24-55 years)....

Abstract Background: Ovarian cancer is known the gynecologic malignancy with highest case-to-fatality ratio. Once appear metastasis, 5 years survival rate reduce to 30-40% in these patients. Different hematogenously metastasizing tumors, ovarian cells primarily disseminate within peritoneal cavity. Omentum, a lipid-rich tissue, most common metastatic site of cancer. In addition, adipocyte-released free fatty acid can be used by fuel its growth and proliferation. this study, we attempt...

Figure S2. Chemical structures of the major components identified from aqueous extract Radix Paeoniae Rubra. (PDF 7 kb)

Figure S1. Representative base peak chromatograms of aqueous extract RPR positive (A) and negative (B) ion modes obtained from Bruker HCT Ultra Ion Trap MS spectrometer. Detailed experimental conditions were shown in the text. (PDF 10 kb)