A5040229050- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Developmental Biology and Gene Regulation
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Genetics, Aging, and Longevity in Model Organisms
- Insect and Arachnid Ecology and Behavior
- Physiological and biochemical adaptations
- Neurogenesis and neuroplasticity mechanisms
- Lysosomal Storage Disorders Research
- Ubiquitin and proteasome pathways
- Signaling Pathways in Disease
- Hippo pathway signaling and YAP/TAZ
- Ginger and Zingiberaceae research
- Erythrocyte Function and Pathophysiology
- Alzheimer's disease research and treatments
- Hereditary Neurological Disorders
- Adipose Tissue and Metabolism
- Venomous Animal Envenomation and Studies
- Invertebrate Immune Response Mechanisms
- Sirtuins and Resveratrol in Medicine
University of Chile
2014-2024
Instituto de Neurociencia Biomédica
2004-2024
Neuroscience Institute
2022
Pontificia Universidad Católica de Chile
1990-2021
Howard Hughes Medical Institute
1997-1998
Centro de Estudios Científicos
1993
Recent studies have shown that the epithelial sodium channel (ENaC) is expressed in vascular tissue. However, role ENaC may play responses to vasoconstrictors and NO production has yet be addressed. In this study, contractile of perfused pressurized small-diameter rat mesenteric arteries phenylephrine serotonin were reduced by blockade with amiloride (75.1±3.2% 16.9±2.3% control values, respectively; P <0.01) was dose dependent (EC 50 =88.9±1.6 nmol/L). Incubation benzamil, another...
The acquisition of distinct neuronal fates is fundamental for the function cerebral cortex. We find that development subcerebral projections from layer 5 neurons in mouse neocortex depends on high levels expression transcription factor CTIP1; CTIP1 coexpressed with CTIP2 project to targets and SATB2 those contralateral directly represses Tbr1 5, which appears as a critical step fate. In contrast, lower 6 are required TBR1 expression, directs corticothalamic does not appear play role callosal...
Brain activity is constrained by local availability of chemical energy, which generated through compartmentalized metabolic processes. By analyzing data whole human brain gene expression, we characterize the spatial distribution seven glucose and monocarboxylate membrane transporters that mediate astrocyte–neuron lactate shuttle transfer energy. We found coding for neuronal MCT2 only enriched in cerebral cortex where its abundance inversely correlated with cortical thickness. Coexpression...
The intercellular transport of lactate is crucial for the astrocyte-to-neuron shuttle (ANLS), a model brain energetics according to which neurons are fueled by astrocytic lactate. In this study we show that Drosophila chaski gene encodes monocarboxylate transporter protein (MCT/SLC16A) functions as lactate/pyruvate transporter, demonstrated heterologous expression in mammalian cell culture using genetically encoded FRET nanosensor. prominent central nervous system and it particularly...
Stardust (Sdt) and Discs-Large (Dlg) are membrane-associated guanylate kinases (MAGUKs) involved in the organization of supramolecular protein complexes at distinct epithelial membrane compartments Drosophila. Loss either Sdt or Dlg affects development with severe effects on apico-basal polarity. Moreover, is required for structural functional integrity synaptic junctions. Recent biochemical cell culture studies have revealed that various mammalian MAGUKs can interact mLin-7/Veli/MALS, a...
The synaptic membrane-associated guanylate kinase (MAGUK) scaffolding protein family is thought to play key roles in synapse assembly and plasticity. Evidence supporting these vivo scarce, as a consequence of gene redundancy mammals. genome Drosophila contains only one MAGUK gene, discs large ( dlg ), from which two major proteins originate: DLGA [PSD95 (postsynaptic density 95)-like] DLGS97 [SAP97 (synapse-associated protein)-like]. These differ by the inclusion an L27 domain, important for...
Abstract Lactate/pyruvate transport between glial cells and neurons is thought to play an important role in how brain sustain the high‐energy demand that neuronal activity requires. However, vivo mechanisms characteristics underlie of monocarboxylates are poorly described. Here, we use Drosophila expressing genetically encoded FRET sensors provide ex characterization motor from larval ventral nerve cord. We show lactate/pyruvate coupled protons more efficient than neurons. Glial maintain...
Brains are highly metabolically active organs, consuming 20% of a person’s energy at resting state. A decline in glucose metabolism is common feature across number neurodegenerative diseases. Another the progressive accumulation insoluble protein deposits, it’s unclear if two linked. Glucose brain coupled between neurons and glia, with taken up by glia metabolised to lactate, which then shuttled via transporters neurons, where it converted back pyruvate fed into TCA cycle for ATP production....
The axonal wiring molecule Slit and its Round-About (Robo) receptors are conserved regulators of nerve cord patterning. Robo also contribute to brain circuits. Whether molecular mechanisms regulating these signals modified fit more complex processes is unclear. We investigated the role in Drosophila higher-order circuits identified differences cellular Robo/Slit function. First, we find that signaling by regulated Receptor Protein Tyrosine Phosphatase RPTP69d. RPTP69d increases membrane...
Hereditary spastic paraplegias (HSPs) are characterized by spasticity and weakness of the lower limbs, resulting from length-dependent axonopathy corticospinal tracts. In humans, HSP-related atlastin genes ATL1-ATL3 catalyze homotypic membrane fusion endoplasmic reticulum (ER) tubules. How defects in neuronal Atlastin contribute to axonal degeneration has not been explained satisfactorily. Using Drosophila, we demonstrate that downregulation or overexpression motor neurons results decreased...
Drosophila discs-large (dlg) mutants exhibit multiple developmental abnormalities, including severe defects in neuronal differentiation and synaptic structure function. These have been ascribed to the loss of a single gene product, Dlg-A, scaffold protein thought be expressed many cell types. Here, we describe that additional isoforms arise as consequence different transcription start points alternative splicing dlg. At least five dlg products are predicted. We identified subset dlg-derived...
Structural plasticity of synaptic junctions is a prerequisite to achieve and modulate connectivity within nervous systems, e.g., during learning memory formation. It demands adequate backup systems that allow remodeling while retaining sufficient stability prevent unwanted disintegration. The strength submembranous scaffold complexes, which are fundamental the architecture junctions, likely constitutes crucial determinant stability. Postsynaptic density protein-95 (PSD-95)/ Discs-large...
During axon targeting, a stereotyped pattern of connectivity is achieved by the integration intrinsic genetic programs and response to extrinsic long short-range directional cues. How this coordination occurs subject intense study. Transcription factors play central role due their ability regulate expression multiple genes required sense respond these cues during development. Here we show that transcription factor HNT regulates layer-specific photoreceptor targeting in Drosophila through...
Abstract The most compelling evidence for a functional role of caffeine‐sensitive intracellular Ca 2+ reservoirs in nerve cells derives from experiments on peripheral neurons. However, the properties their ryanodine receptor calcium release channels have not been studied. This work combines single‐cell fura‐2 microfluorometry, [ 3 H]ryanodine binding and recording to examine these stores rat sympathetic neurons superior cervical ganglion. Intracellular measurements showed that possess...
Olfactory receptor neurons (ORNs) respond to odorants with changes in the action potential firing rate. Excitatory responses, consisting of increases, are mediated by a cyclic AMP cascade that leads activation cationic nonselective nucleotide-gated (CNG) channels and Ca2+-dependent Cl- (ClCa) channels. This process takes place olfactory cilia, where all protein components this confined. ORNs from various vertebrate species have also been shown generate inhibitory odor expressed as decreases...