A5102734912- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Genomic variations and chromosomal abnormalities
- Cancer, Hypoxia, and Metabolism
- Folate and B Vitamins Research
- Metabolism and Genetic Disorders
- CAR-T cell therapy research
- Retinal Development and Disorders
- Distributed systems and fault tolerance
- Genomics and Chromatin Dynamics
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Bipolar Disorder and Treatment
- Erythrocyte Function and Pathophysiology
- Retinal Diseases and Treatments
- Advanced Biosensing Techniques and Applications
- Erythropoietin and Anemia Treatment
- Photoreceptor and optogenetics research
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2017
Flexibility is an intrinsic property of proteins and essential for their biological functions. However, because structural flexibility, obtaining high-quality crystals with heterogeneous conformations remain challenging. Here, we show a novel approach to immobilize traditional precipitants onto molecularly imprinted polymers (MIPs) facilitate protein crystallization, especially flexible proteins. By applying this method, the N-terminus human fragile X mental retardation are obtained, whose...
Abstract The tandem Agenet domain (TAD) of fragile X mental retardation protein (FMRP) is considered to be a member the methyl-lysine-binding Tudor “Royal family”. Several groups have reported that TAD binds with methylated histones and plays role in DNA damage responses. FMRP RNA-binding predominantly resident cytoplasm. Therefore, this study, we identified DDX5, FUS, EWSR1 LSM14A as TAD-interacting proteins sensitive F32L and/or Y96L mutation by pull-down assays mass spectrometry. We also...
Surgical resection supplemented with adjuvant chemotherapy is the current preferred treatment for Stage III colorectal cancer (CRC). However, as many 48% of patients who undergo curative eventually suffer from incurable distant recurrence. To investigate molecular mechanisms involved in CRC post-surgical recurrence, we identified a total 146 differentially expressed proteins (DEPs) associated recurrence using TMT-based quantitative mass spectrometry. Among these DEPs, altered expressions...
Abstract Background The congenital stationary night blindness (CSNB) affects the patients' dim light vision or dark adaption by impairing normal function of retina. It is a clinically and genetically heterogeneous disorder can be inherited in an X‐linked, autosomal dominant recessive pattern. Several genetic alterations to genes involved visual signal transduction photoreceptors and/or bipolar cells underlie its pathogenesis. Methods In this study, we used Sanger sequencing next‐generation...
Chemical crosslinking is the most straightforward method to produce bispecific antibodies (BsAb) for arming ex vivo activated cytotoxic T lymphocytes. However, heterogeneous polymers are produced by chemical crosslinking. Currently, it not known under what circumstances or extent further purification needed.In this study, we purified Traut's Reagent-Sulfo-SMCC crosslinked anti-CD3 × anti-HER2 size-exclusion column chromatography and compared capacity of crude forms BsAb in enhancing...