A5040717963- RNA modifications and cancer
- MicroRNA in disease regulation
- Genetic and Kidney Cyst Diseases
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- Hedgehog Signaling Pathway Studies
- Animal Behavior and Reproduction
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Circular RNAs in diseases
- Plant and animal studies
- Cancer Research and Treatments
- Avian ecology and behavior
- Protist diversity and phylogeny
- Diabetes Management and Research
- interferon and immune responses
- Diabetes Treatment and Management
Vanderbilt University Medical Center
2021-2024
Vanderbilt University
2021-2024
St. Elizabeth Healthcare
2021
National Cancer Institute
2017-2019
National Institutes of Health
2019
Center for Cancer Research
2019
SUNY College of Environmental Science and Forestry
2016
State University of New York
2016
Mutations in CEP290, a transition zone protein primary cilia, cause diverse ciliopathies, including Leber congenital amaurosis (LCA) and Joubert-syndrome related disorders (JSRD). We examined cilia biogenesis function cells derived from CEP290-LCA CEP290-JSRD patients. CEP290 was reduced LCA fibroblasts with no detectable impact on cilia; however, optic cups induced pluripotent stem (iPSCs) of patients displayed less developed photoreceptor cilia. Lack JSRD resulted abnormal decreased...
Abstract The intracellular ciliogenesis pathway requires membrane trafficking, fusion, and reorganization. Here, we demonstrate in human cells zebrafish that the F-BAR domain containing proteins PACSIN1 -2 play an essential role ciliogenesis, similar to their binding partner reorganizer EHD1. In mature cilia, PACSINs EHDs are dynamically localized ciliary pocket (CPM) transported away from this structure on tubules along with exit cilium. function early at vesicle (CV) stage promote mother...
Darwin's finches are highly innovative. Recently we recorded for the first time a behavioural innovation in outside foraging context: individuals of four species rubbed leaves endemic tree Psidium galapageium on their feathers. We hypothesised that this behaviour serves to repel ectoparasites and tested repellency P. leaf extracts against parasites negatively affect fitness finches, namely mosquitoes invasive hematophagous fly Philornis downsi. Mosquitoes transmit pathogens which have...
Aim: Extracellular communication via the transfer of vesicles and nanoparticles is now recognized to play an important role in tumor microenvironment interactions. Cancer cells upregulate secrete abundant levels miR-100 miR-125b that can alter gene expression donor recipient cells. In this study, we sought identify targets conclusively demonstrate microRNAs (miRNAs) be functionally transferred from Methods: To , used bioinformatic approaches comparing multiple colorectal cancer (CRC) cell...
Extracellular small RNAs (sRNAs) are abundant in many biofluids, but little is known about their mechanisms of transport and stability RNase-rich environments. We previously reported that high-density lipoproteins (HDLs) mice were enriched with multiple classes sRNAs derived from the endogenous transcriptome, also exogenous organisms. Here, we show human HDL transports tRNA-derived (tDRs) host nonhost species, profiles which found to be altered atherosclerosis. hypothesized binds tDRs...
Epitranscriptomic modifications on RNA play critical roles in stability, processing, and function, partly by influencing interactions with RNA-binding proteins receptors. The role of post-transcriptional cell-free non-coding small (sRNA) remains poorly understood disease contexts. High-density lipoproteins (HDL), which transport sRNAs, can lose their beneficial properties atherosclerosis cardiovascular (ASCVD). We hypothesize that changes to regulatory HDL-sRNAs contribute this dysfunction....
5-fluorouracil (5-FU) has been used for chemotherapy colorectal and other cancers over 50 years. The prevailing view of its mechanism action is inhibition thymidine synthase leading to defects in DNA replication repair. However, 5-FU also incorporated into RNA causing toxicity due metabolism, pseudouridine modification, altered ribosome function. Here, we examine the impact on expression export small RNAs (sRNAs) extracellular vesicles (sEVs). Moreover, assess role regulation...
5-Fluorouracil (5-FU) has been used for chemotherapy colorectal and other cancers over 50 years. The prevailing view of its mechanism action is inhibition thymidine synthase leading to defects in DNA replication repair. However, 5-FU also incorporated into RNA causing metabolism, pseudouridine modification, altered ribosome function. We examined the impact on post-transcriptional small modifications (PTxMs) expression export extracellular vesicles (sEVs). EVs are secreted by all cells...
Abstract Introduction: Extracellular vesicles have been found to be important regulators of intercellular communication between cancer cells. EV cargo varies greatly different cell types, the composition which provides insights into role donor and recipient Cetuximab Resistant Colorectal Cancer (CCCR) cells upregulate miR-100 miR-125b select them as for their respective secreted EVs. These miRNAs are responsible altered gene expression in CCCR can also functionally transferred part EVs...
Atherosclerotic cardiovascular disease (ASCVD) is a complex of uncontrolled hypercholesterolemia and inflammation. High-density lipoproteins (HDL) have been shown to suppress atherogenesis through multiple processes; however, many HDL’s beneficial properties are lost during ASCVD. While the underlying reasons for switch unknown, changes composition chemical HDL cargo likely primary contributing factor. We previously reported that transfer microRNAs recipient cells where they regulate gene...
Abstract Macrophages present a spectrum of phenotypes that mediate both the pathogenesis and resolution atherosclerotic lesions. Inflammatory macrophage are pro-atherogenic, but natural factors instigate this polarization largely unknown. Here, we demonstrate microbial small RNAs (msRNA) enriched on LDL drive pro-inflammatory cytokine secretion via activation ribonucleic acid sensor toll-like receptor 8 (TLR8). Removal msRNA cargo during re-constitution yields particles readily promote...
HDL have many alternative functions outside of cholesterol efflux likely due to its diverse cargo proteins, lipids, and small non-coding RNAs (sRNA). We reported that transports microRNAs (miRNA) other sRNA classes, including tRNA-derived sRNAs (tDR), which are produced during cell stress. hypothesize HDL-tDR transport is conferred by apolipoprotein A-I (apoA-I) RNA binding capacity. HDL-miRNAs previously been shown be altered in diseases; however, changes HDL-tDRs diseases such as...
Low-density lipoproteins (LDL) are a causal factor for atherosclerosis, key pathological process in cardiovascular disease. Within atherosclerotic lesions, macrophages present spectrum of phenotypes that mediate both disease pathogenesis and resolution. Inflammatory macrophage pro-atherogenic, but the natural factors promote polarization remain to be identified. Here, we demonstrate microbial small RNAs (msRNA) enriched on LDL drive pro-inflammatory cytokine secretion via activation...
ABSTRACT Extracellular small RNAs (sRNA) are abundant in many biofluids, but little is known about their mechanisms of transport and stability RNase-rich environments. We previously reported that high-density lipoproteins (HDL) mice were enriched with multiple classes sRNA derived from the endogenous transcriptome, also exogenous organisms. Here, we show human HDL transports tRNA-derived sRNAs (tDRs) host non-host species which found to be altered atherosclerosis. hypothesized binds tDRs...