A5074710761- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Diet and metabolism studies
- Plasmonic and Surface Plasmon Research
- RNA and protein synthesis mechanisms
- Nutrition, Genetics, and Disease
- Orbital Angular Momentum in Optics
- Nutritional Studies and Diet
- Extracellular vesicles in disease
- Cancer, Lipids, and Metabolism
- Immune cells in cancer
- Near-Field Optical Microscopy
Vanderbilt University Medical Center
2022-2024
Vanderbilt University
2023-2024
This study addresses the challenge of trapping nanoscale biological particles using optical tweezers without photothermal heating effect and limitation presented by diffraction limit. Optical are effective for microscopic objects but not specimens due to To overcome this, we present an approach that uses anapole states in all-dielectric nanoantenna systems on distributed Bragg reflector substrates generate strong gradient force potential with negligible temperature rise below 1 K. The...
Extracellular small RNAs (sRNAs) are abundant in many biofluids, but little is known about their mechanisms of transport and stability RNase-rich environments. We previously reported that high-density lipoproteins (HDLs) mice were enriched with multiple classes sRNAs derived from the endogenous transcriptome, also exogenous organisms. Here, we show human HDL transports tRNA-derived (tDRs) host nonhost species, profiles which found to be altered atherosclerosis. hypothesized binds tDRs...
5-fluorouracil (5-FU) has been used for chemotherapy colorectal and other cancers over 50 years. The prevailing view of its mechanism action is inhibition thymidine synthase leading to defects in DNA replication repair. However, 5-FU also incorporated into RNA causing toxicity due metabolism, pseudouridine modification, altered ribosome function. Here, we examine the impact on expression export small RNAs (sRNAs) extracellular vesicles (sEVs). Moreover, assess role regulation...
Background: The Japanese population consumes a diet high in rice. Dietary whole-grain (brown) rice has advantageous health benefits over white rice, largely predicted to be conferred by brown bran, germ, and fiber. We have previously reported that high-density lipoproteins (HDL) transport microRNAs (miRNA) deliver functional RNA recipient hepatocytes. Here, we hypothesize dietary reorganizes small (sRNA) cargo on circulating HDL regulates systemic lipid metabolism through hepatic HDL-miRNA...
5-Fluorouracil (5-FU) has been used for chemotherapy colorectal and other cancers over 50 years. The prevailing view of its mechanism action is inhibition thymidine synthase leading to defects in DNA replication repair. However, 5-FU also incorporated into RNA causing metabolism, pseudouridine modification, altered ribosome function. We examined the impact on post-transcriptional small modifications (PTxMs) expression export extracellular vesicles (sEVs). EVs are secreted by all cells...
Abstract Immune checkpoint blockade has been shown to be an effective treatment for colorectal cancers (CRCs) highly infiltrated by CD8+ T cells. However, there is a paucity of T-cell infiltration in the majority CRCs. We previously identified collagen-activated receptor Discoidin Domain Receptor 1 (DDR1) as member four-gene signature associated with exclusion CRC. The cleaved ectodomain (cECD) DDR1 induces collagen alignment, leading breast cancer model. found that almost all cECD...
Epitranscriptomic modifications on RNA play critical roles in stability, processing, and function, partly by influencing interactions with RNA-binding proteins receptors. The role of post-transcriptional cell-free non-coding small (sRNA) remains poorly understood disease contexts. High-density lipoproteins (HDL), which transport sRNAs, can lose their beneficial properties atherosclerosis cardiovascular (ASCVD). We hypothesize that changes to regulatory HDL-sRNAs contribute this dysfunction....
Oxidized phospholipids (OxPL) are potent proinflammatory mediators that can trigger toll-like receptor 2 (TLR2) signaling and inflammasome activation in macrophages. Plasminogen (PLG), a key enzyme the fibrinolytic pathway, has been shown to harbor OxPL adducts. We hypothesized PLG drive activity macrophages through of TLR2 fibrin-independent mechanism. To assess PLG-induced macrophage activation, total RNA sequencing was used quantify gene changes response human treatments, we observed...