A5057963999- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Cancer Cells and Metastasis
- Protein Tyrosine Phosphatases
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- Higher Education Practises and Engagement
- Cancer Mechanisms and Therapy
- Reflective Practices in Education
- Ferroptosis and cancer prognosis
- Hedgehog Signaling Pathway Studies
- Cancer Genomics and Diagnostics
- Galectins and Cancer Biology
- Cancer, Stress, Anesthesia, and Immune Response
- Cellular transport and secretion
- 14-3-3 protein interactions
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- Cellular Mechanics and Interactions
- Collaborative Teaching and Inclusion
- Cancer Research and Treatments
National Cancer Institute
2014-2023
Frederick National Laboratory for Cancer Research
2010-2023
Aurora College
2023
Center for Cancer Research
2019-2022
Cancer Institute (WIA)
2021
National Institutes of Health
2019
Frederick Community College
2014
Cancer Genetics (United States)
2014
Abstract The intracellular ciliogenesis pathway requires membrane trafficking, fusion, and reorganization. Here, we demonstrate in human cells zebrafish that the F-BAR domain containing proteins PACSIN1 -2 play an essential role ciliogenesis, similar to their binding partner reorganizer EHD1. In mature cilia, PACSINs EHDs are dynamically localized ciliary pocket (CPM) transported away from this structure on tubules along with exit cilium. function early at vesicle (CV) stage promote mother...
The majority of hepatocellular carcinoma develops in the background chronic liver inflammation caused by viral hepatitis and alcoholic or nonalcoholic steatohepatitis. However, impact different types inflammatory microenvironments on phenotypes tumors generated distinct oncogenes is largely unresolved. To address this issue, we murine constitutively active AKT‐1 (AKT) β‐catenin (CAT), followed induction 3,5‐diethoxycarbonyl‐1,4‐dihydrocollidine (DDC) carbon tetrachloride. Also, DDC‐induced...
SUMMARY BRAF is frequently mutated in human cancer and the RASopathy syndromes, with mutations often observed cysteine-rich domain (CRD). Although CRD participates phosphatidylserine (PS) binding, RAS-RAF interaction, RAF autoinhibition, impact of these activities on function normal disease states not well-characterized. Here, we analyze a panel show that they increase activity by relieving autoinhibition and/or enhancing PS relief being major factor determining mutation severity zebrafish...
Abstract Many tumors have been demonstrated to contain cancer stem cells (CSC). We hypothesized that expression of transcription factors are required for induction and maintenance pluripotency would be a possible alternative cell surface phenotype identification CSC populations. Therefore we evaluated Nanog in 4T1.2 murine breast carcinoma cells, as biomarker sub-populations. Introduction promoter reporter drives destabilized GFP into resulted stable about 1-3% the cells. On vitro culture...
Abstract Pancreatic Ductal Adenocarcinoma (PDAC) is among the most malignant cancer, with 5-year survival of a mere 6%. Chronic inflammation associated an increased risk PDAC. Nitric oxide (NO•) important mediator immune and inflammatory responses plays role in tumorigenesis. The principal source sustained level NO• inducible nitric synthase (NOS2). In this study, we have tested hypothesis that enhances tumor progression pancreatic cancer. We found higher NOS2 expression tumors was poor...
Abstract The RAS-MAPK pathway is a hotspot for somatic mutations in human cancer and germline the RASopathy developmental syndromes. Understanding regulatory mechanisms that govern this critical identifying new drug targets establishing effective therapeutic approaches patients alike. RASopathies are caused by many of same genes as those found cancer, however must be tolerated during development result tend to distinct terms their location within protein or which family member isoform they...
Co-creation is an open practice where learners participate in decision-making about aspects of course design, which our context has included various activities from design to assessment decisions. After the OTESSA22 conference, this group conference attendees reflected on co-creation practices and experiences their respective post-secondary contexts. In article we share reflections challenges with as well ideas potentially overcome these challenges. This article, examples shared practice,...
Abstract Current evidence supports the view that cancer stem cells (CSCs) constitute primary pathogenic component of both metastases and minimal residual disease elimination CSCs is required for cure. Cell surface markers have been used to identify but fail CSC in all tumors from same tissue or patients. Therefore there a need define test more reliable CSCs. Oct-4, Sox-2 Nanog transcription factors are key regulatory maintenance pluripotency demonstrated be robust intracellular biomarkers...
Abstract Constitutively active AKT, MET, and beta-catenin (CAT) are initiating oncogenes that efficiently induce hepatic primary tumors when co-delivered; but not as single agents. Using a qPCR assay, we have begun to detail the early events of oncogene integration correlate amount integrated with developing tumor size. Furthermore, found serum from mice injected MET/CAT elevated levels alpha fetoprotein, biomarker which has been similarly observed in advanced cases HCC. Correlating influx...
Abstract Worldwide the incidence of hepatocellular carcinoma (HCC) resulting from hepatitis C infection and chronic inflammation is increasing. Poor prognosis HCC characterized by over-expression Ep-CAM alpha-fetoprotein (AFP) which define a population tumor-initiating cells with stem/progenitor features. Current evidence supports view that cancer stem (CSCs) constitute primary pathogenic component both metastases minimal residual disease, elimination CSCs required for cure. Typically cell...
s: Abstracts for the 20th Annual Scientific Meeting of International Society Biological Therapy Cancer (Primary Authors are Italicized): CYTOKINES: BIOLOGY AND THERAPEUTICS