A5058506309- Endoplasmic Reticulum Stress and Disease
- Enzyme Structure and Function
- S100 Proteins and Annexins
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Phytochemistry and Bioactive Compounds
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- X-ray Diffraction in Crystallography
- HIV Research and Treatment
- Photonic and Optical Devices
- Nuclear Structure and Function
- Plasmonic and Surface Plasmon Research
- Cancer, Hypoxia, and Metabolism
- Crystallization and Solubility Studies
- Bone and Dental Protein Studies
- Microtubule and mitosis dynamics
- Protein Kinase Regulation and GTPase Signaling
- Metamaterials and Metasurfaces Applications
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Advanced Electron Microscopy Techniques and Applications
University of Oxford
2024-2025
McGill University
2019-2024
Centre for Human Genetics
2024
McMaster University
2019
China People's Public Security University
2018
University of St Andrews
2014
University of Notre Dame
2010
Abstract Inositol hexakisphosphate (IP6) promotes HIV-1 assembly via its interaction with the immature Gag lattice, effectively enriching IP6 within virions. During particle maturation, protease cleaves polyproteins comprising releasing from original binding site and liberating capsid (CA) domain of Gag. then mature CA protein into shell viral core, which is required for infection new target cells. Recently, we reported mutants that assemble virions independently IP6. However, these are...
Abstract Lentiviruses like HIV-1 infect non-dividing cells by traversing the nuclear pore, but studying this process has been challenging due to its scarcity and dynamic nature in infected cells. Here, we developed a robust cell-permeabilization system that recapitulates import established an integrated cryo-correlative workflow combining cryo-CLEM, cryo-FIB, cryo-ET for targeted imaging of process. These advancements enabled successful capture 1,899 cores at various stages import....
The β-clamp is a protein hub central to DNA replication and fork management. Proteins interacting with the harbor conserved clamp-binding motif that often found in extended regions. Therefore, clamp interactions have -almost exclusively- been studied using short peptides recapitulating binding motif. This approach has revealed molecular determinants mediate but cannot describe how proteins motifs embedded structured domains are recognized. mismatch repair MutL an internal motif, its...
HIV-1 assembly is initiated by the binding of Gag polyproteins to inner leaflet plasma membrane, mediated myristylated matrix (MA) domain Gag. Subsequent membrane binding, oligomerizes and buds as an immature, non-infectious virus particle, which, upon cleavage precursor viral protease, transforms into a mature, infectious virion. During maturation, MA lattice underlying undergoes structural rearrangement newly released capsid (CA) protein forms mature that encloses genome. While it well...
Abstract Tn7 transposable elements are unique for their highly specific, and sometimes programmable, target-site selection mechanisms precise insertions. All the in Tn7-family utilize a AAA+ adaptor (TnsC) to coordinates with transposase activation prevent insertions at sites already containing element. Due its multiple functions, TnsC is considered linchpin Here we present high-resolution cryo-EM structure of bound DNA using gain-of-function variant protein substrate that together...
Motivation: Integration of MRI and spatial transcriptomics may provide a new approach for imaging-genetics research probing molecular basis underlying observed phenotypes. Goal(s): We proposed pipeline integration diffusion MRI(dMRI) mouse brain to understand potential biological pathway dMRI microstructural models. Approach: spatially co-register population-averaged maps 2D transcriptomic pixelwise correlation explored genes function celltype related with metrics. Results: found FA was...
We present our recent results on the design, fabrication and applications of flexible metamaterials in visible near infrared range. In particular, we demonstrate broad angle spectral filtering for lab-on-fibre applications, realisation with epsilon-near-zero response their dynamical tuning via all-optical methods.