A5031786339- Alzheimer's disease research and treatments
- Autophagy in Disease and Therapy
- Liquid Crystal Research Advancements
- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- Photonic Crystals and Applications
- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- Dental materials and restorations
- Dermatology and Skin Diseases
- Drug Solubulity and Delivery Systems
- Organic Electronics and Photovoltaics
- Conducting polymers and applications
- Plant Reproductive Biology
- Oral and Maxillofacial Pathology
- Endodontics and Root Canal Treatments
- Photonic and Optical Devices
- Allergic Rhinitis and Sensitization
- Advanced Drug Delivery Systems
- Advanced Sensor and Energy Harvesting Materials
- Asthma and respiratory diseases
- Pharmacological Effects of Natural Compounds
- Skin and Cellular Biology Research
- Long-Term Effects of COVID-19
- Crystallization and Solubility Studies
Nathan Kline Institute for Psychiatric Research
2009-2024
Kyung Hee University
2012-2024
NYU Langone Health
2010-2024
New York University
2005-2023
Korea Innotech (South Korea)
2023
Seoul National University Bundang Hospital
2017-2023
Sahmyook University
2018-2022
Wonkwang University
2020-2021
Chung-Ang University
2013-2020
Dunsan Korean Medicine Hospital
2020
Macroautophagy, which is a lysosomal pathway for the turnover of organelles and long-lived proteins, key determinant cell survival longevity. In this study, we show that neuronal macroautophagy induced early in Alzheimer's disease (AD) before β-amyloid (Aβ) deposits extracellularly presenilin (PS) 1/Aβ precursor protein (APP) mouse model β-amyloidosis. Subsequently, autophagosomes late autophagic vacuoles (AVs) accumulate markedly dystrophic dendrites, implying an impaired maturation AVs to...
Opioid receptors regulate neuronal activity by both pre- and postsynaptic mechanisms. We recently reported that the cloned delta-opioid receptor (DOR1) is primarily targeted to axons, suggesting a presynaptic role. In present study we have studied distribution targeting of another opioid receptor, mu-opioid (MOR1), raising anti-peptide antisera C-terminal peptide MOR1. The specificity was determined analysis transfected cells, Western blots, immunoisolation studies. Immunohistochemistry...
Autophagy is markedly impaired in Alzheimer's disease (AD). Here we reveal unique autophagy dysregulation within neurons five AD mouse models vivo and identify its basis using a neuron-specific transgenic mRFP-eGFP-LC3 probe of pH, multiplex confocal imaging correlative light electron microscopy. Autolysosome acidification declines well before extracellular amyloid deposition, associated with lowered vATPase activity build-up Aβ/APP-βCTF selectively enlarged de-acidified autolysosomes. In...
Abstract Autophagy is a lysosomal degradative process which recycles cellular waste and eliminates potentially toxic damaged organelles protein aggregates. The important cytoprotective functions of autophagy are demonstrated by the diverse pathogenic consequences that may stem from dysregulation in growing number neurodegenerative disorders. In many diseases associated with anomalies, it final stage autophagy–lysosomal degradation disrupted. several disorders, including A lzheimer's disease...
Presenilin 1 (PS1) deletion or Alzheimer's disease (AD)-linked mutations disrupt lysosomal acidification and proteolysis, which inhibits autophagy. Here, we establish that this phenotype stems from impaired glycosylation instability of vATPase V0a1 subunit, causing deficient assembly function. We further demonstrate elevated pH in knockout (PS1KO) cells induces abnormal Ca(2+) efflux lysosomes mediated by TRPML1 elevates cytosolic Ca(2+). In WT cells, blocking activity knockdown either PS1...
alpha-Synuclein is a component of the abnormal protein depositions in senile plaques and Lewy bodies Alzheimer's disease (AD) Parkinson's respectively. The was suggested to provide possible nucleation centre for plaque formation AD via selective interaction with amyloid beta/A4 (Abeta). We have shown previously that alpha-synuclein has experienced self-oligomerization when Abeta25-35 present an orientation-specific manner sequence. Here we examine this biochemically specific use various...
This work presents a novel approach for producing gastro-retentive floating tablets (GRFT) by coupling hot-melt extrusion (HME) and fused deposition three-dimensional printing (3DP). Filaments containing theophylline (THEO) within hydroxypropyl cellulose (HPC) matrix were prepared using HME. 3DP with different infill percentages shell thickness developed evaluated to determine their drug content, behavior, dissolution, physicochemical properties. The dissolution studies revealed relationship...
Neuronal endosomal dysfunction, the earliest known pathobiology specific to Alzheimer's disease (AD), is mediated by aberrant activation of Rab5 triggered APP-β secretase cleaved C-terminal fragment (APP-βCTF). To distinguish pathophysiological consequences overactivated itself, we activate independently from APP-βCTF in PA-Rab5 mouse model. We report that overactivation alone recapitulates diverse prodromal and degenerative features AD. Modest neuron-specific transgenic expression inducing...
Dysfunction and mistrafficking of organelles in autophagy- endosomal-lysosomal pathways are implicated neurodegenerative diseases. Here, we reveal selective vulnerability maturing degradative (late endosomes/amphisomes) to disease-relevant local calcium dysregulation. These undergo exclusive retrograde transport axons, with occasional pauses triggered by regulated efflux from agonist-evoked transient receptor potential cation channel mucolipin subfamily member 1 (TRPML1) channels—an effect...
Lysosome dysfunction arises early and propels Alzheimer's disease (AD). Herein, we show that amyloid precursor protein (APP), linked to early-onset AD in Down syndrome (DS), acts directly via its β-C-terminal fragment (βCTF) disrupt lysosomal vacuolar (H+)-adenosine triphosphatase (v-ATPase) acidification. In human DS fibroblasts, the phosphorylated 682YENPTY internalization motif of APP-βCTF binds selectively within a pocket v-ATPase V0a1 subunit cytoplasmic domain competitively inhibits...
Autophagy-lysosome pathway (ALP) disruption is considered pathogenic in multiple neurodegenerative diseases; however, current methods are inadequate to investigate macroautophagy/autophagy flux brain vivo and its therapeutic modulation. Here, we describe a novel autophagy reporter mouse (TRGL6) stably expressing dual-fluorescence-tagged LC3 (tfLC3, mRFP-eGFP-LC3) by transgenesis selectively neurons. The tfLC3 probe distributes widely the central nervous system, including spinal cord....
The purpose of our study was to evaluate the effect photodynamic therapy (PDT), using erythrosine as a photosensitizing agent and dental halogen curing unit light source, on Streptococcus mutans in biofilm phase. S. biofilms were formed 24-well cell culture cluster. Test groups consisted divided into four groups: group 1: no photosensitizer or irradiation treatment (control group); 2: alone; 3: 4: irradiation. After treatments, numbers colony-forming (CFU) counted samples examined by...
In traditional Korean medicines, Magnolia officinalis is commonly included for the remedy of atopic dermatitis, and magnolol a major constituent officinalis. Its pharmacological effects include anti-inflammatory, hepatoprotective, antioxidant effects. Using BALB/c mice repeatedly exposed to 1-chloro-2,4-dinitrobenzene (DNCB), was evaluated in dermatitis-like lesions. Administration (10 mg/kg, intraperitoneal injection) markedly relieved skin lesion severity including cracking, edema,...
Increased production and deposition of the 40-42-amino acid β-amyloid peptide (Aβ) is believed to be central pathogenesis Alzheimer's disease. Aβ derived from amyloid precursor protein (APP), but mechanisms that regulate APP processing produce are not fully understood. X11α (also known as munc-18-interacting protein-1 (Mint1)) a neuronal adaptor binds modulates in transfected non-neuronal cells. To investigate vivo effect on brain, we created transgenic mice overexpress crossed these with...
Ninety-three patients undergoing surgical or endovascular operation secondary to aneurysmal subarachnoid hemorrhage (SAH) were retrospectively analyzed determine the influence of different time points C-reactive protein (CRP) measurement on prediction vasospasm and clinical outcome.Laboratory data such as CRP level white blood cell count, preoperative demographic data, intraoperative postoperative complications intracerebral hemorrhage, hydrocephalus, vasospasm, decompression collected at...
Accumulation of cerebral amyloid β-protein (Aβ) is believed to be part the pathogenic process in Alzheimer's disease. Aβ derived by proteolytic cleavage from a precursor protein, protein (APP). APP type-1 membrane-spanning and its carboxyl-terminal intracellular domain binds X11β, neuronal adaptor protein. X11β has been shown inhibit production transfected non-neuronal cells culture. However, whether this also case vivo brain can deposition as plaques not known. Here we show that transgenic...
A novel transflective liquid crystal display architecture and its system driving schemes are proposed. In the reflective mode, ambient light is used to readout displayed images. While in transmissive a color-sequential emitting diode backlight eliminate color filters. Under such device configuration, several advantages as increased brightness maximized saturation for both modes can be achieved