A5081837183- Retinoids in leukemia and cellular processes
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Bone Tissue Engineering Materials
- Renal and related cancers
- Chromatin Remodeling and Cancer
- Additive Manufacturing and 3D Printing Technologies
- Biometric Identification and Security
- Cancer-related gene regulation
- 3D Printing in Biomedical Research
- Face recognition and analysis
- Acute Lymphoblastic Leukemia research
- Sarcoma Diagnosis and Treatment
- Face and Expression Recognition
- Renal cell carcinoma treatment
- Biotin and Related Studies
- Antioxidant Activity and Oxidative Stress
- Orthopaedic implants and arthroplasty
- Epigenetics and DNA Methylation
- Bone Tumor Diagnosis and Treatments
- Cancer Genomics and Diagnostics
- biodegradable polymer synthesis and properties
- Chronic Myeloid Leukemia Treatments
- Cardiac tumors and thrombi
- Cancer Mechanisms and Therapy
National Center For Child Health and Development
2015-2022
Abstract Translocations of retinoic acid receptor-α (RARA), typically PML–RARA, are a genetic hallmark acute promyelocytic leukemia (APL). However, because small fraction APL lack translocations RARA, we focused here on cases without RARA translocation to elucidate the molecular etiology RARA-negative APL. We performed whole-genome sequencing, PCR, and FISH for five translocations. Four had involving receptor-β (RARB) translocations, TBL1XR1–RARB was identified as an in-frame fusion in three...
Abstract Clear cell sarcoma of the kidney (CCSK) is second most common renal malignancy in children. The prognosis poorer CCSK than Wilms’ tumor, and multimodal treatment including surgery, intensive chemotherapy, radiation required to improve outcome for children with CCSK. Histological evaluation diagnosis. However, biopsies tumors obtain diagnostic specimens are not routinely performed because risk spreading tumor cells during procedure. Recently, internal tandem duplication (ITD) BCOR...
Detection of the tumor-specific EWSR1/FUS-ETS fusion gene is essential to diagnose Ewing sarcoma. Reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization are commonly used detect gene, assays using next-generation sequencing have recently been reported. However, at least 28 transcript variants reported, making rapid accurate detection difficult.We constructed two sets multiplex PCR evaluated their utility cell lines clinical samples.EWSR1/FUS-ETS was...
Deep learning has rapidly been filtrating many aspects of human lives. In particular, image recognition by convolutional neural networks inspired numerous studies in this area. Hardware and software technologies as well large quantities data have contributed to the drastic development field. However, application deep is often hindered need for big laborious manual annotation thereof. To experience using compiled us, we collected 2429 constrained headshot images 277 volunteers. The collection...
Among pediatric renal tumors, rhabdoid tumor of the kidney (RTK) and clear cell sarcoma (CCSK) are rare associated with an unfavorable prognosis, while congenital mesoblastic nephroma (CMN) is a good prognosis. Methylation Ras association domain-containing protein 1 isoform A (RASSF1A) promoter has been reported to correlate poor prognosis in patients Wilms its methylation status unclear other types tumors.DNA RASSF1A several tumors was analyzed pyrosequencing. In order clarify correlation...
We have developed methods to functionalize polystyrene (PS) substrates with hydroxyapatite (HAp) via protein adsorption layers in simulated body fluid (SBF), a solution similar inorganic ion concentrations those of human plasma. In this study, three-dimensional (3D) porous HAp cell scaffolds were prepared by using PS as template. Human serum albumin was adsorbed on the 3D PS. Then pretreated alternate soaking process solutions containing calcium ions and phosphate followed incubation SBF. By...
<div>Abstract<p>Translocations of retinoic acid receptor-α (<i>RARA</i>), typically <i>PML–RARA</i>, are a genetic hallmark acute promyelocytic leukemia (APL). However, because small fraction APL lack translocations <i>RARA</i>, we focused here on cases without <i>RARA</i> translocation to elucidate the molecular etiology <i>RARA</i>-negative APL. We performed whole-genome sequencing, PCR, and FISH for five...
<p>Supplementary Methods: IRB approval, Genomic analysis, Identification of translocations and determination their breakpoints, Plasmids Construct, Cell line reagent, Co-immunoprecipotation immunoblotting Luciferase assay, Retrovirus production transduction, FCM Morphologic Human CB cell culture, Colony replating assay. Supplementary Tables: Table 1. Clinical characteristics RARA translocation negative APL cases. 2. BAC clones for FISH used in this study. 3. Primer sequences. 4....
<p>Supplementary Methods: IRB approval, Genomic analysis, Identification of translocations and determination their breakpoints, Plasmids Construct, Cell line reagent, Co-immunoprecipotation immunoblotting Luciferase assay, Retrovirus production transduction, FCM Morphologic Human CB cell culture, Colony replating assay. Supplementary Tables: Table 1. Clinical characteristics RARA translocation negative APL cases. 2. BAC clones for FISH used in this study. 3. Primer sequences. 4....
<div>Abstract<p>Translocations of retinoic acid receptor-α (<i>RARA</i>), typically <i>PML–RARA</i>, are a genetic hallmark acute promyelocytic leukemia (APL). However, because small fraction APL lack translocations <i>RARA</i>, we focused here on cases without <i>RARA</i> translocation to elucidate the molecular etiology <i>RARA</i>-negative APL. We performed whole-genome sequencing, PCR, and FISH for five...
Abstract Deep learning has rapidly been filtrating many aspects of human lives. In particular, image recognition by convolutional neural networks inspired numerous studies in this area. Hardware and software technologies as well large quantities data have contributed to the drastic development field. However, application deep is often hindered need for big laborious manual annotation thereof. To experience using compiled us, we collected 2429 constrained headshot images 277 volunteers. The...
Abstract BACKGROUND Genetic hallmark of atypical teratoid/rhabdoid tumor (AT/RT) is loss-of-function variants or deletions in SMARCB1 gene on 22q11.2 chromosome, which common to extracranial malignant rhabdoid tumors (MRT). Previous studies demonstrated that approximately one-thirds AT/RT and MRT patients harbored germline as the predisposing syndrome. We studied herein intensive analysis focusing prevalence genetic variants. PROCEDURE: In total, 16 were included. Both tumor-derived DNA...