A5064785088- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Synthesis of β-Lactam Compounds
- Antibiotics Pharmacokinetics and Efficacy
- Synthesis and Catalytic Reactions
- Genomics and Phylogenetic Studies
- Antifungal resistance and susceptibility
- Lipid metabolism and biosynthesis
- Bacterial biofilms and quorum sensing
- Enzyme Catalysis and Immobilization
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Mycobacterium research and diagnosis
- Enzyme function and inhibition
- Microbial Community Ecology and Physiology
- Amino Acid Enzymes and Metabolism
- Synthesis and Biological Evaluation
- Peptidase Inhibition and Analysis
- Polar Research and Ecology
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Pharmacogenetics and Drug Metabolism
- Veterinary medicine and infectious diseases
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
University of Liège
2014-2025
Bioénergétique et Ingénierie des Protéines
2011
ABSTRACT Gram-negative bacteria are a major concern for public health, particularly due to the continuous rise of antibiotic resistance. A factor that helps development resistance is outer membrane essential since it acts as strong permeability barrier many antibiotics effective against other bacteria. In this study, we determine specific coefficients various in Pseudomonas aeruginosa strains, which differ from each their porin expressions. We showed OprD and OpdP porins contribute both...
β-Lactam antibiotics have long been a treatment of choice for bacterial infections since they bind irreversibly to Penicillin-Binding Proteins (PBPs), enzymes that are vital cell wall biosynthesis. Many pathogens express drug-insensitive PBPs rendering β-lactams ineffective, revealing need new types PBP inhibitors active against resistant strains. We identified alkyl boronic acids including methicillin-resistant S. aureus (MRSA). The crystal structures PBP1b complexed 11 different boronates...
Background Penicillin-binding proteins (PBPs) are well known and validated targets for antibacterial therapy. The most important clinically used inhibitors of PBPs β-lactams inhibit transpeptidase activity by forming a covalent penicilloyl-enzyme complex that blocks the normal transpeptidation reaction; this finally results in bacterial death. In some resistant bacteria resistance is acquired active-site distortion PBPs, which lowers their acylation efficiency β-lactams. To address problem...
As a complement to the renowned bicyclic β-lactam antibiotics, monocyclic analogues provide breath of fresh air in battle against resistant bacteria. In that framework, present study discloses silico design and unprecedented ten-step synthesis eleven nocardicin-like enantiomerically pure 2-{3-[2-(2-aminothiazol-4-yl)-2-(methoxyimino)acetamido]-2-oxoazetidin-1-yl}acetic acids starting from serine as readily accessible precursor. The capability this novel class 3-amino-β-lactams inhibit...
Abstract Transcriptomes consist of several classes RNA that have wide-ranging but often poorly described functions and the deregulation which leads to numerous diseases. Engineering functionalized RNA-binding proteins (RBPs) could therefore many applications. Our previous studies suggested RanBP2-type Zinc Finger (ZF) domain is a suitable scaffold investigate design single-stranded RBPs. In present work, we analyzed natural sequence specificity various members ZF family characterized...
Bis-2-oxoazetidinyl macrocycles, obtained as unexpected products of RCM cyclizations, exhibit good activities against <sc>d</sc>,<sc>d</sc>-peptidase from <italic>Actinomadura</italic> R39 and revealed very promising PBP2a methicillin-resistant <italic>Staphylococcus aureus.</italic>
In the chapters dealing with enzyme reactions, authors of all Biochemistry textbooks and even more specialized texts consider that characteristic parameters (kcat Km) must be determined under initial or steady-state rate conditions. This implies transformation a very limited proportion substrate (at most 10-20%) continuous recording product concentration vs. time. Both options can present practical difficulties. Is it possible to get around these stringent conditions? Here we show in...
ABSTRACT Phormidesmis priestleyi ULC007 is an Antarctic freshwater cyanobacterium. Its draft genome 5,684,389 bp long. It contains a total of 5,604 protein-encoding genes, which 22.2% have no clear homologues in known genomes. To date, this the first one ever determined for axenic cyanobacterium from Antarctica.
The aim of this study is to characterize the factors related peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported increase IS256 transposition invasive hVISA clinical strains isolated from same patient (D1 and D2) before after vancomycin treatment two laboratory VISA mutants (D23C9 D2P11) selected D2 independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis muropeptides showed increased proportion monomeric a...
Innovative monocyclic β-lactam entities create opportunities in the battle against resistant bacteria because of their PBP acylation potential, intrinsically high β-lactamase stability and compact scaffold. α-Benzylidene-substituted 3-amino-1-carboxymethyl-β-lactams were recently shown to be potent inhibitors constitute eligible anchor points for synthetic elaboration chemical space around central ring. The present study discloses a 12-step synthesis ten α-arylmethylidenecarboxylates using...
Abstract Monocyclic β‐lactams revive the research field on antibiotics, which are threatened by emergence of resistant bacteria. A six‐step synthetic route was developed, providing easy access to new 3‐amino‐1‐carboxymethyl‐4‐phenyl‐β‐lactams, penicillin‐binding protein (PBP) inhibitory potency demonstrated biochemically.
Infections with multidrug-resistant bacteria pose a major healthcare problem which urges the need for novel treatment options. Besides its potent antiplatelet properties, ticagrelor has antibacterial activity against Gram-positive bacteria, including methicillin- and vancomycin-resistant
When enzyme inhibition by either the product or excess substrate occurs, it is possible to determine characteristic kinetic parameters based on [P]/t measurements, even when a large proportion of converted. The advantages various approaches are discussed. Most them allow good estimation V and Km values. Conversely, determination Kp (product inhibition) Ki (inhibition substrate) can be more challenging. In first case, type requires complex experiments that beyond scope present contribution....
The study of the interactions between enzymes and inactivators can often be performed with help reporter substrate method in which time-dependent decrease rate disappearance (or product formation) is monitored. In present contribution, we wish to describe examples utilization this rapid efficient for reactions whose rates monitored by spectrophotometric or fluorimetric measurements. After collection data an Excel file, a very simple program applied extract values pseudo-- first-order...
Fungi are of great importance in biotechnology, for example the production enzymes and metabolites. The main goal this study was to obtain a high-coverage draft Stachybotrys microspora genome annotate analyze sequence data. rare fungus S. N1 strain is distinguished by its ability grow an alkaline halophilic environment efficiently secrete cellulolytic enzymes. Here we report composed 3715 contigs, size 35 343 854 bp, with GC content 53.31% coverage around 20.5×. identification genes their...