A5071298407- Axon Guidance and Neuronal Signaling
- Glycosylation and Glycoproteins Research
- Embedded Systems Design Techniques
- Hippo pathway signaling and YAP/TAZ
- VLSI and FPGA Design Techniques
- Parallel Computing and Optimization Techniques
- Wind and Air Flow Studies
- Neurogenesis and neuroplasticity mechanisms
- Galectins and Cancer Biology
- VLSI and Analog Circuit Testing
- Microtubule and mitosis dynamics
- Attention Deficit Hyperactivity Disorder
- Hereditary Neurological Disorders
- Interconnection Networks and Systems
- Ubiquitin and proteasome pathways
- Fluid Dynamics and Vibration Analysis
- Pain Mechanisms and Treatments
- Low-power high-performance VLSI design
- Connective tissue disorders research
- Protein Tyrosine Phosphatases
- Neurological diseases and metabolism
- Hedgehog Signaling Pathway Studies
- Wnt/β-catenin signaling in development and cancer
- Natural Language Processing Techniques
- Neuroscience and Neuropharmacology Research
Osaka University
2018-2025
Child Development Center
2018-2025
Baylor College of Medicine
2014-2022
NTT (Japan)
2002-2021
National Institutes of Natural Sciences
2012-2020
National Institute for Physiological Sciences
2012-2020
The Graduate University for Advanced Studies, SOKENDAI
2012-2020
Department of Physiological Sciences
2017-2018
Waseda University
2004-2015
Asahi Kasei (Japan)
2011-2012
Collapsin response mediator protein 2 (CRMP-2) enhances the advance of growth cones by regulating microtubule assembly and Numb-mediated endocytosis. We previously showed that Rho kinase phosphorylates CRMP-2 during cone collapse; however, roles phosphorylated in collapse remain to be clarified. Here, we report phosphorylation cancels binding activity tubulin dimer, microtubules, or Numb. binds actin, but its is not affected phosphorylation. Electron microscopy revealed localizes on...
A neuron has two types of highly polarized cell processes, the single axon and multiple dendrites.One fundamental questions neurobiology is how neurons acquire such specific morphologies.During neuronal development, various actin-binding proteins regulate dynamics actin cytoskeleton in growth cones developing axons.The regulation thought to be involved outgrowth axon-dendrite specification.However, it largely unknown which actinbinding are specification they transported into axons.We have...
Rac1 and Cdc42, members of the Rho family GTPases, control diverse cellular processes such as cell migration morphogenesis through their effectors. Among effectors, IQGAP1 plays pivotal roles in establishment cytoskeletal architecture intercellular adhesions various cells. However, its remain to be clarified, especially neuronal We have identified IQGAP3 a novel member IQGAP family, which is highly expressed brain. found that IQGAP3, an effector associates directly with actin filaments...
Semaphorin3A (Sema3A) is a repulsive guidance molecule for axons, which acts by inducing growth cone collapse through phosphorylation of CRMP2 (collapsin response mediator protein 2). Here, we show role oxidation and thioredoxin (TRX) in the regulation collapse. Sema3A stimulation generated hydrogen peroxide (H2O2) MICAL (molecule interacting with CasL) oxidized CRMP2, enabling it to form disulfide-linked homodimer cysteine-504. Oxidized then formed transient complex TRX, stimulated glycogen...
The correct balance between excitation and inhibition is crucial for brain function disrupted in several pathological conditions. Excitatory neuronal circuits the primary somatosensory cortex (S1) are modulated by local inhibitory neurons with of this excitatory activity important function. layer 2/3 (L2/3) S1 increased chronic pain, but it not known how interneurons, nor inhibition, may change pain. Using vivo two-photon calcium imaging electrophysiology, we report here that response L2/3...
Abstract Background Attention-deficit hyperactivity disorder (ADHD) is a neurodevelopmental (NDD) with three characteristics: hyperactivity, inattention, and impulsivity. Although the number of patients ADHD has increased in recent years, its pathology remains unclear. The axon initial segment (AIS) region proximal axon, which responsible for information output by generating action potentials. Recent studies have shown that structure AIS can be altered based on input to neuronal circuit,...
This paper describes a new hardware/software co-verification method for System-On-a-Chip, based on the integration of C/C++ simulator and an inexpensive FPGA emulator. Communication between emulator occurs via flexible interface shared communication registers. enables easy debugging, rich portability, high verification speed, at low cost. We describe application this environment to three different complex commercial SoCs, supporting concurrent hardware embedded software development. In these...
The polypeptide hormone calcitonin is clinically well known for its ability to relieve neuropathic pain such as spinal canal stenosis, diabetic neuropathy and complex regional syndrome. Mechanisms analgesic effect, however, remain unclear. Here we investigated the mechanism of anti-hyperalgesic action in a model rats.Subcutaneous injection elcatonin, synthetic derivative eel calcitonin, relieved hyperalgesia induced by chronic constriction injury (CCI). Real-time reverse...
Oligosaccharides of glycoproteins expressed on the cell surface play important roles in cell-cell interactions, particularly sialylated N-glycans having a negative charge, which interact with sialic acid-binding immunoglobulin-like lectins (siglecs). The entire structure mouse brain, linkage type acid residues attached to backbone N-glycans, has not yet been elucidated. An improved method analyze pyridylaminated sugar chains using high performance liquid chromatography (HPLC) was developed...
Axon initial segments (AIS) and nodes of Ranvier are highly specialized axonal membrane domains enriched in Na+ channels. These channel clusters play essential roles action potential initiation propagation. AIS nodal complexes linked to the actin cytoskeleton through βIV spectrin. However, neuronal spectrin exists as two main splice variants: a longer βIVΣ1 variant with canonical N-terminal αII spectrin-binding domains, shorter βIVΣ6 lacking these domains. Here, we show that predominant...
Astrocytes have recently been shown to provide physiological support for various brain functions, although little is known about their involvement in white matter integrity. Several inherited infantile-onset leukoencephalopathies, such as Alexander disease and megalencephalic leukoencephalopathy with subcortical cysts (MLC), implicate astrocytic the formation of matter. mouse models MLC had generated by knocking out Mlc1 gene; however, none those was reported show myelin abnormalities prior...
Highly specialized glial cells wrap axons with a multilayered myelin membrane in vertebrates. Myelin serves essential roles the functioning of nervous system. Axonal degeneration is major cause permanent neurological disability primary diseases. Many glycoproteins have been identified myelin, and lack one glycoprotein results abnormal structures many cases. However, glycans on remain poorly understood. Here, we report that sulfated N-glycans are involved peripheral system (PNS) myelination....
Proper N-glycosylation of proteins is important for normal brain development and nervous system function. Identification the localization, carrier interacting partners N-glycans essential understanding roles glycoproteins. The present study examined N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-). has a branched sialic acid structural feature, sialylated major in mouse brain. Its expression increases during development, suggesting that...