A5009743531- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion Channels and Receptors
- Nerve injury and regeneration
- Insect and Pesticide Research
- Genetics and Neurodevelopmental Disorders
- Animal Ecology and Behavior Studies
- Nicotinic Acetylcholine Receptors Study
- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- RNA regulation and disease
- Neuroscience and Neuropharmacology Research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Regulation of Appetite and Obesity
- Light effects on plants
- Molecular Communication and Nanonetworks
- Optical Imaging and Spectroscopy Techniques
- Blood Coagulation and Thrombosis Mechanisms
- Pain Mechanisms and Treatments
- Thermoregulation and physiological responses
- Protein Degradation and Inhibitors
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
Nagoya University
2020-2023
National Institute for Physiological Sciences
2011-2023
National Institutes of Natural Sciences
2012-2023
Kobe University
2019
Gunma University
2015-2018
The Graduate University for Advanced Studies, SOKENDAI
2010-2016
Okazaki Institute for Integrative Bioscience
2010
Kyushu Institute of Technology
2005
Kyushu University
1998
Optogenetics has been enthusiastically pursued in recent neuroscience research, and the causal relationship between neural activity behavior is becoming ever more accessible. Here, we established knockin-mediated enhanced gene expression by improved tetracycline-controlled induction (KENGE-tet) succeeded generating transgenic mice expressing a highly light-sensitive channelrhodopsin-2 mutant at levels sufficient to drive activities of multiple cell types. This method requires two lines mice:...
Abstract Maternal infection or inflammation causes abnormalities in brain development associated with subsequent cognitive impairment and an increased susceptibility to schizophrenia autism spectrum disorders. immune activation (MIA) increases serum cytokine levels mediates this association via effects on the fetal brain, microglia can respond maternal status, but consensus how may is lacking no-one has yet examined if microglial process motility impaired. In study we investigated MIA...
Abstract Myelination increases the conduction velocity in long‐range axons and is prerequisite for many brain functions. Impaired myelin regulation or impairment of itself frequently associated with deficits learning cognition neurological psychiatric disorders. However, it has not been revealed what perturbation neural activity induced by causes deficits. Here, we measured motor cortex during transgenic mice a subtle their myelin. This deficit impaired learning, was accompanied decrease...
Using region-specific injection of hyaluronic acid, we developed a mouse model acute retinal detachment (RD) to investigate molecular mechanisms photoreceptor cell death triggered by RD. We focused on the transient receptor potential vanilloid 4 (TRPV4) ion channel, which functions as thermosensor, osmosensor, and/or mechanosensor. After RD, number apoptotic photoreceptors was reduced ∼50% in TRPV4KO mice relative wild-type mice, indicating possible involvement TRPV4 activation RD-induced...
Serotonergic (5HT) neurons of the dorsal raphe nuclei receive excitatory input from hypothalamic orexin (hypocretin) and reciprocally inhibit through 5HT1A receptor. However, physiological significance this negative feedback circuit for sleep/wakefulness regulation is little understood.5HT1A receptor expression level was specifically reversibly controlled in using Tet-off system. The responsiveness to 5HT vitro patterns were compared between 5HT1A-overexpressing control mice.When...
We have previously reported that transient receptor potential vanilloid 2 (TRPV2) can be activated by mechanical stimulation, which enhances axonal outgrowth in developing neurons; however, the molecular mechanisms govern contribution of TRPV2 activation to remain unclear. In present study, we examined this mechanism using PC12 cells as a neuronal model. Overexpression enhanced stimulus-dependent manner. Accumulation at cell surface was 4-fold greater growth cone compared with soma. cone, is...
Mature oligodendrocytes are critical for myelin maintenance. To understand the molecular basis this, genetic manipulation of mature is needed. Here we generated a oligodendrocyte tTA (tetracycline-controlled transcriptional activator) mouse line which, in combination with tTA-dependent promoter driving expression desired transgene, can be used gain-of-function studies. We an promoter, proteolipid protein (PLP) to express mammalianized tTA, and PLP-mtTA line. In adults, mtTA mRNA was...
Astrocytes have recently been shown to provide physiological support for various brain functions, although little is known about their involvement in white matter integrity. Several inherited infantile-onset leukoencephalopathies, such as Alexander disease and megalencephalic leukoencephalopathy with subcortical cysts (MLC), implicate astrocytic the formation of matter. mouse models MLC had generated by knocking out Mlc1 gene; however, none those was reported show myelin abnormalities prior...
Abstract Neonicotinoid pesticides are a class of insecticides that reportedly have harmful effects on bees and dragonflies, causing reduction in their numbers. Neonicotinoids act as neuroreceptor modulators, some studies reported association with neurodevelopmental disorders. However, the precise effect neonicotinoids central nervous system has not yet been identified. Herein, we conducted vivo Ca 2+ imaging using two-photon microscope to detect abnormal activity neuronal circuits brain...
Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological of NN clothianidin (CLO) by vivo Ca2+ imaging using two-photon microscopy. Mice were fed no-observed-adverse-effect-level (NOAEL) dose CLO for 2 weeks and neuronal activity primary somatosensory cortex (S1) was observed weekly weeks. exposure caused a sustained influx neurons S1 2/3 layers, indicating hyperactivation...
Brain function relies on both rapid electrical communication in neural circuitry and appropriate patterns or synchrony of activity. Rapid between neurons is facilitated by wrapping nerve axons with insulation a myelin sheath composed largely different lipids. Recent evidence has indicated that the extent myelination can adapt based activity levels this adaptive associated improved learning motor tasks, suggesting such plasticity may enhance effective learning. In study, we examined whether...
Oligodendrocytes (OCs) form myelin around axons, which is dependent on neuronal activity. This activity-dependent myelination plays a crucial role in training and learning. Previous studies have suggested that activity regulates proliferation differentiation of oligodendrocyte precursor cells (OPCs) myelination. In addition, deficient results impaired motor However, the functional response OC responsible for their pathological changes not fully elucidated. this research, we aimed to...
Subcortical white matter infarction causes ischemic demyelination and loss of brain functions, as the result disturbances blood flow. Although angiogenesis is one recovery processes after cerebral infarction, dynamics revascularization still remains unclear. We induced in internal capsule Flk1-GFP::Flt1-tdsRed double transgenic mice by injection endothelin-1 (ET-1), a vasoconstrictor peptide, together with N(G)-nitro-L-arginine methyl ester (L-NAME), nitric oxide synthase inhibitor, followed...
Mlc1 is a causative gene for megalencephalic leukoencephalopathy with subcortical cysts, and expressed in astrocytes. Mlc1-over-expressing mice represent an animal model of early-onset leukoencephalopathy, which manifests as astrocytic swelling followed by myelin membrane splitting the white matter. It has been previously reported that highly Bergmann glia, while cerebellar phenotypes mouse have not characterized. Here, we examined cerebellum found distribution glia (BG) was normally...
The change in blood volume and flow the cortical tissue of guinea pigs caused by brain activation during auditory stimulation is measured with a multispectral imaging system laser speckle flowgraphy system.
Abstract Neonicotinoid pesticides are insecticides that reportedly have untargeted effects on bees and dragonflies causing a reduction in numbers. Neonicotinoids act as neuroreceptor modulators, some studies reported their association with neurodevelopmental disorders. However, the effect of neonicotinoids central nervous system has not yet been identified. Herein, we conducted vivo Ca 2+ imaging using two-photon microscope to detect abnormal activity neuronal circuits brain neonicotinoid....