A5011986003- Metabolomics and Mass Spectrometry Studies
- Osteoarthritis Treatment and Mechanisms
- Mass Spectrometry Techniques and Applications
- Total Knee Arthroplasty Outcomes
- Knee injuries and reconstruction techniques
- Analytical Chemistry and Chromatography
- Nerve injury and regeneration
- Mesenchymal stem cell research
- Neuropeptides and Animal Physiology
- Orthopedic Surgery and Rehabilitation
- Advanced Optical Imaging Technologies
- Dialysis and Renal Disease Management
- Chronic Kidney Disease and Diabetes
- Muscle activation and electromyography studies
- Neuroendocrine regulation and behavior
- Photorefractive and Nonlinear Optics
- Sports Performance and Training
- Muscle and Compartmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Barrier Structure and Function Studies
- Advanced Proteomics Techniques and Applications
- Wildlife Ecology and Conservation
- Periodontal Regeneration and Treatments
- Lower Extremity Biomechanics and Pathologies
- Orthopaedic implants and arthroplasty
Hiroshima University
2024-2025
Hamamatsu University School of Medicine
2021-2024
Tokyo National Hospital
2024
Komazawa University
2024
University of Fukui
1983-2023
Morinomiya University of Medical Sciences
2023
Tokyo Institute of Technology
2019-2022
Kumamoto University
2021
Kure Medical Center
2021
National Hospital Organization
2021
CD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for or cartilage repair. However, the nature CD271+ from adipose tissue (AT) less well understood. Here, we investigated differentiation, wound healing and angiogenic plastic adherent (PA MSCs) versus AT. There was no difference in extent to which PA formed osteoblasts, adipocytes chondrocytes vitro. In contrast, transplanted into athymic rats significantly osteochondral reduced vascularisation repair compared...
The blood-brain barrier (BBB) is composed of four cell populations, brain endothelial cells (BECs), pericytes, neurons, and astrocytes. Its role to precisely regulate the microenvironment through selective substance crossing. Here we generated an in vitro model BBB by differentiating human induced pluripotent stem (hiPSCs) into all populations. When hiPSC-derived populations were co-cultured, (ECs) endowed with features consistent BECs, including a high expression nutrient transporters...
Mass spectrometry imaging (MSI) is well-known for the non-labeling visualization of analytes, including drugs and their metabolites in biological samples. In this study, we applied three different tools MSI, desorption electrospray ionization (DESI)-MSI, matrix-assisted laser (MALDI)-MSI, a newly developed atmospheric pressure (AP)-MALDI-MSI known as iMScopeTM QT rapid mapping imipramine, chloroquine, C57BL/6 male wild-type mice. Among MSI tools, better detection capability targeted at...
Drug distribution studies in tissue are crucial for understanding the pharmacokinetics and potential toxicity of drugs. Recently, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has gained attention drug due to its high sensitivity, label-free nature, ability distinguish between parent drugs, their metabolites, endogenous molecules. Despite these advantages, achieving spatial resolution is challenging. Importantly, many drugs metabolites rarely detectable by...
Cancer tissues reflect a greater number of pathological characteristics cancer compared to cells, so the evaluation can be effective in determining treatment strategies. Mass spectrometry imaging (MSI) evaluate and even identify molecules while preserving spatial information. Cluster analysis tissues’ MSI data is currently used phenotype heterogeneity tissues. Interestingly, it has been reported that does not always coincide with genotype HER2-positive breast cancer. We thus investigated...
Low lumbar osteoporotic vertebral collapse (OVC) has not been well documented compared with OVC of the thoracolumbar spine. The differences between low and lesions should be studied to provide better treatment. aim this study was clarify clinical imaging features as outcomes discuss appropriate surgical treatment.Thirty patients (10 men; 20 women; mean age 79.3 ± 4.7 years [range 70-88 years]) affecting levels below L-3 underwent symptoms, morphological affected vertebra, sagittal...
Tissue clearing methods combined with confocal microscopy have been widely used for studying developmental biology. In plants, ClearSee is a reliable method that applicable to wide range of tissues and suitable gene expression analysis using fluorescent reporters, but its application the Arabidopsis thaliana embryo, model system study morphogenesis pattern formation, has not described in original literature. Here, we describe ClearSee-based protocol which obtaining 3D images embryos. The...
Abstract Purpose This study aimed to clarify the dosimetric impact of respiratory motion liver on stereotactic body radiation therapy (SBRT) for spine metastasis and examine utility introducing beam avoidance (beam‐off at specific gantry angles). Methods A total 112 consecutive patients who underwent SBRT between 2018 2024 were examined. Overall, 15 had lesions near dome included in this study. Retrospective treatment plans generated using computed tomography (CT) images acquired during...
Mass spectrometry imaging (MSI) allows us to visualize the spatial distribution of molecular components in a sample. A large amount mass data comprehensively provides distributions. In this study, we focus on information obtained and use Shannon entropy as quantity analyze MSI data. By calculating at each pixel sample, is from We found that low-entropy pixels heat maps for kidneys mice had different structures between two ages (3 months 31 months). Such changes cannot be visualized by...
Abstract Society in eusocial insects is based on the reproductive division of labor, with a small number individuals supported by large nonreproductive individuals. Because inclusive fitness all colony members depends survival and fertility members, sterile provide royals special treatment. Here, we show that termite kings queens each receive food different composition from workers. Sequential analysis feeding processes demonstrated workers exhibit discriminative trophallaxis, indicating...
Brain function relies on both rapid electrical communication in neural circuitry and appropriate patterns or synchrony of activity. Rapid between neurons is facilitated by wrapping nerve axons with insulation a myelin sheath composed largely different lipids. Recent evidence has indicated that the extent myelination can adapt based activity levels this adaptive associated improved learning motor tasks, suggesting such plasticity may enhance effective learning. In study, we examined whether...
Abstract In patients with unresectable non-small cell lung cancer, histological diagnosis is frequently based on small biopsy specimens unsuitable for when they are severely crushed and do not retain their morphology. Therefore, establishing a novel diagnostic method independent of tissue morphology or conventional immunohistochemistry (IHC) markers required. We analyzed the lipid profiles resected primary adenocarcinoma (ADC) squamous carcinoma (SQCC) using liquid chromatography-tandem mass...
Ninjin'yoeito (NYT), a Japanese traditional Kampo medicine, has been reported to exert various clinical benefits such as relief from fatigue, malaise, anorexia, frailty, sarcopenia, and cognitive dysfunction. Recently, some review articles described the pharmacological effects of NYT additionally indicated possibility that multiple ingredients in contribute these effects. However, pharmacokinetic data on are essential addition their activities accurately determine active NYT.This study...
Knowledge of gender-specific drug distributions in different organs are great importance for personalized medicine and reducing toxicity. However, such have not been well studied. In this study, we investigated potential differences the distribution imipramine chloroquine, as their metabolites, between male female kidneys. Kidneys were collected from mice treated with or chloroquine then subjected to atmospheric pressure matrix-assisted laser desorption ionization-mass spectrometry imaging...
Abstract Background Buerger disease, also known as Winiwarter–Buerger disease or thromboangiitis obliterans (TAO), is a non-specific inflammation of small- and medium-sized arteries with thrombus obliteration without atherosclerotic changes. Patients TAO can develop chronic limb-threatening ischaemia (CLTI) are at risk limb amputation despite smoking cessation exercise therapy recommendations. Case summary A 72-year-old Japanese man presented painful discolouration toes renal impairment. He...
We have investigated the alignment of nematic liquid crystals (NLCs) using 50 nm ultrafine nanogrooves fabricated by electron-beam (EB) lithography and nanoimprint lithography. To improve wettability NLCs, silicon dioxide was sputtered onto NLCs after nanogroove fabrication. The azimuthal polar anchoring energies obtained were 9.4×10−5 J/m2 3.6×10−4 J/m2, respectively. From electro-optical characteristic a twisted (TN) crystal (LC) cell, contrast ratio TN cell found to be 44:1, which is...
Previous basic research and clinical studies examined the effects of mesenchymal stem cells (MSCs) on regeneration maintenance articular cartilage. However, our pilot study suggested that MSCs are more effective at suppressing inflammation pain rather than promoting cartilage in osteoarthritis. Adipose tissue is considered a useful source MSCs; it can be harvested easily larger quantities compared with bone marrow. The present was designed to evaluate anti-inflammatory, analgesic,...
To understand the ultra-early reaction of normal organ lipids during irradiation, we investigated response lipids, including polyunsaturated fatty acid (PUFA) chains, which are particularly susceptible to damage by ROS, in mice’s kidneys, lungs, brains, and livers within 5 min single high-dose irradiation. In this study, set up three groups C56BL/6 male mice conducted whole-body irradiation with 0 Gy, 10 20 Gy doses. Kidney, lung, brain, liver tissues were collected PUFA-targeted whole...
Mass spectrometry imaging (MSI) is essential for visualizing drug distribution, metabolites, and significant biomolecules in pharmacokinetic studies. This study mainly focuses on imipramine, a tricyclic antidepressant that affects endogenous metabolite concentrations. The aim was to use atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI)-MSI combined with different dimensionality reduction methods examine the distribution impact of imipramine metabolites brains...