A5040654017- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Wnt/β-catenin signaling in development and cancer
- Laser Applications in Dentistry and Medicine
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Animal Genetics and Reproduction
- Coronary Interventions and Diagnostics
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Skin and Cellular Biology Research
- Chalcogenide Semiconductor Thin Films
- Neuroscience and Neural Engineering
- Laser Material Processing Techniques
- Quantum Dots Synthesis And Properties
- Biomedical Ethics and Regulation
- Plant and animal studies
- RNA Research and Splicing
- Ocular and Laser Science Research
- Laser Design and Applications
- Copper-based nanomaterials and applications
- Photoacoustic and Ultrasonic Imaging
Kyoto University
2015-2025
Tokyo University of Information Sciences
2023
The University of Tokyo
2022-2023
Ritsumeikan University
2022
Nagahama City Hospital
2015-2021
Nara Medical University
1989-2020
Institute for Biological Sciences
2020
Tokushima University
2017
Italian Aerospace Research Centre
2015-2017
Nagaoka National College of Technology
2017
Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic by introducing Oct3/4 Sox2 with either Klf4 c-Myc or Nanog Lin28 using retroviruses lentiviruses. Patient-specific iPS could be useful in drug discovery regenerative medicine. However, viral integration into the host genome increases risk of tumorigenicity. Here, we report generation without vectors. Repeated transfection two expression plasmids, one containing complementary DNAs (cDNAs) Oct3/4, Sox2, other...
Induced pluripotent stem (iPS) cells have been generated from mouse and human fibroblasts by the retroviral transduction of four transcription factors. However, cell origins molecular mechanisms iPS induction remain elusive. This report describes generation adult hepatocytes gastric epithelial cells. These clones appear to be equivalent embryonic in gene expression are competent generate germline chimeras. Genetic lineage tracings show that liver-derived derived albumin-expressing No common...
In order to apply human embryonic stem cells (hESCs) and induced pluripotent (hiPSCs) regenerative medicine, the should be produced under restricted conditions conforming GMP guidelines. Since conventional culture system has some issues that need addressed achieve this goal, we developed a novel system. We found recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs hiPSCs when used in combination with completely xeno-free (Xf) medium, StemFit™. Using system, can...
The small guanosine triphosphatases (GTPases) Cdc42 and Rac1 regulate E-cadherin-mediated cell-cell adhesion. IQGAP1, a target of Rac1, was localized with E-cadherin beta-catenin at sites contact in mouse L fibroblasts expressing (EL cells), interacted both vivo vitro. IQGAP1 induced the dissociation alpha-catenin from cadherin-catenin complex vitro vivo. Overexpression EL cells, but not cells an E-cadherin-alpha-catenin chimeric protein, resulted decrease adhesive activity. Thus, acting...
Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy Parkinson's disease. However, iPSC-derived donor inevitably contain tumorigenic or inappropriate cells. Here, we show that human DA progenitor be efficiently isolated by sorting using floor plate marker, CORIN. We scalable culture conditions on laminin fragment, and the sorted CORIN(+) expressed markers, FOXA2 LMX1A. When transplanted into...
Induced pluripotent stem cells (iPSCs) are generated from mouse and human fibroblasts by the introduction of three transcription factors: Oct3/4, Sox2, Klf4. The proto-oncogene product c-Myc markedly promotes iPSC generation, but also increases tumor formation in iPSC-derived chimeric mice. We report that promotion generation Myc is independent its transformation property. found another family member, L-Myc, as well mutants (W136E dN2), all which have little activity, promoted more...
Highlights•Rapid, robust neuronal induction from human iPSCs to model AD drug responsiveness•iPSC-based screening of pharmaceutical compounds for Aβ phenotypes•A combination existing drugs synergistically improve phenotypes AD•Anti-Aβ cocktail decreases toxic levels in neurons derived patients' cellsSummaryIn the process development, vitro studies do not always adequately predict human-specific responsiveness clinical trials. Here, we applied advantage iPSC-derived neurons, which offer...
ABSTRACT Rac1, a member of the Rho family small GTPases, regulates E-cadherin-mediated cell-cell adhesion. However, it remains to be clarified how localization and activation Rac1 are regulated at sites contact. Here, using enhanced green fluorescence protein (EGFP)-tagged we demonstrate that EGFP-Rac1 is colocalized with E-cadherin contact translocates cytosol during disruption adhesion by Ca2+ chelation. Re-establishment restoration caused become relocalized, together E-cadherin,...
Human artificial chromosome (HAC) has several advantages as a gene therapy vector, including stable episomal maintenance that avoids insertional mutations and the ability to carry large inserts regulatory elements. Induced pluripotent stem (iPS) cells have great potential for therapy, such can be generated from individual's own tissues, when reintroduced contribute specialized function of any tissue. As proof concept, we show herein complete correction genetic deficiency in iPS derived...
SIRT1 is increasingly recognized as a critical regulator of stress responses, replicative senescence, inflammation, metabolism, and aging. expression regulated transcriptionally post-transcriptionally, its enzymatic activity controlled by NAD+ levels interacting proteins. We found that protein were much higher in mouse embryonic stem cells (mESCs) than differentiated tissues. miRNAs post-transcriptionally downregulated during mESC differentiation maintained low Specifically, miR-181a b,...
IQGAP1, a target of Cdc42 and Rac1 small GTPases, directly interacts with β-catenin negatively regulates E-cadherin-mediated cell-cell adhesion by dissociating α-catenin from the cadherin-catenin complex <i>in vivo</i> (Kuroda, S., Fukata, M., Nakagawa, Fujii, K., Nakamura, T., Ookubo, Izawa, I., Nagase, Nomura, N., Tani, H., Shoji, Matsuura, Y., Yonehara, Kaibuchi, K. (1998) <i>Science</i> 281, 832–835). Here we investigated how regulate IQGAP1 function. interacted amino-terminal region...
Neural crest cells (NCCs) are an embryonic migratory cell population with the ability to differentiate into a wide variety of types that contribute craniofacial skeleton, cornea, peripheral nervous system, and skin pigmentation. This suggests promising role NCCs as source for cell-based therapy. Although several methods have been used induce human (hNCCs) from pluripotent stem (hPSCs), such (ESCs) induced (iPSCs), further modifications required improve robustness, efficacy, simplicity these...