A5011567304- Hydraulic and Pneumatic Systems
- Flow Measurement and Analysis
- Vibration and Dynamic Analysis
- Nuclear Engineering Thermal-Hydraulics
- Advanced Sensor Technologies Research
- Bone Metabolism and Diseases
- Engineering Applied Research
- Electrical and Bioimpedance Tomography
- Advanced Sensor and Control Systems
- Water Systems and Optimization
- Nuclear reactor physics and engineering
- Industrial Technology and Control Systems
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Hydraulic flow and structures
- Fluid Dynamics and Vibration Analysis
- Fluid Dynamics and Mixing
- Heat transfer and supercritical fluids
- Orthopaedic implants and arthroplasty
- Cyclone Separators and Fluid Dynamics
- Non-Destructive Testing Techniques
- Nuclear Materials and Properties
- Biomedical Ethics and Regulation
- Metallurgical Processes and Thermodynamics
- CRISPR and Genetic Engineering
Gifu University
2005-2021
TEPCO (Japan)
2005-2015
Osaka University
2006-2011
University of Tsukuba
2011
National Center For Child Health and Development
2008
Japan Science and Technology Agency
2008
Central Research Institute of Electric Power Industry
2006
Oceaneering International (United States)
2006
Tokyo University of Science
2002
The University of Tokyo
2001
A cDNA library prepared from the mouse osteoblastic cell line MC3T3-E1 was screened for presence of specifically expressed genes by employing a combined subtraction hybridization/differential screening approach. identified and sequenced which encodes protein designated osteoblast-specific factor 2 (OSF-2) comprising 811 amino acids. OSF-2 has typical signal sequence, followed cysteine-rich domain, fourfold repeated domain C-terminal domain. The lacks transmembrane region. shows homology with...
Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (iPS) cells. However, many types are not easily accessible minimally invasive procedures. Here we evaluated dental pulp (DPCs) as an optimal source iPS cells, since they obtained from extracted teeth and be expanded under simple culture conditions. From all 6 DPC lines tested with the conventional 3 or 4 reprogramming factors, were effectively established 5 lines. Furthermore, determination...
Abstract Notch is a transmembrane protein that plays critical role in the determination of cellular differentiation pathways. Although its importance development mesenchymal tissues has been suggested, skeletal not well investigated. Northern blot experiments showed expression Notch1 MC3T3-E1 osteoblastic cells at early stages. When cytoplasmic domain (Notch-IC [NIC]) delivered by an adenovirus vector was expressed cells, significant increase calcified nodule formation observed long-term...
In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. this present study, we characterized hDPSCs an earlier developmental stage to evaluate the potential usage of these for tissue-regenerative therapy. at crown-completed showed a higher proliferation rate than those later stage. When either group cultured in medium promoting differentiation toward osteo/odontoblastic lineage, both became alkaline-phosphatase-positive, produced calcified matrix, and...
Vitamin A metabolites such as all-trans-retinoic acid (all-trans-RA) affect several steps of metabolic processes in vertebrates. In the last few years, studies have shown effect RA on bone formation and metabolism. However, mechanisms its action still remain unclear, especially with respect to regulation cells. Therefore, this study was carried out clarify how regulates activity osteoclasts. Using a pit assay involving unfractionated cells, including osteoclasts obtained from rabbits, we...
Exosomes are small vesicles secreted from many cell types. Their biological effects largely depend on their cellular origin and the physiological state of originating cells. by mesenchymal stem cells exert therapeutic against multiple diseases may serve as potential alternatives to therapies. We previously established characterized human leukocyte antigen (HLA) haplotype homo (HHH) dental pulp (DPC) lines wisdom teeth. In this study, we aimed investigate effect local administration HHH-DPC...
Cell adhesion molecules are involved in a number of biological functions, such as cell survival, differentiation, tissue repair, and development. A novel molecule, POEM (preosteoblast epidermal growth factor-like repeat protein with meprin, A5 protein, receptor protein-tyrosine phosphatase μ domain), was isolated by reverse transcription-polymerase chain reaction using set degenerate primers designed after other known factor (EGF)-like motifs. From its structure, suggested to be molecule...
Under certain opening conditions (partial opening) of a steam control valve, the piping system in power plant occasionally experiences large vibrations. To understand valve instability that is responsible for such vibrations, detailed experiments and CFD calculations were performed. As result these investigations, it was found under middle-opening condition, complex three-dimensional (3D) flow structure (valve-attached flow) sets up region leading to high pressure on part body. this rotates...
Osteoclast formation from hemopoietic precursors has been shown to require the support of stromal cells in bone tissue. In this study, we demonstrated that hepatocyte growth factor (HGF) is one cell-derived molecules responsible for osteoclast-like cell formation. For our experiments, used a coculture system osteoclastic and activation which blast are cocultured with calvaria-derived MC3T3-G2/PA6 (PA6) on dentine slices presence 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. Addition anti-HGF...
Human dental pulp cells (hDPCs) are a promising resource for regenerative medicine and tissue engineering can be used derivation of induced pluripotent stem (iPSCs). However, current protocols use reagents animal origin (mainly fetal bovine serum, FBS) that carry the potential risk infectious diseases unwanted immunogenicity. Here, we report chemically defined protocol to isolate maintain growth differentiation hDPCs. hDPCs cultured under these conditions showed significantly less primary...
Abstract The reprogramming of somatic cells into induced pluripotent stem (iPSCs) by defined transcription factors has been a well-established technique and will provide an invaluable resource for regenerative medicine. However, the low efficiency human iPSC is still limitation clinical application. Here we showed that potential dental pulp (DPCs) obtained from immature teeth much higher than those mature DPCs. Furthermore, DPCs can be reprogrammed OCT3/4 SOX2, conversely these two are...
Hypoxia enhances the reprogramming efficiency of human dermal fibroblasts to become induced pluripotent stem cells (iPSCs). Because we showed previously that hypoxia facilitates isolation and maintenance dental pulp (DPCs), examined here whether it promotes DPCs iPSCs. Unlike fibroblasts, early transient (3% O 2 ) transition iPSCs by 3.3- 5.1-fold compared with normoxia (21% ). The resulting closely resembled embryonic as well generated in normoxia, judged morphology expression cell markers....
The velocity profile of the flow in a pipe and its influence on factor used with conventional meters were investigated ultrasonic pulse Doppler measurements. From measured profiles, influences surface roughness Reynolds number characterized qualitatively quantitatively. As changes during plant operation, changes, producing change factor. Variation also Experiments conducted at high temperature pressure to evaluate method for measuring nuclear cooling water. Helium gas bubbles provided...
Recent evidence indicates that osteopontin (Opn), one of the bone matrix proteins, plays an important role in attachment osteoclasts to matrix. Besides being elaborated by osteoblasts, this protein is also produced osteoclasts. The present study was performed examine effect calcitonin (CT) on Opn mRNA expression isolated rabbit and clarify second messenger signaling effect. Eel CT inhibited as well bone-resorbing activity caused a transient increase intracellular calcium followed sustained...