A5025699932- melanin and skin pigmentation
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
- Hair Growth and Disorders
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Skin Protection and Aging
- Cancer, Hypoxia, and Metabolism
- Developmental Biology and Gene Regulation
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Tendon Structure and Treatment
- Hedgehog Signaling Pathway Studies
- Photochromic and Fluorescence Chemistry
- Mast cells and histamine
- Epigenetics and DNA Methylation
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- RNA Research and Splicing
- Pancreatic and Hepatic Oncology Research
- Mesenchymal stem cell research
- Connexins and lens biology
Gifu University
2016-2025
University of Occupational and Environmental Health Japan
2020-2021
RIKEN Center for Integrative Medical Sciences
2008
Ehime University
2008
Suzuki (Japan)
2008
Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (iPS) cells. However, many types are not easily accessible minimally invasive procedures. Here we evaluated dental pulp (DPCs) as an optimal source iPS cells, since they obtained from extracted teeth and be expanded under simple culture conditions. From all 6 DPC lines tested with the conventional 3 or 4 reprogramming factors, were effectively established 5 lines. Furthermore, determination...
A cancer stem cell population in malignant brain tumors takes an essential part tumor initiation, growth, and recurrence. Growth factors, such as epidermal growth factor, fibroblast factor-2, vascular endothelial platelet-derived hepatocyte are shown to support the proliferation of neural cells also may play key roles gliomagenesis. However, responsible factor(s), which controls maintenance cells, is not yet uncovered. We have established three lines from human gliomas. These were...
There is a gradient of β-catenin expression along the colonic crypt axis with highest levels at bottom. In addition, colorectal cancers show heterogeneous subcellular pattern accumulation. However, it remains unclear whether different Wnt signalling exert distinct roles in epithelium. Here, we investigated dose-dependent effect canonical activation on epithelial differentiation by controlling stabilised using doxycycline-inducible transgenic system mice. We that elevated induce amplification...
Unlike the thoroughly investigated melanocyte population in hair follicle of epidermis, growth and differentiation requirements melanocytes eye, harderian gland inner ear - so-called non-cutaneous remain unclear. In this study, we vitro vivo effects factors that regulate development on stem cells or precursors these melanocytes. general, a reduction KIT receptor tyrosine kinase signaling leads to disordered development. However, were revealed be less sensitive than cutaneous Instead, eye...
Abstract Tendons are dense fibrous structures that attach muscles to bones. Healing of tendon injuries is a clinical challenge owing poor regenerative potential and scarring. Here, we created reporter mice express EGFP, driven by the promoter tendon-specific Scleraxis ( Scx ) transcription-factor gene; then generated induced pluripotent stem cells (iPSCs) from these mice. Utilising fluorescently labelled iPSCs, developed tenogenic differentiation protocol. The iPSC-derived EGFP-positive...
Abstract Neural crest cells migrate throughout the embryo and differentiate into diverse derivatives: peripheral neurons, cranial mesenchymal cells, melanocytes. Because neural have critical roles in organogenesis, detailed elucidation of cell differentiation is important developmental biology. We recently reported that melanocytes could be induced from mouse ESCs. Here, we improved culture system showed existence crest-like precursors. The addition retinoic acid to medium reduced...
Rest (RE1-silencing transcription factor, also called Nrsf) is involved in the maintenance of undifferentiated state neuronal stem/progenitor cells vitro by preventing precocious expression genes. However, function during neurogenesis vivo remains to be elucidated because early embryonic lethal phenotype conventional knockout mice. In present study, we have generated conditional mice, which allow effect genetic ablation examined vivo. We show that plays a role suppressing genes cultured...
Summary The panda mutant in Japanese quail ( Coturnix japonica ) displays spots of wild‐type plumage on a white background and is controlled by an autosomal recessive allele s ). dotted third dw the locus with / having less pigmentation than quail. We mapped to chromosome 4 (CJA04) previous study. orthologous region chicken Gallus gallus genome includes endothelin receptor B2 EDNRB2 ), avian‐specific paralog B EDNRB mutations mammals retard migration neural crest cells (NCCs), which results...
Clear cell sarcoma (CCS) is an aggressive soft tissue malignant tumor characterized by a unique t(12;22) translocation that leads to the expression of chimeric EWS/ATF1 fusion gene. However, little known about mechanisms underlying involvement in CCS development. In addition, cellular origins have not been determined. Here, we generated EWS/ATF1-inducible mice and examined effects adult somatic cells. We found forced resulted development EWS/ATF1-dependent sarcomas mice. The histology...
Direct conversion is an innovative new technology that involves the of somatic cells to target without passing through a pluripotent state. Forced expression alone or in combination with transcription factors (TFs), which are critical for generation cells, important successful direct conversion. However, most unable directly convert into even forced expression. We herein demonstrated epithelial-mesenchymal transition (EMT) advantageous cells. previously reported mouse keratinocytes converted...
Tendon injuries are frequently occurring disorders; it is clinically important to enhance tendon regeneration and prevent functional impairment post-injury. While in children heal quickly with minimal scarring, those adults slowly accompanied by fibrotic scarring. Therefore, investigating the healing mechanisms after injury, identifying factors that regulate inherent regenerative capacity of tendons promising approaches promoting regeneration. Here, we identify PI3K-Akt signalling pathway...
In vivo transplantation of undifferentiated embryonic stem (ES) cells can produce teratomas with uncontrolled cell proliferation. Although ES may be attractive candidates for human cell-replacement therapy in the future, major limitation its application to is teratoma formation. present study, containing herpes simplex virus-thymidine kinase (HSV-tk) transgene a suicide gene expression under control Oct-4 promoter was used ablation cells, which teratomas, using three-dimensional culture...
Abstract Melanoblasts, precursor of melanocytes, are generated from the neural crest and differentiate into melanocytes during their migration throughout entire body. The melanoblasts thought to be progenitor cells that only melanocyte. Here, we show melanoblasts, even after they have already migrated skin, multipotent, being able generate neurons, glial cells, smooth muscle in addition melanocytes. We isolated Kit-positive CD45-negative (Kit+/CD45−) both embryonic neonate skin by flow...
Human dental pulp cells (hDPCs) are a promising resource for regenerative medicine and tissue engineering can be used derivation of induced pluripotent stem (iPSCs). However, current protocols use reagents animal origin (mainly fetal bovine serum, FBS) that carry the potential risk infectious diseases unwanted immunogenicity. Here, we report chemically defined protocol to isolate maintain growth differentiation hDPCs. hDPCs cultured under these conditions showed significantly less primary...
Abstract Epidermal melanocyte precursors migrate into developing hair follicles to form the stem cell system required supply pigmented melanocytes necessary for pigmentation in repetitive cycles. Hair graying is caused by irreversible defects self‐renewal and/or development of follicular cells follicles. To investigate mechanism(s) during normal aging process, we established a model mice repeatedly plucking or shaving trunk hairs. We plucked shaved hairs induce and accelerate counted gray By...