A5080339510- Alzheimer's disease research and treatments
- Tendon Structure and Treatment
- RNA Research and Splicing
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Exercise and Physiological Responses
- Cellular transport and secretion
- Biochemical effects in animals
- Monoclonal and Polyclonal Antibodies Research
- Polyamine Metabolism and Applications
- Microplastics and Plastic Pollution
- Microtubule and mitosis dynamics
- Helicobacter pylori-related gastroenterology studies
- biodegradable polymer synthesis and properties
- Inflammatory mediators and NSAID effects
- dental development and anomalies
- Circular RNAs in diseases
- Intensive Care Unit Cognitive Disorders
Institute of Science Tokyo
2024-2025
Tokyo Medical and Dental University
2016-2024
Keio University
2023
The University of Tokyo
1974-2022
Hokkaido University
1995-2015
Neurological Surgery
2015
Nippon Medical School
2015
Jikei University School of Medicine
2015
Shiga University of Medical Science
2015
Kyorin University
2015
Amyloid-β peptide (Aβ) plays an important role in the pathogenesis of Alzheimer's disease (AD). Aβ is generated by proteolysis β-amyloid precursor protein (APP) and cleared enzyme-mediated degradation phagocytosis microglia astrocytes. Some cytokines, such as TGF-β1, stimulate this phagocytosis. In contrast, cellular upregulation HSP70 expression provides cytoprotection against Aβ. activity relation to inhibition oligomerization stimulation has also been reported. Although these vitro...
Osteoarthritis (OA), the most common aging-related joint disease, is caused by an imbalance between extracellular matrix synthesis and degradation. Here, we discover that both strands of microRNA-455 (miR-455), -5p -3p, are up-regulated Sox9, essential transcription factor for cartilage differentiation function. Both miR-455-5p -3p highly expressed in human chondrocytes from normal articular mouse primary chondrocytes. We generate miR-455 knockout mice, find degeneration mimicking OA...
Modulation of ionic channel properties and synaptic functions by neurotransmitters hormones is called neuro-modulation may be the basis for many long-lasting changes in animal behavior, example, arousal or motivational states. We have previously shown a teleost, dwarf gourami, that terminal nerve (TN) cells are major component gonadotropin-releasing hormone (GnRH) system structurally independent from preoptic/hypophysial-GnRH system, which projects to pituitary facilitates gonadotropin...
Alzheimer's β-amyloid precursor protein (APP) associates with kinesin-1 via JNK-interacting 1 (JIP1); however, the role of JIP1 in APP transport by neurons remains unclear. We performed a quantitative analysis to understand axonal transport. In JIP1-deficient neurons, we find that both fast velocity (∼2.7 μm/s) and high frequency (66%) anterograde cargo are impaired reduced (∼1.83 lower (45%). identified two novel elements linked function, located central region JIP1b, interact coiled-coil...
<h3>Objectives:</h3> The aim of this study was to identify risk factors for rupture unruptured cerebral aneurysms (UCAs) in elderly Japanese patients aged 70 years or older. <h3>Methods:</h3> participants included all age older 3 prospective studies Japan (the Unruptured Cerebral Aneurysm Study [UCAS Japan], UCAS II, and the at Jikei University School Medicine). A total 1,896 with 2,227 UCAs were investigated. median mean follow-up periods 990 802.7 days, respectively. <h3>Results:</h3>...
Mechanoforces experienced by an organ are translated into biological information for cellular sensing and response. In mammals, the tendon connective tissue experiences resists physical forces, with tendon-specific mesenchymal cells called tenocytes orchestrating extracellular matrix (ECM) turnover. We show that Mohawk (Mkx), a transcription factor, is essential in mechanoresponsive tenogenesis through regulation of its downstream ECM genes such as type I collagens proteoglycans fibromodulin...
Tendons and ligaments are essential connective tissues that connect the muscle bone. Their recovery from injuries is known to be poor, highlighting crucial need for an effective therapy. A few reports have described development of artificial with sufficient strength human cells. In this study, we successfully generated a tendon-like tissue (bio-tendon) using induced pluripotent stem cells (iPSCs). We first differentiated iPSCs into mesenchymal (iPSC-MSCs) transfected them Mohawk (Mkx) obtain...
Amyloid-β peptide (Aβ) plays an important role in the pathogenesis of Alzheimer's disease (AD). Aβ is generated by secretase-mediated proteolysis β-amyloid precursor protein (APP), and cleared enzyme-mediated degradation phagocytosis. Transforming growth factor (TGF)-β1 stimulates this We recently reported that APP23 mouse model for AD showed fewer AD-related phenotypes when these animals were crossed with transgenic mice expressing heat shock (HSP) 70. here examined effect...
Tendons and ligaments are crucial connective tissues linking bones muscles, yet achieving full functional recovery after injury remains challenging. We investigated the characteristics of tendon stem/progenitor cells (TSPCs) by focusing on declining repair capacity with growth. Using single-cell RNA sequencing Achilles from 2- 6-week-old mice, we identified Cd55 Cd248 as novel surface antigen markers for TSPCs. Combining single-nucleus ATAC analyses revealed that positive fractions in tissue...
Tendons and ligaments are crucial connective tissues linking bones muscles, yet achieving full functional recovery after injury remains challenging. We investigated the characteristics of tendon stem/progenitor cells (TSPCs) by focusing on declining repair capacity with growth. Using single-cell RNA sequencing Achilles from 2- 6-week-old mice, we identified Cd55 Cd248 as novel surface antigen markers for TSPCs. Combining single-nucleus ATAC analyses revealed that positive fractions in tissue...
Tendon injuries are frequently occurring disorders; it is clinically important to enhance tendon regeneration and prevent functional impairment post-injury. While in children heal quickly with minimal scarring, those adults slowly accompanied by fibrotic scarring. Therefore, investigating the healing mechanisms after injury, identifying factors that regulate inherent regenerative capacity of tendons promising approaches promoting regeneration. Here, we identify PI3K-Akt signalling pathway...
Understanding protein subcellular localization and its dynamic changes is crucial for elucidating cellular function disease mechanisms, particularly under stress conditions, where can modulate responses. Currently available databases provide insights into steady-state conditions; however, stress-related remain poorly understood. Here, we present the Localizatome, a comprehensive database that captures stress-induced dynamics in living cells. Using an original high-throughput microscopy...
J. Neurochem. (2012) 120 , 795–805. Abstract Amyloid‐β peptide (Aβ), which is generated by the β‐ and γ‐secretase‐mediated proteolysis of β‐amyloid precursor protein (APP), plays an important role in pathogenesis Alzheimer’s disease (AD). We recently reported that prostaglandin E 2 (PGE ) stimulates production Aβ through both EP 4 receptors activation receptor endocytosis γ‐secretase. here found transgenic mice expressing mutant APP (APP23) showed a greater or lesser apparent cognitive...
X11-family proteins, including X11, X11-like (X11L) and 2 (X11L2), bind to the cytoplasmic domain of amyloid β-protein precursor (APP) regulate APP metabolism. Both X11 X11L are expressed specifically in brain, while X11L2 is ubiquitously. predominantly excitatory neurons, contrast which strongly inhibitory neurons. In vivo gene-knockout studies targeting X11L, or both, transgenic mice have reported that proteins suppress amyloidogenic processing endogenous mouse ectopic human with one...
MX is a strong direct acting mutagen on Salmonella typhimurium TA100 and present in chlorinated tap water which contains organic compounds. was administered orally to 7-week-old male F344 rats, its geno-toxicity the pyloric mucosa of stomach examined by analysis DNA single strand scissions alkaline elution method. The effect cell proliferation assays inductions replicative synthesis ornithine decarboxylase. at closes 20-48 mg/kg body weight induced dose-dependently (p&lt;0.02) 2 h after...
Evidence for the natural occurrence of poly(adenosine diphosphate ribose) in vivo was obtained using a sensitive radioimmunoassay and glycohydrolase, which specifically hydrolyzes ribose). Calf thymus, liver, kidney, brain, pancreas spleen contained Naturally occurring calf thymus is composed molecules various chain lengths, like that synthesized by an vitro system. estimated to contain about 0.02 microgram/mg DNA
Amyloid β-precursor protein (APP) is primarily cleaved by α- or β-secretase to generate membrane-bound, C-terminal fragments (CTFs). In turn, CTFs are potentially subject a second, intramembrane cleavage γ-secretase, which active in lipid raft-like membrane microdomain. Mature APP (N- and O-glycosylated APP), the actual substrate of these secretases, phosphorylated at cytoplasmic residue Thr668 this phosphorylation changes overall conformation domain APP. We found that nonphosphorylated...
Calcium chloride (CaCl2 inhibits stimulation of replicative DNA synthesis (RDS) induced in the pyloric mucosa male Fischer 344 rats by sodium (NaCl), which is a tumor promoter glandular stomach. Administration 1 ml 3.3 M NaCl gastric intubation maximal 15-fold increase RDS 17 h; this had returned to control level 48 h. 20–400 mM CaCl2 h before administration resulted 60–100% inhibition within 4–48 was dose-dependent. The 400 also decreased histological damage surface epithelial cells NaCl....