A5085406316- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Computational Drug Discovery Methods
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Animal testing and alternatives
- Receptor Mechanisms and Signaling
- Gastric Cancer Management and Outcomes
- COVID-19 Clinical Research Studies
- Wnt/β-catenin signaling in development and cancer
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Effects and risks of endocrine disrupting chemicals
- CRISPR and Genetic Engineering
- Kruppel-like factors research
- Pluripotent Stem Cells Research
- Ferroptosis and cancer prognosis
- Lipid Membrane Structure and Behavior
- Radiomics and Machine Learning in Medical Imaging
- Mesenchymal stem cell research
National Institute of Health Sciences
2016-2025
Creative Research Enterprises (United States)
2015-2016
National Institute of Infectious Diseases
2016
Kobe University
2016
Neurological Surgery
2015
Hokkaido University
2015
Nippon Medical School
2015
The University of Tokyo
2001-2015
Jikei University School of Medicine
2015
Kyorin University
2015
In the present study, we examined signal transduction mechanism of reactive oxygen species (ROS) production and role ROS in angiotensin II-induced activation mitogen-activated protein kinases (MAPKs) rat neonatal cardiomyocytes. Among three MAPKs, c-Jun NH(2)-terminal kinase (JNK) p38 MAPK required for activation, as an NADPH oxidase inhibitor, diphenyleneiodonium, inhibited activation. The JNK was also by expression Galpha(12/13)-specific regulator G signaling (RGS) domain, a specific...
Sustained elevation of [Ca(2+)](i) has been implicated in many cellular events. We previously reported that alpha subunits G(12) family G proteins (Galpha(12/13)) participate sustained Ca(2+) influx required for the activation nuclear factor activated T cells (NFAT), a Ca(2+)-responsive transcriptional factor, rat neonatal cardiac fibroblasts. Here, we demonstrate Galpha(12/13)-mediated up-regulation canonical transient receptor potential 6 (TRPC6) channels participates and NFAT by...
In neonatal cardiomyocytes, activation of the G(q)-coupled alpha(1)-adrenergic receptor (alpha(1)AR) induces hypertrophy by activating mitogen-activated protein kinases, including c-Jun NH(2)-terminal kinase (JNK). Here, we show that JNK is essential for alpha(1)AR-induced hypertrophy, in hypertrophic responses, such as reorganization actin cytoskeleton and increased synthesis, could be blocked expressing JNK-binding domain JNK-interacting protein-1, a specific inhibitor JNK. We also...
Local hyperthermia has been widely used as physical therapy for a number of diseases such inflammatory osteoarticular disorders, tendinitis, and muscle injury. is clinically applied to improve blood lymphatic flow decrease swelling tissues (e.g., skeletal muscle). As repair following injury, the mechanisms underlying beneficial effects hyperthermia-induced are unknown. In this study, we investigated direct continuous heat stress on differentiation cultured mammalian myoblasts. Compared with...
Transfer of type-1 helper T-conditioned (Th1-conditioned) cells promotes functional recovery with enhanced axonal remodeling after spinal cord injury (SCI). This study explored the molecular mechanisms underlying beneficial effects pro-inflammatory Th1-conditioned SCI. The effect from interferon-γ (ifn-γ) knockout mice (ifn-γ−/− Th1 cells) on SCI was reduced. led to activation microglia (MG) and macrophages (MΦs), interleukin 10 (IL-10) upregulation. upregulation IL-10 reduced when ifn-γ−/−...
Epithelial-mesenchymal transition (EMT) is associated with tumor malignancy. The hedgehog-EMT pathway preferentially activated in diffuse-type gastric cancer (GC) compared intestinal-type GC; however, histological typing currently the only method for distinguishing these two major types of GC. We gene expression profiles 12 bone marrow-derived mesenchymal stem cell cultures and 5 GC tissue samples. Numerous upregulated or downregulated genes were identified GC, including CDH1, CDH2, VIM,...
<h3>Objectives:</h3> The aim of this study was to identify risk factors for rupture unruptured cerebral aneurysms (UCAs) in elderly Japanese patients aged 70 years or older. <h3>Methods:</h3> participants included all age older 3 prospective studies Japan (the Unruptured Cerebral Aneurysm Study [UCAS Japan], UCAS II, and the at Jikei University School Medicine). A total 1,896 with 2,227 UCAs were investigated. median mean follow-up periods 990 802.7 days, respectively. <h3>Results:</h3>...
In the present study, we examined roles of G(12), G(13), G(q), and G(i) in endothelin-1-induced hypertrophic responses. Endothelin-1 stimulation activated extracellular signal-regulated kinase (ERK) c-Jun NH(2)-terminal (JNK) cultured rat neonatal myocytes. The activation JNK, but not ERK, was inhibited by expression carboxyl terminal regions G alpha(12) alpha(13). JNK also alpha(12)/G alpha(13)-specific inhibitor regulator protein signaling (RGS) domain p115RhoGEF alpha(q)-specific RGS...
The oncogenic G12/13 subfamily of heterotrimeric G proteins transduces extracellular signals that regulate the actin cytoskeleton, cell cycle progression, and gene transcription. Previously, structural analyses fully functional Gα12/13 subunits have been hindered by insufficient amounts homogeneous, protein. Herein, we report substitution N-terminal helix Gαi1 for corresponding region Gα12 or Gα13 generated soluble chimeric (Gαi/12 Gαi/13) could be purified in sufficient crystallographic...
Journal Article Gene Expression Profiling of Human Mesenchymal Stem Cells for Identification Novel Markers in Early- and Late-Stage Cell Culture Get access Shihori Tanabe, Tanabe * Division Cellular Therapy Products, National Institute Health Sciences, Tokyo 158-8501, Japan *To whom correspondence should be addressed. Tel: +81-3-3700-1141, Fax: +81-3-3700-9217, E-mail: stanabe@nihs.go.jp Search other works by this author on: Oxford Academic PubMed Google Scholar Yoji Sato, Sato Takayoshi...
The role of T lymphocytes in central nervous system (CNS) injuries is controversial, with inconsistent results reported concerning the effects T-lymphocyte transfer on spinal cord injury (SCI). Here, we demonstrate that a specific subset enhances functional recovery after contusion SCI mice. Intraperitoneal adoptive type 1 helper (Th1)-conditioned cells 4 days promoted locomotor activity and tactile sensation concomitantly induced regrowth corticospinal tract serotonergic fibers. However,...
Epithelial-mesenchymal transition (EMT) plays an important role in the acquisition of cancer stem cell (CSC) feature and drug resistance, which are main hallmarks malignancy. Although previous findings have shown that several signaling pathways activated progression, precise mechanism EMT CSCs not fully understood. In this study, we focused on intestinal diffuse-type gastric (GC) analyzed gene expression public RNAseq data to understand molecular pathway regulation different subtypes cancer....
β1-Adrenergic receptor (β1AR) shows the resistance to agonist-induced internalization. However, β1AR can internalize as G protein-coupled kinase 2 (GRK2) is fused its carboxyl terminus. Internalization of and GRK2 fusion protein (β1AR/GRK2) dependent on dynamin but independent β-arrestin phosphorylation. The β1AR/GRK2 internalizes via clathrin-coated pits found co-localize with endosome that contains transferrin. proteins consisting various portions reveal residues 498–502 in...
G-protein (Gβγ)-mediated voltage-dependent inhibition of N- and P/Q-type Ca 2+ channels contributes to presynaptic short-term synaptic plasticity. The voltage dependence derives from the dissociation Gβγ inhibited channels, but underlying molecular biophysical mechanisms remain largely unclear. In this study we investigated role in process channel β subunit (Ca v β) a rigid α-helical structure between α-interacting domain (AID), primary docking site on α 1 subunit, pore-lining IS6 segment....
To investigate β-catenin (CTNNB1) signaling in cancer and stem cells, the gene expression pathway were analyzed using bioinformatics.The of catenin β 1 gene, which codes for β-catenin, was mesenchymal cells (MSCs) gastric (GC) cells. Beta-catenin mutation related proteins also cBioPortal Cancer Genomics HOMology modeling Complex Structure (HOMCOS) databases.The CTNNB1 up-regulated GC compared to MSCs. The EPH receptor A8 (EPHA8), synovial sarcoma translocation chromosome 18 (SS18),...
The coronavirus pathogenesis pathway, which consists of severe acute respiratory syndrome (SARS) infection and signaling pathways, including the interferon transforming growth factor beta mitogen-activated pathway kinase apoptosis inflammation is activated upon coronaviral infection. An artificial intelligence approach based on machine learning was utilized to develop models with images predict activation states. Data held in a database were analyzed Ingenuity Pathway Analysis (IPA), network...
Pertussis toxin (PTX) is recognized as a specific tool that uncouples receptors from G(i) and G(o) through ADP-ribosylation. During the study analyzing effects of PTX on Ang II type 1 receptor (AT1R) function in cardiac fibroblasts, we found increases number AT1Rs enhances AT1R-mediated response. Microarray analysis revealed induction interleukin (IL)-1beta among cytokines. Inhibition IL-1beta suppressed enhancement response by PTX. increased expression AT1R NF-kappaB, small GTP-binding...
Stem cell differentiation is regulated by multiple signaling events. Recent technical advances have revealed that differentiated cells can be reprogrammed into stem cells. The signals involved in programming are of major interest research. mechanisms regulating reprogramming and the subject intense study field life sciences. In this review, molecular interactions pathways related to discussed.