A5059295270- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Magnetic and transport properties of perovskites and related materials
- Multiferroics and related materials
- Bone Tissue Engineering Materials
- Advanced Condensed Matter Physics
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Electronic and Structural Properties of Oxides
- Ferroelectric and Piezoelectric Materials
- Collagen: Extraction and Characterization
- Polymer Surface Interaction Studies
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Physics of Superconductivity and Magnetism
- Corneal Surgery and Treatments
- Additive Manufacturing and 3D Printing Technologies
- biodegradable polymer synthesis and properties
- Magnetic Properties and Applications
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- X-ray Diffraction in Crystallography
- Tendon Structure and Treatment
- Nanoplatforms for cancer theranostics
- Rare-earth and actinide compounds
Toyo University
2024-2025
Institute of Science Tokyo
2025
The University of Tokyo
2025
Tokyo Medical and Dental University
2015-2024
Shibaura Institute of Technology
2024
Nihon University
2022
Nagasaki University
2022
Cornell University
2019
Case Western Reserve University
2019
Osaka University
1996-2017
The adsorption of proteins on poly(2-hydroxyethyl methacrylate) (PHEMA) brushes was systematically studied by quartz crystal microbalance (QCM) and fluorescence microscopy as a function graft density protein size. σ (chains/nm2) ranged from 0.007 (dilute or semidilute brush regime) to 0.7 (concentrated regime), the size 2 13 nm in an effective diameter. lowest-density (σ = 0.007) adsorbed all tested four proteins, while highest-density 0.7) none them. middle-density 0.06) showed intermediate...
To control the crosslinking rate of collagen gel, ethanol/water co-solvent was adopted for reaction solvent microfibril crosslinking. Collagen gel prepared by using EDC and NHS as coupling agents. Ethanol did not denaturate helical structure prevented hydrolysis EDC, but showed protonation carboxylate anions. In order to intra- interhelical crosslink triple helix, variations mole ratio carboxyl group/EDC/NHS, ethanol concentration were investigated. Increase in against group increased rate....
The critical behavior of the metal-insulator (MI) transition around ${x}_{c}\phantom{\rule{0ex}{0ex}}=\phantom{\rule{0ex}{0ex}}0.16$ has been investigated for single crystals ${\mathrm{La}}_{1\ensuremath{-}x}{\mathrm{Sr}}_{x}{\mathrm{MnO}}_{3}$ by measurements low-temperature resistivity and specific heat. electronic heat coefficient, $\ensuremath{\gamma}\phantom{\rule{0ex}{0ex}}=\phantom{\rule{0ex}{0ex}}3--5\mathrm{mJ}/{\mathrm{K}}^{2}\mathrm{mol}$, indicates minimal mass-renormalization...
Decellularized tissues, in which the extracellular matrix is isolated, have broad applications as implantable biomaterials and/or biological scaffolds for tissue repair, and show good clinical performance. characteristics, such their shape, structure, mechanical properties, activity, are strongly affected by decellularization protocol. The orthotopic implantation of decellularized a common procedure, typically induces cell infiltration (ECM) reconstruction resulting tissues that resemble...
An analytical processing design is proposed to accumulate nano-plastics diluted in water-based solvents and evaluate their individual IR spectral properties.
Many types of decellularized tissues have been studied and some commercially used in clinics. In this study, small-diameter vascular grafts were made using HHP to decellularize porcine radial arteries. One decellularization method, high hydrostatic pressure (HHP), has prepare the tissues. Low-temperature treatment was effective preserving collagen structures carotid The elastin mechanical properties HHP-decellularized arteries similar those untreated Xenogeneic transplantation (into rats)...
Abstract The decellularized cornea has received considerable attention for use as an artificial cornea. is free from cellular components and other immunogens, but maintains the integrity of extracellular matrix. However, ultrastructure yet to be demonstrated in detail. We investigated influence high hydrostatic pressure (HHP) on decellularization corneal its involvement transparency assessed vivo behaviour using two animal transplantation models, relation remodelling collagen fibrils....
Periodontal disease is chronic inflammation that leads to the destruction of tooth-supporting periodontal tissues. We devised a novel method ("cell transfer technology") cells onto scaffold surface and reported potential technique for regenerative medicine. The aim this study examine efficacy in regeneration fate transplanted cells. Human ligament stem (PDLSCs) were transferred decellularized amniotic membrane into defects rats. Regeneration tissues was examined by microcomputed tomography...
Abstract Following small‐diameter vascular grafting, blood vessels fail to retain excellent antithrombotic function and therefore require application of antithrombogenic drugs. We have previously reported early attachment endothelial cells the luminal surface high hydrostatic pressure (HHP)‐decellularized arteries after transplantation. In addition, graft retained by endothelialization remained open for several weeks. To fabricate tube grafts optimal size shape we evaluated decellularized...
The purpose of this study is to demonstrate the feasibility DALK using a decellularized corneal matrix obtained by HHP methodology. Porcine corneas were hydrostatically pressurized at 980 MPa 10°C for 10 minutes destroy cells, followed washing with EGM-2 medium remove cell debris. HHP-treated stained H-E assess efficacy decellularization. 300 μm thickness and 6.0 mm diameter was transplanted onto keratectomy wound. time course regeneration on evaluated haze grading score, fluorescein...
Tendons and ligaments are essential connective tissues that connect the muscle bone. Their recovery from injuries is known to be poor, highlighting crucial need for an effective therapy. A few reports have described development of artificial with sufficient strength human cells. In this study, we successfully generated a tendon-like tissue (bio-tendon) using induced pluripotent stem cells (iPSCs). We first differentiated iPSCs into mesenchymal (iPSC-MSCs) transfected them Mohawk (Mkx) obtain...
Abstract Soft gingiva is often compromised in gingival health; however, the underlying biological mechanisms remain unknown. Extracellular matrix (ECM) stiffness involved progression of various fibroblast-related inflammatory disorders via cellular mechanotransduction. Gingival might regulate mechanotransduction-mediated proinflammatory responses fibroblasts. This vitro study aims to investigate effects substrate on human fibroblasts (hGFs). The hGFs isolated from two healthy donors cultured...
Direct electronic structure measurements of a variety the colossal magnetoresistive oxides show presence pseudogap at Fermi energy E_F which drastically suppresses electron spectral function E_F. The is strong layer number samples (sample dimensionality) and strongly temperature dependent, with changes beginning ferromagnetic transition T_c. These trends are consistent major transport CMR oxides, implying direct relationship between transport, including "colossal" conductivity occur across...
Around the onset of labor, uterine sensitivity to oxytocin (OT) increases tremendously. Although this is considered reflect OT receptor (OTR) augmentation in myometrium, neither spatial expression OTR nor level message during course pregnancy have been investigated at molecular level. We examined localization and human myometrium by means situ hybridization, immunohistochemistry, Northern Western blotting. In term pregnant expressing smooth muscle cells are observed diffusely...
The meniscus functions as a load distributor and secondary stabilizer in the knee, loss of increases risk osteoarthritis. Freeze-thawed menisci are used clinical practice to replace defective menisci; however, disadvantages freeze-thawed tissues include disease transmission immune rejection. In this study, we decellularized using high hydrostatic pressure (HHP) compared with menisci. Porcine were either pressurized at 1,000 MPa for 10 min then washed DNase solution or frozen -80°C 2 days...
Abstract Hyaluronic acid (HA) has garnered much attention in the development of novel hydrogels. Hydrogels, as drug delivery systems, are very important tissue engineering applications. In this study, we developed a HA nanogel containing cholesterol and maleimide derivative (HAMICH) its corresponding crosslinked hydrogel (HAMICH gel) to encapsulate drugs for their subsequent release. HAMICH gels self-assemble into nanoparticles via hydrophobic interactions. Dynamic light scattering analysis...
OBJECTIVESThe aim of this research is to investigate the histological and mechanical properties decellularized aortic intima-media, a promising cardiovascular biomaterial.
One of the problems in dental implant treatment is lack periodontal ligament (PDL), which supports teeth, prevents infection, and transduces sensations such as chewiness. The objective present study was to develop a decellularized PDL for supporting an artificial tooth. To this end, we prepared mouse mandible bone with matrix by high hydrostatic pressure DNase detergent treatments evaluated its reconstruction vivo. After tooth extraction, implanted under subrenal capsule rat observed that...