A5032510897- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Cancer-related molecular mechanisms research
- dental development and anomalies
- Fibroblast Growth Factor Research
- Osteoarthritis Treatment and Mechanisms
- Proteoglycans and glycosaminoglycans research
- Bone health and treatments
- Tissue Engineering and Regenerative Medicine
- Oral and Maxillofacial Pathology
- Oral microbiology and periodontitis research
- Blood disorders and treatments
- Cancer therapeutics and mechanisms
- Lung Cancer Research Studies
- Endodontics and Root Canal Treatments
- Cancer Cells and Metastasis
- Bone Tissue Engineering Materials
- Dental Radiography and Imaging
- Protein Interaction Studies and Fluorescence Analysis
- Trigeminal Neuralgia and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Hepatitis B Virus Studies
- Protein Structure and Dynamics
- Dental Trauma and Treatments
- Multiple and Secondary Primary Cancers
The University of Texas Health Science Center at Houston
2021-2025
Tokyo Medical and Dental University
1995-2024
University of Michigan
2018-2021
The University of Tokyo
1999-2021
Osaka City University
2012
National Institutes of Health
2001
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2001
Kyushu University
2001
Kōchi University
1989
Bone marrow stromal cells (BMSCs) are versatile mesenchymal cell populations underpinning the major functions of skeleton, a majority which adjoin sinusoidal blood vessels and express C-X-C motif chemokine ligand 12 (CXCL12). However, how these activated during regeneration facilitate osteogenesis remains largely unknown. Cell-lineage analysis using Cxcl12-creER mice reveals that quiescent Cxcl12-creER+ perisinusoidal BMSCs differentiate into cortical bone osteoblasts solely regeneration. A...
Periodontal disease is one of the most common infectious diseases in adults and characterized by destruction tooth-supporting tissues. Mesenchymal stem cells (MSCs) comprise mesoderm-originating cell population, which has been studied used for therapy. However, because lower rate survival after MSC transplantation various models, paracrine functions MSCs have receiving increased attention as a regenerative mechanism. The aim this study was to investigate potential transplanted conditioned...
Formation of functional skeletal tissues requires highly organized steps mesenchymal progenitor cell differentiation. The dental follicle (DF) surrounding the developing tooth harbors cells for various differentiated constituting root-bone interface and coordinates eruption in a manner dependent on signaling by parathyroid hormone-related peptide (PTHrP) PTH/PTHrP receptor (PPR). However, identity DF how they are regulated PTHrP-PPR remain unknown. Here, we show that autocrine signal...
The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators formation. It is well-described that leptin receptor (LepR)+ perivascular provide a major source bone-forming osteoblasts adult and aged marrow. However, identity SSCs young how they coordinate active formation remains unclear. Here we show endosteal defined by fibroblast growth factor 3 (Fgfr3) osteoblast-chondrocyte transitional (OCT) identities with some...
Chondrocytes in the resting zone of postnatal growth plate are characterized by slow cell cycle progression, and encompass a population parathyroid hormone-related protein (PTHrP)-expressing skeletal stem cells that contribute to formation columnar chondrocytes. However, how these chondrocytes maintained remains undefined. We undertook genetic pulse-chase approach isolate cycling, label-retaining (LRCs) using chondrocyte-specific doxycycline-controllable Tet-Off system regulating expression...
Tooth eruption is a unique biological process by which highly mineralized tissues emerge into the outer world, and it occurs concomitantly with tooth root formation. These 2 processes have been considered independent phenomena; however, recent studies support theory that they are indeed intertwined. Dental mesenchymal progenitor cells in dental follicle lie at heart of coupling these processes, providing source for diverse formation functional periodontal attachment apparatus, while...
ABSTRACT The growth plate provides a substantial source of mesenchymal cells in the endosteal marrow space during endochondral ossification. current model postulates that group chondrocytes hypertrophic zone can escape from apoptosis and transform into eventually become osteoblasts an area beneath plate. is composed with various morphologies; particularly at periphery immediately adjacent to perichondrium are “borderline” chondrocytes, which align perpendicularly other chondrocytes. However,...
Periodontal disease is chronic inflammation that leads to the destruction of tooth-supporting periodontal tissues. We devised a novel method ("cell transfer technology") cells onto scaffold surface and reported potential technique for regenerative medicine. The aim this study examine efficacy in regeneration fate transplanted cells. Human ligament stem (PDLSCs) were transferred decellularized amniotic membrane into defects rats. Regeneration tissues was examined by microcomputed tomography...
In endochondral bone development, bone-forming osteoblasts and marrow stromal cells have dual origins in the fetal cartilage its surrounding perichondrium. However, how early perichondrial distinctively contribute to developing bones remain unidentified. Here we show using vivo cell-lineage analyses that Dlx5
Abstract During early mouse embryogenesis, cranial neural crest cells (CNCC) emigrate from the posterior midbrain and rhombomeres 1 2 of anterior hindbrain into first branchial arch‐derived maxillary mandibular processes there provide cell lineages for several phenotypes, including cartilage, bone, tooth. Here, we report that Sox9 Msx2 were coexpressed in a subpopulation CNCC during their migration. Because is transactivator chondrogenesis, Msx genes can act as transcriptional repressors,...
The parathyroid hormone 1 receptor (PTH1R) mediates the biologic actions of (PTH) and hormone-related protein (PTHrP). Here, we showed that salt-inducible kinases (SIKs) are key control skeletal downstream PTH1R this GPCR, when activated, inhibited cellular SIK activity. Sik gene deletion led to phenotypic changes were remarkably similar models increased signaling. In growth plate chondrocytes, PTHrP SIK3, ablation kinase in proliferating chondrocytes rescued perinatal lethality PTHrP-null...
The periodontium is essential for supporting the functionality of tooth, composed diversity mineralized and non-mineralized tissues such as cementum, periodontal ligament (PDL) alveolar bone. developmentally derived from dental follicle (DF), a fibrous tissue surrounding developing tooth bud. We previously showed through in vivo lineage-tracing experiments that DF contains mesenchymal progenitor cells expressing parathyroid hormone-related protein (PTHrP), which give rise to forming...
Abstract Alveolar bone supports and anchors teeth. The parathyroid hormone-related protein (PTHrP) pathway plays a key role in alveolar biology. Salt inducible kinases (SIKs) are important downstream regulators of PTH/PTHrP signaling the appendicular skeleton, where SIK inhibition increases formation trabecular mass. However, function these remains unknown. Here, we report critical for SIK2/SIK3 development, homeostasis, socket healing after tooth extraction. Inducible...
Objectives The periodontal ligament ( PDL ) has important roles in maintaining homeostasis, wound healing, and regeneration of tissues by supplying stem/progenitor cells. Periodontal stem cells PDLSC s) have mesenchymal cell MSC )‐like characteristics can be isolated from tissues. aim this study was to examine the effect three‐dimensional spheroid culture on s. Material Methods were cultured healthy teeth, spheroids formed pellet polypropylene tubes. proliferation s conventional...
Journal Article Aggregation and Chemical Reaction in Hen Lysozyme Caused by Heating at pH 6 Are Depressed Osmolytes, Sucrose Trehalose Get access Tadashi Ueda, Ueda 2 Graduate School of Pharmaceutical Sciences, Kyushu UniversityFukuoka 812-8582 2To whom correspondence should be addressed. Tel: +81-92-642-6663, Fax: +81-92-642-6667, E-mail: ueda@phar.kyushu-u.ac.jp Search for other works this author on: Oxford Academic PubMed Google Scholar Makiko Nagata, Nagata Taiji Imoto The Biochemistry,...
It has been unclear whether the increases in transcript accumulation for atrial natriuretic peptide (ANP) and brain (BNP) during pressure overload are caused by direct hemodynamic stress imposed on myocytes or mediated systemic hormonal factors. We examined levels regional distributions of ANP BNP mRNAs hypertrophied right ventricle produced experimental coarctation main pulmonary artery (PA) rats compared them with those skeletal alpha-actin mRNA, which is known to be a genetic marker...
Primary failure of eruption (PFE) is a genetic disorder exhibiting the cessation tooth eruption. Loss-of-function mutations in parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor (PTH/PTHrP receptor, PPR) were reported as underlying cause this humans. We showed PFE mouse model that PTHrP-PPR signaling responsible for normal dental follicle cell differentiation and However, mechanism defect remains undefined. In descriptive study, we aim to chronologically observe...
Bone development starts with condensations of undifferentiated mesenchymal cells that set a framework for future bones within the primordium. In endochondral pathway, inside condensation differentiate into chondrocytes and perichondrial in SOX9-dependent mechanism. However, identity outside how they participate developing remain undefined. Here we show surrounding contribute to both cartilage perichondrium, robustly generating chondrocytes, osteoblasts, marrow stromal bones. Single-cell...
Abstract For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied obtain a desirable arrangement of cells on scaffold materials. We developed novel method cell manipulation called “cell transfer technology”, enabling the cultured onto materials, and controlling topology. Here we show that using this technique, two different types can be transferred surface as stable double layers or patterned arrangements. Various combinations...
Our goal was to clarify the relationship between superior wall of mandibular canal and presence teeth. We also sought study structural changes after tooth loss. Twenty sides from 10 dry mandibles derived six males four females were used for this study. The age specimens at time death ranged 57 91 years. cut in midline resulting 20 hemi‐mandibles. teeth (from second premolar third molar) recorded each hemi‐mandible. body mandible divided into areas, that is, Areas 1–4. a cancellous bone...
Abstract Periodontitis is characterized by the chronic inflammation and destruction of tooth‐supporting tissues. Periodontal ligament stem cell (PDLSC) mesenchymal (MSC) population isolated from periodontal ligament, which key tissue for regeneration Although transplantation PDLSCs proposed as novel regenerative therapy, limited information available, regarding characteristic change during ex vivo expansion. In this study, we encountered morphological standard culture aimed to investigate in...