A5042230160- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Reproductive Biology and Fertility
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Gene expression and cancer classification
- Animal Genetics and Reproduction
- Prenatal Screening and Diagnostics
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Developmental Biology and Gene Regulation
- Metabolism and Genetic Disorders
- Sperm and Testicular Function
- Cancer-related gene regulation
- Tumors and Oncological Cases
- Extracellular vesicles in disease
- Chemokine receptors and signaling
- Marine Biology and Environmental Chemistry
- Precipitation Measurement and Analysis
Nara Medical University
2019-2025
Kyoto University
2011-2023
Japan Science and Technology Agency
2011-2016
Genetic Resources Center
2011
RIKEN Center for Computational Science
2008
Systems Biology Institute
2008
RIKEN Center for Integrative Medical Sciences
2005
The University of Tokyo
1999-2001
Tokyo University of Science
1999
Reconstitution of female germ cell development in vitro is a key challenge reproductive biology and medicine. We show here that (XX) embryonic stem cells induced pluripotent mice are into primordial cell-like (PGCLCs), which, when aggregated with gonadal somatic as reconstituted ovaries, undergo X-reactivation, imprint erasure, cyst formation, exhibit meiotic potential. Upon transplantation under mouse ovarian bursa, PGCLCs the ovaries mature germinal vesicle-stage oocytes, which then...
A systems-level understanding of a small but essential population cells in development or adulthood (e.g. somatic stem cells) requires accurate quantitative monitoring genome-wide gene expression, ideally from single cells. We report here strategy to globally amplify mRNAs for highly high-density oligonucleotide microarray analysis that combines number directional PCR cycles with subsequent linear amplification. Using this strategy, both the representation expression profiles and...
Specification of germ cell fate is fundamental in development. With a highly representative single-cell microarray and rigorous quantitative PCR analysis, we defined the genome-wide transcription dynamics that create primordial cells (PGCs) from epiblast, process exclusively segregates them their somatic neighbors. We also analyzed effect loss Blimp1, key transcriptional regulator, on these dynamics. Our analysis revealed PGC specification involves complex, yet ordered regulation large...
Highlights•WNT3 induces mesodermal factor T through β-CATENIN in epiblast-like cells•T is essential for primordial germ cell specification•T directly activates germline determinates Blimp1 and Prdm14 cells•BMP4 creates a permissive context to activate Prdm14SummaryGerm cells ensure reproduction heredity. In mice, (PGCs), the precursors spermatozoa oocytes, are induced pluripotent epiblast by BMP4 WNT3, yet underlying mechanism remains unclear. Here, using an vitro PGC specification system,...
Germ cell fate in mice is induced proximal epiblast cells at Embryonic Day (E) 6.5 by signaling molecules. Prdm1(also known as Blimp1)-positive lineage-restricted precursors of primordial germ (PGCs) initiate the formation a cluster that differentiates into Dppa3 (also stella)-positive PGCs from around E7.0 onwards extra-embryonic mesoderm. Around E7.5, these begin migrating towards definitive endoderm, with concomitant extensive epigenetic reprogramming. To gain more precise insight...
Cellular diversity of the brain is largely attributed to spatial and temporal heterogeneity progenitor cells. In mammalian cerebral development, it has been difficult determine how heterogeneous neural cells are, owing dynamic changes in their nuclear position gene expression. To address this issue, we systematically analyzed cDNA profiles a large number single at mid-embryonic stage mouse. By cluster analysis situ hybridization, have identified set genes that distinguishes between apical...
The Tudor domain–containing proteins (TDRDs) are an evolutionarily conserved family of involved in germ cell development. We show here that mice, TDRD5 is a novel component the intermitochondrial cements (IMCs) and chromatoid bodies (CBs), which cytoplasmic ribonucleoprotein granules RNA processing for spermatogenesis. Tdrd5-deficient males sterile because spermiogenic arrest at round spermatid stage, with occasional failure meiotic prophase. Without TDRD5, IMCs CBs disorganized,...
The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent (PSCs) is a key goal reproductive science. We show here that when aggregated with embryonic testicular somatic (reconstituted testes), primordial germ cell-like (PGCLCs) induced mouse differentiate into spermatogonia-like are expandable as resemble germline (GSCs), primary cell line SSC activity. Remarkably, GSC-like (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than...
Single-cell mRNA sequencing (RNA-seq) methods have undergone rapid development in recent years, and transcriptome analysis of relevant cell populations at single-cell resolution has become a key research area biomedical sciences. We here present 3-prime end (SC3-seq), practical methodology based on PCR amplification followed by 3-prime-end enrichment for highly quantitative, parallel cost-effective measurement gene expression single cells. The SC3-seq allows excellent quantitative mRNAs...
Commencing oogenesis In mice, embryonic stem cells and induced pluripotent have been shown to differentiate into primordial germ cell–like that can give rise functional oocytes. this system, Nagaoka et al. identified the gene Zglp1 as a necessary sufficient factor for conferring oogenic fate sexually undetermined cells. As downstream effector of bone morphogenetic protein signaling, conserved transcriptional regulator ZGLP1 activates program repressed by Polycomb activities, whereas retinoic...
The in vitro generation of germ cells from pluripotent stem (PSCs) can have a substantial effect on future reproductive medicine and animal breeding. A decade ago, gametogenesis was established the mouse. However, induction primordial cell-like (PGCLCs) to produce gametes has not been achieved any other species. Here, we demonstrate functional PGCLCs rat PSCs. We show that epiblast-like floating aggregates form PGCLCs. gonadal somatic support maturation epigenetic reprogramming When are...
The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse die soon after implantation. To explore the function of cells, RNA was conditionally knocked down vivo by shRNA. shRNA transgenic (NRi-Tg) mice were generated through formation germline chimeras with NRi-Tg embryonic stem cells. In E12.5 Cre-induced ER-Cre/NRi-Tg TNAP-Cre/NRi-Tg double-transgenic embryos, number alkaline phosphatase-positive SSEA1-positive PGCs...