A5056937280- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Axon Guidance and Neuronal Signaling
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Immune cells in cancer
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Color perception and design
- Bladder and Urothelial Cancer Treatments
- Ureteral procedures and complications
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Cardiovascular Effects of Exercise
- Medicinal Plant Pharmacodynamics Research
- Inflammasome and immune disorders
- Atmospheric and Environmental Gas Dynamics
- Molecular Biology Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Streptococcal Infections and Treatments
Okayama University
2022-2024
Kōchi University
2017-2024
Nagoya University
2014-2023
Morpho (United States)
2022
Kwansei Gakuin University
2021
RIKEN Center for Computational Science
2002-2004
RIKEN Center for Brain Science
2001-2004
Osaka University
2000-2001
Japan Science and Technology Agency
2000-2001
Keio University
2001
Mature neocortical layers all derive from the cortical plate (CP), a transient zone in dorsal telencephalon into which young neurons are continuously delivered. To understand cytogenetic and histogenetic events that trigger emergence of CP, we have used slice culture technique. Most divisions at ventricular surface generated paired cycling daughters (P/P divisions) majority P/P were asymmetric daughter cell behavior; they frequently sent one to non-surface (NS) position, subventricular...
Cellular diversity of the brain is largely attributed to spatial and temporal heterogeneity progenitor cells. In mammalian cerebral development, it has been difficult determine how heterogeneous neural cells are, owing dynamic changes in their nuclear position gene expression. To address this issue, we systematically analyzed cDNA profiles a large number single at mid-embryonic stage mouse. By cluster analysis situ hybridization, have identified set genes that distinguishes between apical...
Abstract During cerebral development, many types of neurons are sequentially generated by self-renewing progenitor cells called apical progenitors (APs). Temporal changes in AP identity thought to be responsible for neuronal diversity; however, the mechanisms underlying such remain largely unknown. Here we perform single-cell transcriptome analysis individual at different developmental stages, and identify a subset genes whose expression over time but is independent differentiation status....
The relationships between tissue-resident microglia and early macrophages, especially their lineage segregation outside the yolk sac, have been recently explored, providing a model in which conversion from macrophages seeds during brain development. However, spatiotemporal evidence to support such microglial seeding situ explain how it occurs has not obtained. By cell tracking via slice culture, intravital imaging, Flash tag-mediated or genetic labeling, we find that intraventricular CD206+...
Adult humans, like their nonhuman mammalian counterparts, harbor persistent neural progenitor cells in the forebrain ventricular lining. In absence of adequate surface markers, however, these have proven difficult to isolate for study. We previously identified and selected from both fetal adult rodent zone (VZ), by sorting transfected with plasmid DNA encoding gene green fluorescent protein driven early neuronal promoter Talpha1 tubulin (P/Talpha1:hGFP). now extended this approach purifying...
Cerebellar corticogenesis begins with the assembly of Purkinje cells into plate (PP) by embryonic day 14.5 (E14.5) in mice. Although dependence PP formation on secreted protein Reelin is well known and a prevailing model suggests that migrate along 'radial glial' fibers connecting ventricular pial surfaces, it not clear how behave response to initiate PP. Furthermore, what nascent look like vivo. When start axonogenesis must also be elucidated. We show generated E10.5 posterior...
Abstract Despite recent progress in the neural stem cell biology, their cellular characteristics have not been described well. We investigated various of cells (NSCs) vivo during CNS development, using FACS to identify NSCs. first examined stage‐dependent changes physical parameters, forward scatter (FSC) and side (SSC) profiles, NSCs from developing striatum, where they appear be active throughout life mammals. were divided into several fractions according FSC/SSC profile. With number...
In the developing cortex, postmigratory neurons accumulate in cortical plate (CP) to properly differentiate consolidating subtype identities. Microglia, despite their extensive surveying activity, temporarily disappear from midembryonic CP. However, mechanism and significance of this absence are unknown. Here, we show that microglia bidirectionally migrate via attraction by CXCL12 released meninges subventricular zone thereby exit Upon nonphysiological excessive exposure vivo or vitro, young...
In the developing central nervous system, cell departure from apical surface is initial and fundamental step to form 3D, organized architecture. Both delamination of differentiating cells repositioning progenitors generate outer radial glial (oRGs) contribute mammalian neocortical expansion; however, a comprehensive understanding their mechanisms lacking. Here, we demonstrate that Lzts1, molecule associated with microtubule components, promotes both events. neuronally committed cells, Lzts1...
Dendritic outgrowth in immature neurons is enhanced by neuronal activity and considered one of the mechanisms neural circuit optimization. It known that calcium signals affect transcriptional regulation cytoskeletal remodeling necessary for dendritic outgrowth. Here, we demonstrate activity-dependent signaling also controls mitochondrial homeostasis via AMP-activated protein kinase (AMPK) growing dendrites differentiating mouse hippocampal neurons. We found inhibition induced hypotrophy with...
Abstract Many tissues arise from pluripotent stem cells through cell‐type specification and maturation. In the bone marrow, primitive generate all different types of blood via sequential differentiation increasingly committed progenitor cells. Cell‐surface markers that clearly distinguish cells, restricted progenitors, differentiated progeny have enabled researchers to isolate these study regulatory mechanisms hematopoiesis. Neuronal appears involve similar mechanisms. However, neural are a...
Cell-producing events in developing tissues are mechanically dynamic throughout the cell cycle. In many epithelial systems, cells apicobasally tall, with nuclei and somata that adopt different apicobasal positions because move a cycle–dependent manner. This movement is apical during G2 phase basal G1 phase, whereas mitosis occurs at surface. These movements collectively referred to as interkinetic nuclear migration, such epithelia called "pseudostratified". The embryonic mammalian cerebral...
Abstract Spatiotemporally ordered production of cells is essential for brain development. Normally, most undifferentiated neural progenitor (NPCs) face the apical (ventricular) surface embryonic walls. Pathological detachment NPCs from and their invasion outer neuronal territories, i.e., formation NPC heterotopias, can disrupt overall structure brain. Although heterotopias have previously been observed in a variety experimental contexts, underlying mechanisms remain largely unknown....
For the understanding of histogenetic events in 3‐D retinal neuroepithelium, direct observation progenitor cells and their morphological changes is required. A slice culture method has been developed by which behavior single can be monitored. Although it believed that each cell loses its basal process while M phase, reported here retained throughout phase inherited one daughter cell, a neuron or cell. Daughter neurons used an for neuronal translocation positioning. In divisions produced two...
Abstract Progenitor cells that generate neuron pairs (“pair progenitor cells”) are implicated in mammalian cortical development, and their division has been thought to be “symmetric.” However, asymmetric growth of two sister neurons generated by the a pair cell would lead more efficient generation neuronal diversity cortex. To explore mechanisms which provide diversity, we examined molecular differences between clonal‐density culture. Time‐course analysis for acquisition markers...