A5085603616- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Developmental Biology and Gene Regulation
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
- Genetic and Kidney Cyst Diseases
- RNA Research and Splicing
- Olfactory and Sensory Function Studies
- Neonatal and fetal brain pathology
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Retinal Development and Disorders
- Sleep and Wakefulness Research
- Anesthesia and Neurotoxicity Research
- Cell death mechanisms and regulation
- Genetics and Neurodevelopmental Disorders
- RNA Interference and Gene Delivery
- Apelin-related biomedical research
- Congenital heart defects research
- Invertebrate Immune Response Mechanisms
Nagoya City University
2016-2025
National Institute for Physiological Sciences
2017-2025
Universitat de València
2010-2024
National Heart Lung and Blood Institute
2024
National Institutes of Health
2024
Instituto de Salud Carlos III
2024
Shiga University of Medical Science
2024
National Institutes of Natural Sciences
2020
Nagoya University
2012-2018
Mizuho (Japan)
2009-2012
In the adult brain, neuroblasts born in subventricular zone migrate from walls of lateral ventricles to olfactory bulb. How do these cells orient over such a long distance and through complex territories? Here we show that neuroblast migration parallels cerebrospinal fluid (CSF) flow. Beating ependymal cilia is required for normal CSF flow, concentration gradient formation guidance molecules, directional neuroblasts. Results suggest polarized epithelial contribute important vectorial...
Recent studies have revealed that the adult mammalian brain has capacity to regenerate some neurons after various insults. However, precise mechanism of insult-induced neurogenesis not been demonstrated. In normal brain, GFAP-expressing cells in subventricular zone (SVZ) lateral ventricles include a neurogenic cell population gives rise olfactory bulb only. Herein, we report evidence that, stroke, these are capable producing new outside bulbs. SVZ labeled by cell-type-specific viral...
Abstract Neural progenitor cells, including neural stem are a potential expandable source of graft material for transplantation aimed at repairing the damaged CNS. Here we present first evidence that in vitro‐expanded fetus‐derived neurosphere cells were able to generate neurons vivo and improve motor function upon into an adult rat spinal‐cord‐contusion injury model. As material, used cell‐enriched population was derived from embryonic spinal cord (E14.5) expanded vitro by formation. Nine...
In situ detection of neural progenitor cells including stem-like is essential for studying the basic mechanisms generation cellular diversity in CNS, upon which therapeutic treatments CNS injuries, degenerative diseases, and brain tumors may be based. We have generated rat monoclonal antibodies (Mab 14H1 14B8) that recognize an RNA-binding protein Musashi1, but not a Musashi1-related protein, Musashi2. The amino acid sequences at epitope sites these anti-Musashi1 Mabs are remarkably...
The subventricular zone (SVZ) of the adult brain contains neural stem cells that have capacity to regenerate new neurons after various insults. Brain ischemia causes damage tissue and induces regeneration together with angiogenesis. We previously reported that, ischemic injury in mice, SVZ-derived progenitor (NPCs) migrate into striatum, these NPCs are frequently associated blood vessels regenerating tissue. Here we studied role during more detail. BrdU administration experiments revealed...
Birth is one of the most important life events for animals. However, its significance in developmental process not fully understood. Here, we found that birth-induced alteration glutamine metabolism radial glia (RG), embryonic neural stem cells (NSCs), required acquisition quiescence and long-term maintenance postnatal NSCs. Preterm birth impairs this cellular process, leading to transient hyperactivation RG. Consequently, brain, NSC pool depleted neurogenesis decreased. We also quiescent RG...
Abstract The subventricular zone (SVZ) is the largest germinal in mature rodent brain, and it continuously produces young neurons that migrate to olfactory bulb. Neural stem cells this region generate migratory neuroblasts via highly proliferative transit-amplifying cells. Wnt/β-catenin signaling pathway partially regulates proliferation neuronal differentiation of neural progenitor embryonic brain. Here, we studied role β-catenin adult mouse SVZ. β-Catenin-dependent expression a...
To visualize and isolate live dopamine (DA)-producing neurons in the embryonic ventral mesencephalon, we generated transgenic mice expressing green fluorescent protein (GFP) under control of rat tyrosine hydroxylase gene promoter. In mice, GFP expression was observed developing DA containing hydroxylase. The outgrowth cue-dependent guidance GFP-labeled axons monitored vitro with brain culture systems. from tissue, cells fluorescence were sorted by fluorescence-activated cell sorting. More...
SUMMARY Neural stem cells in the subventricular zone (SVZ) of adult mammalian forebrain are a potential source neurons for neural tissue repair after brain insults such as ischemic stroke and traumatic injury (TBI). Recent studies show that neurogenesis ventricular (VZ) zebrafish telencephalon has features common with SVZ. Here, we established model to study injury-induced brain. We possesses remarkable capacity neuronal regeneration. Telencephalon prompted proliferation precursor (NPCs) VZ...
Neurogenesis in the subgranular zone of hippocampal dentate gyrus and olfactory bulbs continues into adulthood has been implicated cognitive function adult brain. The basal forebrain cholinergic system suggested to play a role regulating neurogenesis as well learning memory these regions. Herein, we report that highly polysialylated neural cell adhesion molecule (PSA‐NCAM)‐positive immature cells neuronal nuclei (NeuN)‐positive mature neurons bulb express multiple acetylcholine receptor...
The walls of the lateral ventricles contain largest germinal region in adult mammalian brain. subventricular zone (SVZ) these is an extensively studied model system for understanding behavior neural stem cells and regulation neurogenesis. Traditionally, studies have relied on classical sectioning techniques histological analysis. Here we present alternative approach, wholemount technique, which provides a comprehensive, en-face view this region. Compared to sections, wholemounts preserve...
Abstract Interleukin (IL)‐6 expression transiently increases in the acute phase of cerebral ischemia. To investigate physiological significance endogenous IL‐6 and to identify main signal pathway for action IL‐6, we administered anti‐mouse receptor monoclonal antibody (IL‐6RA), which blocks signaling, mice immediately after a 45‐min period middle artery occlusion (MCAO). At 6 h MCAO, IL‐6RA administration had resulted significant reduction amount phosphorylated transducer activator...
Perinatal hypoxia-ischemia (HI) has high rates of neurological deficits and mortality. So far, no effective treatment for HI brain injury been developed. In this study, we investigated the therapeutic effects stem cells from human exfoliated deciduous teeth (SHED) neonatal injury.Unilateral was induced in postnatal day 5 (P5) mice. Twenty-four hours later, SHED, skin fibroblasts, or serum-free conditioned medium derived these injected into injured brain. The cell transplantation injection on...
Interferon-alpha (IFN-α) is a proinflammatory cytokine that widely used for the treatment of chronic viral hepatitis and malignancy, because its immune-activating, antiviral, antiproliferative properties. However, long-term IFN-α frequently causes depression, which limits clinical utility. The precise molecular cellular mechanisms IFN-α-induced depression are not currently understood. Neural stem cells (NSCs) in hippocampus continuously generate new neurons, some evidence suggests decreased...
New neurons, continuously added in the adult olfactory bulb (OB) and hippocampus, are involved information processing neural circuits. Here, we show that synaptic pruning of adult-born neurons by microglia depends on phosphatidylserine (PS), whose exposure dendritic spines is inversely correlated with their input activity. To study role PS spine vivo, developed an inducible transgenic mouse line, which exposed masked a dominant-negative form milk fat globule-EGF-factor 8 (MFG-E8),...